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Journal of Neuroscience, Vol 12, 390-407, Copyright © 1992 by Society for Neuroscience
The dissonance mutation at the no-on-transient-A locus of D. melanogaster: genetic control of courtship song and visual behaviors by a protein with putative RNA-binding motifs
KG Rendahl, KR Jones, SJ Kulkarni, SH Bagully and JC Hall
Department of Biology, Brandeis University, Waltham, Massachusetts 02254.
Genetic and molecular results are here presented revealing that the
dissonance (diss) courtship song mutation is an allele of the no-on-
transient-A (nonA) locus of Drosophila melanogaster. diss (now called
nonAdiss) was originally isolated as a mutant with aberrant pulse song,
although it was then noted to exhibit defects in responses to visual
stimuli as well. The lack of transient spikes in the electroretinogram
(ERG) and optomotor blindness associated with nonAdiss are shown to be
similar to the visual abnormalities caused by the original nonA mutations.
nonAdiss failed to complement either the ERG or optomotor defects
associated with four other nonA mutations. However, all four of these nonA
mutants--which were isolated on visual criteria alone--sang a normal
courtship song. nonAdiss complemented at least three of the nonA mutations
with regard to the singing phenotype, as assessed by a new method for
temporal analysis of the male's pulse song. Both visual and song
abnormalities caused by nonAdiss were rescued by P-element- mediated
transformation with overlapping 11 and 16 kilobase (kb) fragments of
genomic DNA (originally cloned from the nonA locus by Jones and Rubin,
1990). Analysis of behavioral phenotypes in transformed flies carrying
mutagenized versions of the 11 kb genomic fragment (in a nonAdiss genomic
background) localized the rescuing DNA to a region containing an open
reading frame that encodes a polypeptide (NONA) with similarity to a family
of RNA-binding proteins. Immunohistochemical determination of NONA's
spatial and temporal expression revealed that it is localized to the nuclei
of cells in many neural and non-neural tissues, at all stages of the life
cycle after very early in development. Genetic connections between the
control of two quite different behaviors--reproductive and visual--are
discussed, along with precedences for generally expressed gene products
playing roles in specific behaviors.
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