 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 12, 800-809, Copyright © 1992 by Society for Neuroscience
Cholinergic inhibition of short (outer) hair cells of the chick's cochlea
PA Fuchs and BW Murrow
Department of Physiology, University of Colorado School of Medicine, Denver 80262.
Cochlear hair cells are thought to be inhibited by the release of ACh from
efferent neurons. Several studies have implicated Ca2+ as a postsynaptic
intermediary in hair cell inhibition, but its role remains unproven. We
have made whole-cell, tight-seal recordings from single short hair cells
(the avian analog of outer hair cells in the mammalian cochlea), isolated
from the chick's cochlea, to determine the mechanism of cholinergic
inhibition. These cells hyperpolarized upon exposure to ACh, although a
brief depolarization preceded the much larger, longer- lasting
hyperpolarization. In voltage clamp ACh evoked an outward current that
reversed in sign near the K+ equilibrium potential. A small, transient
inward current preceded the predominant outward current. The ACh-evoked K+
current depended on Ca2+ in the external saline, or could be prevented when
the cell was dialyzed with the rapid Ca2+ buffer BAPTA. In BAPTA-loaded
cells a residual inward current was seen. This activated with very little
delay upon exposure of the cell to ACh and reversed near 0 mV membrane
potential. Thus, the hair cell ACh receptor appears to be a nonspecific
cation channel through which Ca2+ enters and triggers the opening of nearby
Ca(2+)-activated K+ channels. However, the ACh-evoked K+ channels are not
the same as the "maxi" K+ channels activated by Ca2+ influx through
voltage-gated Ca2+ channels in these same cells.
This article has been cited by other articles:
|
|
|
|
 
R. Boyle, R. D. Rabbitt, and S. M. Highstein
Efferent Control of Hair Cell and Afferent Responses in the Semicircular Canals
J Neurophysiol,
September 1, 2009;
102(3):
1513 - 1525.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. G. Sadeghi, J. M. Goldberg, L. B. Minor, and K. E. Cullen
Efferent-Mediated Responses in Vestibular Nerve Afferents of the Alert Macaque
J Neurophysiol,
February 1, 2009;
101(2):
988 - 1001.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-H. Kong, J. P. Adelman, and P. A. Fuchs
Expression of the SK2 calcium-activated potassium channel is required for cholinergic function in mouse cochlear hair cells
J. Physiol.,
November 15, 2008;
586(22):
5471 - 5485.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. E. Vetter, E. Katz, S. F. Maison, J. Taranda, S. Turcan, J. Ballestero, M. C. Liberman, A. B. Elgoyhen, and J. Boulter
The {alpha}10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system
PNAS,
December 18, 2007;
104(51):
20594 - 20599.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Lee, O. Briklin, H. Hiel, and P. Fuchs
Calcium-dependent inactivation of calcium channels in cochlear hair cells of the chicken
J. Physiol.,
September 15, 2007;
583(3):
909 - 922.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. F. Maison, D. E. Vetter, and M. C. Liberman
A Novel Effect of Cochlear Efferents: In Vivo Response Enhancement Does Not Require {alpha}9 Cholinergic Receptors
J Neurophysiol,
May 1, 2007;
97(5):
3269 - 3278.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. F. Maison, L. L. Parker, L. Young, J. P. Adelman, J. Zuo, and M. C. Liberman
Overexpression of SK2 Channels Enhances Efferent Suppression of Cochlear Responses Without Enhancing Noise Resistance
J Neurophysiol,
April 1, 2007;
97(4):
2930 - 2936.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Holt, A. Lysakowski, and J. M. Goldberg
Mechanisms of Efferent-Mediated Responses in the Turtle Posterior Crista
J. Neurosci.,
December 20, 2006;
26(51):
13180 - 13193.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Akil, J. Chang, H. Hiel, J.-H. Kong, E. Yi, E. Glowatzki, and L. R. Lustig
Progressive Deafness and Altered Cochlear Innervation in Knock-Out Mice Lacking Prosaposin
J. Neurosci.,
December 13, 2006;
26(50):
