 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 12, 4816-4833, Copyright © 1992 by Society for Neuroscience
Visualization of O-2A progenitor cells in developing and adult rat optic nerve by quisqualate-stimulated cobalt uptake
BP Fulton, JF Burne and MC Raff
Department of Anatomy and Developmental Biology, University College London, United Kingdom.
Some macroglial cells of the O-2A lineage express glutamate receptor
channels of the quisqualate/kainate type and take up extracellular cobalt
when activated by glutamate agonists. These cells can be identified both in
vitro and in situ following precipitation and intensification of the
intracellular cobalt. We have used this technique to characterize these
cells in the developing and adult rat optic nerve. In purified cultures of
optic nerve cells, O-2A progenitor cells and type 2 astrocytes took up
cobalt in the presence of quisqualate, while oligodendrocytes, type 1
astrocytes, and microglial cells did not. When whole optic nerves of
various postnatal ages were exposed to quisqualate and cobalt, a
subpopulation of glial cells took up cobalt. Cobalt uptake in vitro and in
situ was blocked by 6-cyano-7- nitroquinoxaline-2,3-dione. The number,
morphology, and spatial distribution of cobalt-filled cells in situ varied
with age. In perinatal nerves, 9% of glial cells took up cobalt. These
cells had a simple unipolar or bipolar morphology and were two to three
times more concentrated at the chiasm end than at the eye end of the nerve.
During subsequent development, this gradient disappeared and the
cobalt-filled cells became progressively more complex in morphology and
increased in number and density, reaching a peak toward the end of the
second postnatal week. The number subsequently declined to about 16,000
(7%) in the adult nerve. The processes of some cobalt-filled cells appeared
to contact nodes of Ranvier. All cobalt-filled cells in 2 1/2-week-old
optic nerves had a similar ultrastructural appearance and did not resemble
either mature oligodendrocytes or astrocytes. Our results suggest that the
cells stimulated by quisqualate to take up cobalt in the optic nerve are
the in vivo counterpart of O-2A progenitor cells. We found no evidence that
any of these cells are type 2 astrocytes.
This article has been cited by other articles:
|
|
|
|
 
F. Guo, J. Ma, E. McCauley, P. Bannerman, and D. Pleasure
Early Postnatal Proteolipid Promoter-Expressing Progenitors Produce Multilineage Cells In Vivo
J. Neurosci.,
June 3, 2009;
29(22):
7256 - 7270.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Kessaris, N. Pringle, and W. D Richardson
Specification of CNS glia from neural stem cells in the embryonic neuroepithelium
Phil Trans R Soc B,
January 12, 2008;
363(1489):
71 - 85.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Nishiyama
Polydendrocytes: NG2 Cells with Many Roles in Development and Repair of the CNS
Neuroscientist,
February 1, 2007;
13(1):
62 - 76.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. Raff
Intracellular Developmental Timers
Cold Spring Harb Symp Quant Biol,
January 1, 2007;
72(0):
431 - 435.
[Abstract]
[PDF]
|
|
|
|
|
|
|
B. Menn, J. M. Garcia-Verdugo, C. Yaschine, O. Gonzalez-Perez, D. Rowitch, and A. Alvarez-Buylla
Origin of oligodendrocytes in the subventricular zone of the adult brain.
