 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 11, 2713-2724, Copyright © 1991 by Society for Neuroscience
The characterization and localization of the glutamate receptor subunit GluR1 in the rat brain
SW Rogers, TE Hughes, M Hollmann, GP Gasic, ES Deneris and S Heinemann
Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037.
The cloning of cDNAs that encode functional glutamate receptors makes it
possible to produce antibodies that can be used as high-affinity probes for
the localization and characterization of these receptors in the mammalian
brain. We have made antibodies to different regions of the first cloned
member of this family, GluR1, using bacterially overproduced antigen. On
Western blots, these antisera detect glycoprotein(s) of 105 kDa present in
crude membranes of the hippocampus and cerebellum. The 105-kDa band is
associated with postsynaptic densities, and it is observed in cultured
cells upon transfection with the GluR1 cDNA. Although glutamate receptors
are thought to be the most prevalent excitatory ligand-gated ion channel in
the mammalian brain, immunohistochemistry reveals that the receptors
recognized by these antisera are localized predominantly in neurons of the
cerebellum and some structures of the limbic system, including the
hippocampus, the central nucleus of the amygdala, and portions of the
septum. This pattern of expression is, in general, consistent with the
distribution of GluR1 mRNA as determined by in situ hybridization
histochemistry. Our results suggest that glutamate excitatory circuits
recognized by these antisera are predominantly found in regions of the
limbic system that are reciprocally interconnected.
This article has been cited by other articles:
|
|
|
|
 
J. Liu, I. Rasul, Y. Sun, G. Wu, L. Li, R. T. Premont, and W. Z. Suo
GRK5 Deficiency Leads to Reduced Hippocampal Acetylcholine Level via Impaired Presynaptic M2/M4 Autoreceptor Desensitization
J. Biol. Chem.,
July 17, 2009;
284(29):
19564 - 19571.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Olah, M. F. Jackson, H. Li, Y. Perez, H.-S. Sun, S. Kiyonaka, Y. Mori, M. Tymianski, and J. F. MacDonald
Ca2+-dependent induction of TRPM2 currents in hippocampal neurons
J. Physiol.,
March 1, 2009;
587(5):
965 - 979.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Oray, A. Majewska, and M. Sur
Effects of Synaptic Activity on Dendritic Spine Motility of Developing Cortical Layer V Pyramidal Neurons
Cereb Cortex,
May 1, 2006;
16(5):
730 - 741.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Li, S. Zhu, X. Song, Y. Shen, H. Chen, J. Yu, K. Yi, Y. Liu, V. J. Karplus, P. Wu, et al.
A Rice Glutamate Receptor-Like Gene Is Critical for the Division and Survival of Individual Cells in the Root Apical Meristem
PLANT CELL,
February 1, 2006;
18(2):
340 - 349.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. L. Palmer, L. Cotton, and J. M. Henley
The Molecular Pharmacology and Cell Biology of {alpha}-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid Receptors
Pharmacol. Rev.,
June 1, 2005;
57(2):
253 - 277.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. MIYASAKA, H. HOSOYA, S. TAKANO, M. OHTA, A. SEKIME, S. KANAI, T. MATSUI, and A. FUNAKOSHI
DIFFERENCES IN ETHANOL INGESTION BETWEEN CHOLECYSTOKININ-A RECEPTOR DEFICIENT AND -B RECEPTOR DEFICIENT MICE
Alcohol Alcohol.,
May 1, 2005;
40(3):
176 - 180.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. L. Meyer, N. Strutz, L. C. Gahring, and S. W. Rogers
Glutamate Receptor Subunit 3 Is Modified by Site-specific Limited Proteolysis Including Cleavage by {gamma}-Secretase
J. Biol. Chem.,
June 20, 2003;
278(26):
23786 - 23796.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-S. Li, Y. K. Cho, and D. V. Smith
Taste Responses of Neurons in the Hamster Solitary Nucleus Are Modulated by the Central Nucleus of the Amygdala
J Neurophysiol,
December 1, 2002;
88(6):
2979 - 2992.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Rajan, R. Preisig-Muller, E. Wischmeyer, R. Nehring, P. J Hanley, V. Renigunta, B. Musset, G. Schlichthorl, C. Derst, A. Karschin, et al.
Interaction with 14-3-3 proteins promotes functional expression of the potassium channels TASK-1 and TASK-3
J. Physiol.,
November 15, 2002;
545(1):
13 - 26.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. F. Sasaki, T. Rothe, L. S. Premkumar, S. Das, J. Cui, M. V. Talantova, H.-K. Wong, X. Gong, S. F. Chan, D. Zhang, et al.