13076 - 13088.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. V. Derbenev, C. L. Linn, and P. S. Guth
Muscarinic ACh Receptor Activation Causes Transmitter Release From Isolated Frog Vestibular Hair Cells
J Neurophysiol,
November 1, 2005;
94(5):
3134 - 3142.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Goutman, P. A. Fuchs, and E. Glowatzki
Facilitating efferent inhibition of inner hair cells in the cochlea of the neonatal rat
J. Physiol.,
July 1, 2005;
566(1):
49 - 59.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Gomez-Casati, P. A Fuchs, A. B. Elgoyhen, and E. Katz
Biophysical and pharmacological characterization of nicotinic cholinergic receptors in rat cochlear inner hair cells
J. Physiol.,
July 1, 2005;
566(1):
103 - 118.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Dawkins, S. L. Keller, and W. F. Sewell
Pharmacology of Acetylcholine-Mediated Cell Signaling in the Lateral Line Organ Following Efferent Stimulation
J Neurophysiol,
May 1, 2005;
93(5):
2541 - 2551.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Duzhyy, M. Harvey, and B. Sokolowski
A Secretory-type Protein, Containing a Pentraxin Domain, Interacts with an A-type K+ Channel
J. Biol. Chem.,
April 15, 2005;
280(15):
15165 - 15172.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. S. G. Geleoc, J. R. Risner, and J. R. Holt
Developmental Acquisition of Voltage-Dependent Conductances and Sensory Signaling in Hair Cells of the Embryonic Mouse Inner Ear
J. Neurosci.,
December 8, 2004;
24(49):
11148 - 11159.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Lioudyno, H. Hiel, J.-H. Kong, E. Katz, E. Waldman, S. Parameshwaran-Iyer, E. Glowatzki, and P. A. Fuchs
A "Synaptoplasmic Cistern" Mediates Rapid Inhibition of Cochlear Hair Cells
J. Neurosci.,
December 8, 2004;
24(49):
11160 - 11164.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Marcotti, S. L Johnson, and C. J Kros
A transiently expressed SK current sustains and modulates action potential activity in immature mouse inner hair cells
J. Physiol.,
November 1, 2004;
560(3):
691 - 708.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Katz, A. B. Elgoyhen, M. E. Gomez-Casati, M. Knipper, D. E. Vetter, P. A. Fuchs, and E. Glowatzki
Developmental Regulation of Nicotinic Synapses on Cochlear Inner Hair Cells
J. Neurosci.,
September 8, 2004;
24(36):
7814 - 7820.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. H. A. Sokolowski, Y. Sakai, M. C. Harvey, and D. E. Duzhyy
Identification and Localization of an Arachidonic Acid-Sensitive Potassium Channel in the Cochlea
J. Neurosci.,
July 14, 2004;
24(28):
6265 - 6276.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Nie, H. Song, M.-F. Chen, N. Chiamvimonvat, K. W. Beisel, E. N. Yamoah, and A. E. Vazquez
Cloning and Expression of a Small-Conductance Ca2+-Activated K+ Channel From the Mouse Cochlea: Coexpression with {alpha}9/{alpha}10 Acetylcholine Receptors
J Neurophysiol,
April 1, 2004;
91(4):
1536 - 1544.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Holt, M. Lioudyno, and P. S. Guth
A Pharmacologically Distinct Nicotinic ACh Receptor Is Found in a Subset of Frog Semicircular Canal Hair Cells
J Neurophysiol,
September 1, 2003;
90(3):
1526 - 1536.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Oliver, M. Knipper, C. Derst, and B. Fakler
Resting Potential and Submembrane Calcium Concentration of Inner Hair Cells in the Isolated Mouse Cochlea Are Set by KCNQ-Type Potassium Channels
J. Neurosci.,
March 15, 2003;
23(6):
2141 - 2149.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. F. Maison, A. E. Luebke, M. C. Liberman, and J. Zuo
Efferent Protection from Acoustic Injury Is Mediated via alpha 9 Nicotinic Acetylcholine Receptors on Outer Hair Cells
J. Neurosci.,
December 15, 2002;
22(24):
10838 - 10846.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. Luebke and P. K. Foster
Variation in Inter-Animal Susceptibility to Noise Damage Is Associated with alpha 9 Acetylcholine Receptor Subunit Expression Level
J. Neurosci.,
May 15, 2002;
22(10):
4241 - 4247.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Oliver, J. Ludwig, E. Reisinger, W. Zoellner, J. P. Ruppersberg, and B. Fakler