J. Neurosci.,
July 26, 2006;
26(30):
7907 - 7918.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Wosik, F. Ruffini, G. Almazan, A. Olivier, J. Nalbantoglu, and J. P. Antel
Resistance of human adult oligodendrocytes to AMPA/kainate receptor-mediated glutamate injury
Brain,
December 1, 2004;
127(12):
2636 - 2648.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Peters and C. Sethares
Oligodendrocytes, their Progenitors and other Neuroglial Cells in the Aging Primate Cerebral Cortex
Cereb Cortex,
September 1, 2004;
14(9):
995 - 1007.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Stegmuller, H. Werner, K.-A. Nave, and J. Trotter
The Proteoglycan NG2 Is Complexed with alpha -Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptors by the PDZ Glutamate Receptor Interaction Protein (GRIP) in Glial Progenitor Cells. IMPLICATIONS FOR GLIAL-NEURONAL SIGNALING
J. Biol. Chem.,
January 31, 2003;
278(6):
3590 - 3598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. G. Tang, Y. M. Tokumoto, and M. C. Raff
Long-Term Culture of Purified Postnatal Oligodendrocyte Precursor Cells: Evidence for an Intrinsic Maturation Program That Plays Out over Months
J. Cell Biol.,
March 6, 2000;
148(5):
971 - 984.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Mi and B. A. Barres
Purification and Characterization of Astrocyte Precursor Cells in the Developing Rat Optic Nerve
J. Neurosci.,
February 1, 1999;
19(3):
1049 - 1061.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Ghiani, A. Eisen, X Yuan, R. DePinho, C. McBain, and V Gallo
Neurotransmitter receptor activation triggers p27(Kip1 )and p21(CIP1) accumulation and G1 cell cycle arrest in oligodendrocyte progenitors
Development,
January 2, 1999;
126(5):
1077 - 1090.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. Shi, A. Marinovich, and B. A. Barres
Purification and Characterization of Adult Oligodendrocyte Precursor Cells from the Rat Optic Nerve
J. Neurosci.,
June 15, 1998;
18(12):
4627 - 4636.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Wolswijk
Chronic Stage Multiple Sclerosis Lesions Contain a Relatively Quiescent Population of Oligodendrocyte Precursor Cells
J. Neurosci.,
January 15, 1998;
18(2):
601 - 609.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X Yuan, A. Eisen, C. McBain, and V Gallo
A role for glutamate and its receptors in the regulation of oligodendrocyte development in cerebellar tissue slices
Development,
January 8, 1998;
125(15):
2901 - 2914.
[Abstract]
[PDF]
|
|
|
|
|
|
|
A. VERKHRATSKY, R. K. ORKAND, and H. KETTENMANN
Glial Calcium: Homeostasis and Signaling Function
Physiol Rev,
January 1, 1998;
78(1):
99 - 141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-i. Sakakibara and H. Okano
Expression of Neural RNA-Binding Proteins in the Postnatal CNS: Implications of Their Roles in Neuronal and Glial Cell Development
J. Neurosci.,
November 1, 1997;
17(21):
8300 - 8312.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F.-B. Gao and M. Raff
Cell Size Control and a Cell-intrinsic Maturation Program in Proliferating Oligodendrocyte Precursor Cells
J. Cell Biol.,
September 22, 1997;
138(6):
1367 - 1377.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Matute, M. V. Sanchez-Gomez, L. Martinez-Millan, and R. Miledi
Glutamate receptor-mediated toxicity in optic nerve oligodendrocytes
PNAS,
August 5, 1997;
94(16):
8830 - 8835.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. Hernandez and J. D. O. Pena
The Optic Nerve Head in Glaucomatous Optic Neuropathy
Arch Ophthalmol,
March 1, 1997;
115(3):
389 - 395.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. Pende, T. L. Fisher, P. B. Simpson, J. T. Russell, J. Blenis, and V. Gallo
Neurotransmitter- and Growth Factor-Induced cAMP Response Element Binding Protein Phosphorylation in Glial Cell Progenitors: Role of Calcium Ions, Protein Kinase C, and Mitogen-Activated Protein Kinase/Ribosomal S6 Kinase Pathway
J. Neurosci.,
February 15, 1997;
17(4):
1291 - 1301.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L.-J. Chew, M. W. Fleck, P. Wright, S. E. Scherer, M. L. Mayer, and V. Gallo
Growth Factor-Induced Transcription of GluR1 Increases Functional AMPA Receptor Density in Glial Progenitor Cells
J. Neurosci.,
January 1, 1997;
17(1):
227 - 240.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A Webb, P Clark, J Skepper, A Compston, and A Wood
Guidance of oligodendrocytes and their progenitors by substratum topography
J. Cell Sci.,
January 8, 1995;
108(8):
2747 - 2760.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. W. Levison, C. Chuang, B. J. Abramson, and J. E. Goldman
The migrational patterns and developmental fates of glial precursors in the rat subventricular zone are temporally regulated
Development,
November 1, 1993;
119(3):
611 - 622.
[Abstract]
[PDF]
|
|
|
|
|
