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Journal of Neuroscience, Vol 13, 1010-1021, Copyright © 1993 by Society for Neuroscience
Pairing the cholinergic agonist carbachol with patterned Schaffer collateral stimulation initiates protein synthesis in hippocampal CA1 pyramidal cell dendrites via a muscarinic, NMDA-dependent mechanism
S Feig and P Lipton
Department of Anatomy, University of Wisconsin Medical School, Madison 53706.
Effects of afferent stimulation on local synthesis of protein in CA1
pyramidal cell dendrites were studied using light microscope
autoradiography. Tissue was fixed with paraformaldehyde immediately after 3
min exposure to 3H-leucine in order to trap 3H associated with
macromolecules. The rate of 3H-leucine incorporation into dendrites of
resting hippocampal slices was 10% the rate of incorporation into cell
somata. Ninety percent of the incorporation into the somata was inhibited
by cycloheximide (300 microM); none of the incorporation into dendrites was
blocked by cycloheximide. Thus, there is no measurable extramitochondrial
synthesis of protein in the dendrites of the resting slice. Slices were
exposed to 50 microM carbachol and the Schaffer collateral afferents to the
CA1 pyramidal cells were stimulated intermittently at 10 Hz over a 20 min
period. In this case, 3H incorporation into dendrites was increased almost
threefold over resting levels, with no effect on label over the cell
somata. There was no associated increase in uptake of free 3H-leucine, and
the increase in label was completely blocked by cycloheximide. Thus,
associating carbachol and afferent stimulation appears to activate de novo
protein synthesis in the dendrites. Neither the carbachol alone nor the
Schaffer collateral stimulation alone increased synthesis. The activation
of dendrite synthesis was completely blocked by 5 microM atropine, and also
by 50 microM D-aminophosphonovalerate. It did not occur when carbachol was
paired with steady stimulation of the Schaffer collaterals at 1 Hz for 20
min, rather than with the patterned high- frequency stimulation. Thus,
associating a cholinergic agonist with a level of neural activity that
occurs in CA3 and CA1 pyramidal cells during exploratory behavior (Muller
et al., 1987) initiates local protein synthesis in target dendrites. This
effect is dependent on muscarinic cholinergic receptors and NMDA-type
glutamate receptors. The possible relationship of this phenomenon to
mechanisms of learning is discussed.
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