Memantine Inhibits Efferent Cholinergic Transmission in the Cochlea by Blocking Nicotinic Acetylcholine Receptors of Outer Hair Cells
Mol. Pharmacol.,
July 1, 2001;
60(1):
183 - 189.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. B. Elgoyhen, D. E. Vetter, E. Katz, C. V. Rothlin, S. F. Heinemann, and J. Boulter
alpha 10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells
PNAS,
March 1, 2001;
(2001)
51622798.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. Yoshida, M. C. Liberman, M. C. Brown, and W. F. Sewell
Fast, But Not Slow, Effects of Olivocochlear Activation Are Resistant to Apamin
J Neurophysiol,
January 1, 2001;
85(1):
84 - 88.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Blanchet, C. Erostegui, M. Sugasawa, and D. Dulon
Gentamicin blocks ACh-evoked K+ current in guinea-pig outer hair cells by impairing Ca2+ entry at the cholinergic receptor
J. Physiol.,
June 15, 2000;
525(3):
641 - 654.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Brichta and J. M. Goldberg
Responses to Efferent Activation and Excitatory Response-Intensity Relations of Turtle Posterior-Crista Afferents
J Neurophysiol,
March 1, 2000;
83(3):
1224 - 1242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Yoshida, M. C. Liberman, M. C. Brown, and W. F. Sewell
Gentamicin Blocks Both Fast and Slow Effects of Olivocochlear Activation in Anesthetized Guinea Pigs
J Neurophysiol,
December 1, 1999;
82(6):
3168 - 3174.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Z. Z. He and P. Dallos
Development of Acetylcholine-Induced Responses in Neonatal Gerbil Outer Hair Cells
J Neurophysiol,
March 1, 1999;
81(3):
1162 - 1170.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. L. Tredway, J.-Z. Guo, and V. A. Chiappinelli
N-Type Voltage-Dependent Calcium Channels Mediate the Nicotinic Enhancement of GABA Release in Chick Brain
J Neurophysiol,
February 1, 1999;
81(2):
447 - 454.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kehoe and J. M. McIntosh
Two Distinct Nicotinic Receptors, One Pharmacologically Similar to the Vertebrate alpha 7-Containing Receptor, Mediate Cl Currents in Aplysia Neurons
J. Neurosci.,
October 15, 1998;
18(20):
8198 - 8213.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. E. Armstrong and W. M. Roberts
Electrical Properties of Frog Saccular Hair Cells: Distortion by Enzymatic Dissociation
J. Neurosci.,
April 15, 1998;
18(8):
2962 - 2973.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. L. da Costa, A. Chibois, J.-P. Erre, C. Blanchet, R. C. de Sauvage, and J.-M. Aran
Fast, Slow, and Steady-State Effects of Contralateral Acoustic Activation of the Medial Olivocochlear Efferent System in Awake Guinea Pigs: Action of Gentamicin
J Neurophysiol,
October 1, 1997;
78(4):
1826 - 1836.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Dallos, D. Z. Z. He, X. Lin, I. Sziklai, S. Mehta, and B. N. Evans
Acetylcholine, Outer Hair Cell Electromotility, and the Cochlear Amplifier
J. Neurosci.,
March 15, 1997;
17(6):
2212 - 2226.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Rogers, L. M. Colquhoun, J. W. Patrick, and J. A. Dani
Calcium Flux Through Predominantly Independent Purinergic ATP and Nicotinic Acetylcholine Receptors
J Neurophysiol,
March 1, 1997;
77(3):
1407 - 1417.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Lena and J.-P. Changeux
Role of Ca2+ Ions in Nicotinic Facilitation of GABA Release in Mouse Thalamus
J. Neurosci.,
January 15, 1997;
17(2):
576 - 585.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. S. Sridhar, M. C. Brown, and W. F. Sewell
Unique Postsynaptic Signaling at the Hair Cell Efferent Synapse Permits Calcium to Evoke Changes on Two Time Scales
J. Neurosci.,
January 1, 1997;
17(1):
428 - 437.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. B. Elgoyhen, D. E. Vetter, E. Katz, C. V. Rothlin, S. F. Heinemann, and J. Boulter
alpha 10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells
PNAS,
March 13, 2001;
98(6):
3501 - 3506.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
