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Journal of Neuroscience, Vol 13, 2982-2992, Copyright © 1993 by Society for Neuroscience
Quantitative localization of AMPA/kainate and kainate glutamate receptor subunit immunoreactivity in neurochemically identified subpopulations of neurons in the prefrontal cortex of the macaque monkey
JC Vickers, GW Huntley, AM Edwards, T Moran, SW Rogers, SF Heinemann and JH Morrison
Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, New York 10029.
Excitatory amino acid transmission has been proposed as the principal
synaptic mechanism for distribution of information through corticocortical
and thalamocortical pathways. The following study utilized a double
labeling paradigm, using antibodies that recognize non-NMDA ionotropic
glutamate receptor subunits and other neuronal markers, to further define,
quantitatively, the subclasses of neurons that contain immunoreactivity for
the AMPA/kainate and kainate receptor subunits in the monkey prefrontal
cortex. Double labeling with an antibody that recognizes common epitopes in
AMPA/kainate subunits GluR2 and GluR3 (GluR2/3) in combination with an
antibody that recognizes the kainate receptor subunits GluR5, GluR6, and
GluR7 (GluR5/6/7) demonstrated that immunoreactivity for these two receptor
classes was highly colocalized in a great majority of the pyramidal neurons
in this region but present in only a minority of neurochemically identified
subclasses of GABAergic interneurons. Furthermore, GluR2/3 immunoreactivity
had principally a somatic distribution whereas GluR5/6/7 labeling was
predominately found in the perikarya and/or particular dendritic domains.
In contrast, intense GluR1 labeling was observed in a small subpopulation
of interneurons and low GluR1 immunoreactivity was present in many other
cortical neurons. These results demonstrate that there is a high degree of
specificity in the distribution of AMPA/kainate and kainate receptor-class
proteins to subclasses of neurons within the neocortex. A neuron's
combination of excitatory amino acid receptor subunits may regulate its
response to excitatory inputs and further defines the role of identified
subclasses of neurons in the complex circuitry of the cerebral cortex and
may also indicate the basis for the apparent cellular selectivity of
excitotoxic degenerative processes.
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