Characterization and Comparison of the NR3A Subunit of the NMDA Receptor in Recombinant Systems and Primary Cortical Neurons
J Neurophysiol,
April 1, 2002;
87(4):
2052 - 2063.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. L. Meyer, L. C. Gahring, and S. W. Rogers
Nicotine Preconditioning Antagonizes Activity-dependent Caspase Proteolysis of a Glutamate Receptor
J. Biol. Chem.,
March 22, 2002;
277(13):
10869 - 10875.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. F. Chan and N. J. Sucher
An NMDA Receptor Signaling Complex with Protein Phosphatase 2A
J. Neurosci.,
October 15, 2001;
21(20):
7985 - 7992.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. C. Gahring, N. G. Carlson, E. L. Meyer, and S. W. Rogers
Cutting Edge: Granzyme B Proteolysis of a Neuronal Glutamate Receptor Generates an Autoantigen and Is Modulated by Glycosylation
J. Immunol.,
February 1, 2001;
166(3):
1433 - 1438.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Venugopal, R. Sharon, R. Abramovitz, A. Khasin, and R. Miskin
Plasminogen activator inhibitor-1 in cardiovascular cells: rapid induction after injecting mice with kainate or adrenergic agents
Cardiovasc Res,
February 1, 2001;
49(2):
476 - 483.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Opitz, S. Y. Grooms, M. V. L. Bennett, and R. S. Zukin
Remodeling of alpha -amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor subunit composition in hippocampal neurons after global ischemia
PNAS,
November 21, 2000;
97(24):
13360 - 13365.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. K. Friedman and A. R. Koudinov
Unilateral GluR2(B) Hippocampal Knockdown: A Novel Partial Seizure Model in the Developing Rat
J. Neurosci.,
November 1, 1999;
19(21):
9412 - 9425.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Wang, M. Post, and E. Cutz
Expression of Serotonin Receptor 2c in Rat Type II Pneumocytes
Am. J. Respir. Cell Mol. Biol.,
June 1, 1999;
20(6):
1175 - 1180.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
I. Everts, R. Petroski, P. Kizelsztein, V. I. Teichberg, S. F. Heinemann, and M. Hollmann
Lectin-Induced Inhibition of Desensitization of the Kainate Receptor GluR6 Depends on the Activation State and Can Be Mediated by a Single Native or Ectopic N-Linked Carbohydrate Side Chain
J. Neurosci.,
February 1, 1999;
19(3):
916 - 927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. C. Gahring and S. W. Rogers
Autoimmunity to Glutamate Receptors in Rasmussen's Encephalitis: A Rare Finding or The Tip of an Iceberg?
Neuroscientist,
September 1, 1998;
4(5):
373 - 379.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. W. Rogers, L. C. Gahring, A. C. Collins, and M. Marks
Age-Related Changes in Neuronal Nicotinic Acetylcholine Receptor Subunit alpha 4 Expression Are Modified by Long-Term Nicotine Administration
J. Neurosci.,
July 1, 1998;
18(13):
4825 - 4832.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Carder
Immunocytochemical Characterization of AMPA-Selective Glutamate Receptor Subunits: Laminar and Compartmental Distribution in Macaque Striate Cortex
J. Neurosci.,
May 1, 1997;
17(9):
3352 - 3363.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. G. Carlson, L. C. Gahring, R. E. Twyman, and S. W. Rogers
Identification of Amino Acids in the Glutamate Receptor, GluR3, Important for Antibody-binding and Receptor-specific Activation
J. Biol. Chem.,
April 25, 1997;
272(17):
11295 - 11301.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. H. Morrison, S. J. Siegel, A. H. Gazzaley, and G. W. Huntley
{blacksquare} REVIEW : Glutamate Receptors: Emerging Links Between Subunit Proteins and Specific Excitatory Circuits in Primate Hippocampus and Neocortex
Neuroscientist,
September 1, 1996;
2(5):
272 - 283.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. Velaz-Faircloth, A. Guadao-Ferraz, V. A. Henzi, and R. T. F. Jr.
Mammalian Brain-specific L-Proline Transporter
J. Biol. Chem.,
June 30, 1995;
270(26):
15755 - 15761.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. G. Wo and R. E. Oswald
A Topological Analysis of Goldfish Kainate Receptors Predicts Three Transmembrane Segments
J. Biol. Chem.,
February 3, 1995;
270(5):
2000 - 2009.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Rogers, P. Andrews, L. Gahring, T Whisenand, K Cauley, B Crain, T. Hughes, S. Heinemann, and J. McNamara
Autoantibodies to glutamate receptor GluR3 in Rasmussen's encephalitis
Science,
July 29, 1994;
265(5172):
648 - 651.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. Y. Grooms, T. Opitz, M. V. L. Bennett, and R. S. Zukin
Status epilepticus decreases glutamate receptor 2 mRNA and protein expression in hippocampal pyramidal cells before neuronal death
PNAS,
March 28, 2000;
97(7):
3631 - 3636.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. G. Garcia, K. Vasudevan, and A. Buonanno
The neuregulin receptor ErbB-4 interacts with PDZ-containing proteins at neuronal synapses
PNAS,
March 28, 2000;
97(7):
3596 - 3601.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
