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Journal of Neuroscience, Vol 12, 489-505, Copyright © 1992 by Society for Neuroscience
Ca2+ stores in Purkinje neurons: endoplasmic reticulum subcompartments demonstrated by the heterogeneous distribution of the InsP3 receptor, Ca(2+)-ATPase, and calsequestrin
K Takei, H Stukenbrok, A Metcalf, GA Mignery, TC Sudhof, P Volpe and P De Camilli
Department of Cell Biology, Yale University, New Haven, Connecticut 06510.
The nature of second messenger-responsive intracellular Ca2+ stores in
neurons remains open for discussion. Here, we demonstrate the existence in
Purkinje cells (PCs) of endoplastic reticulum (ER) subcompartments
characterized by an uneven distribution of three proteins involved in Ca2+
storage and release: the inositol 1,4,5-trisphosphate (InsP3) receptor,
Ca(2+)-ATPase, and calsequestrin. Ca(2+)-ATPase and the InsP3 receptor have
a widespread, although not identical, distribution throughout the ER.
Calsequestrin is localized throughout the smooth ER and is particularly
concentrated in pleiomorphic vesicles with a moderately electron-dense
core, which appear to represent a subcompartment of the smooth ER. In
double-labeling experiments many of these vesicles were unlabeled by InsP3
receptor antibodies. These results suggest a key role of the ER as an
intracellular Ca2+ store and demonstrate a possible structural basis for
distinct intracellular Ca2+ pools regulated by different second messengers.
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|
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|
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|
|
|
|
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|
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|
|
|
|
|
|
|
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78(4):
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[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
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22654 - 22661.
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[Full Text]
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|
|
|
|
|
|
|
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17(6):
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[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
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January 1, 1997;
17(1):
190 - 203.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
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271(52):
33493 - 33501.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
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October 1, 1996;
16(19):
5967 - 5978.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
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16(11):
3760 - 3774.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
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271(7):
3568 - 3574.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Galli, P. S. McPherson, and P. De Camilli
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January 26, 1996;
271(4):
2193 - 2198.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A Yamamoto, R Masaki, and Y Tashiro
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J. Cell Sci.,
January 7, 1996;
109(7):
1727 - 1738.
[Abstract]
[PDF]
|
|
|
|
|
|
|
B G Frenguelli and R Malinow
Fluctuations in intracellular calcium responses to action potentials in single en passage presynaptic boutons of layer V neurons in neocortical slices.
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January 1, 1996;
3(2-3):
150 - 159.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. R. Schiller, R. E. Mains, and B. A. Eipper
A Neuroendocrine-specific Protein Localized to the Endoplasmic Reticulum by Distal Degradation
J. Biol. Chem.,
November 3, 1995;
270(44):
26129 - 26138.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. K. Joseph, C. Lin, S. Pierson, A. P. Thomas, and A. R. Maranto
Heteroligomers of Type-I and Type-III Inositol Trisphosphate Receptors in WB Rat Liver Epithelial Cells
J. Biol. Chem.,
October 6, 1995;
270(40):
23310 - 23316.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R Rizzuto, M Brini, M Murgia, and T Pozzan
Microdomains with high Ca2+ close to IP3-sensitive channels that are sensed by neighboring mitochondria
Science,
October 29, 1993;
262(5134):
744 - 747.
[Abstract]
[PDF]
|
|
|
|
|
|
|
Y. Nemoto, B. G. Kearns, M. R. Wenk, H. Chen, K. Mori, J. G. Alb Jr., P. De Camilli, and V. A. Bankaitis
Functional Characterization of a Mammalian Sac1 and Mutants Exhibiting Substrate-specific Defects in Phosphoinositide Phosphatase Activity
J. Biol. Chem.,
October 27, 2000;
275(44):
34293 - 34305.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Nemoto, M. R. Wenk, M. Watanabe, L. Daniell, T. Murakami, N. Ringstad, H. Yamada, K. Takei, and P. De Camilli
Identification and Characterization of a Synaptojanin 2 Splice Isoform Predominantly Expressed in Nerve Terminals
J. Biol. Chem.,
October 26, 2001;
276(44):
41133 - 41142.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
