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Journal of Neuroscience, Vol 14, 1202-1212, Copyright © 1994 by Society for Neuroscience
A novel GABA receptor on bipolar cell terminals in the tiger salamander retina
PD Lukasiewicz, BR Maple and FS Werblin
Department of Ophthalmology, Washington University, St. Louis, Missouri 63110.
We studied the pharmacology of the GABA receptors on bipolar cell terminals
in the retinal slice preparation. Whole-cell patch-clamp recordings were
made from the somas of bipolar cells and GABA was puffed near their
terminals, after synaptic transmission was blocked. GABA puffs evoked a
large chloride current that was reduced by picrotoxin, but in many cells
this current was insensitive to blockade by the competitive GABAA receptor
antagonists bicuculline and SR95531. Pentobarbital, an enhancer of GABAA
receptor-mediated responses, did not significantly increase the magnitude
of the current responses to GABA puffed at the bipolar cell terminals. To
confirm the effectiveness of GABAA antagonists and pentobarbital in the
slice preparation, we measured GABA currents in ganglion cells. In contrast
to bipolar cells, the ganglion cell GABA responses were strongly reduced by
both bicuculline and SR95531. In addition, pentobarbital strongly enhanced
the action of GABA at the ganglion cells. The isomeric GABA agonists cis-
and transaminocrotonic acid (CACA and TACA), elicited picrotoxin- sensitive
currents in both bipolar and ganglion cells. TACA was more effective than
CACA at both cell types. In bipolar cells, TACA and CACA currents were
relatively resistant to bicuculline blockade, but in ganglion cells both
currents were reduced by bicuculline. GABA receptors on bipolar terminals
appear to be pharmacologically different from the GABA receptors found on
ganglion cell dendrites. The bipolar cell terminal GABA receptor
pharmacology is similar to the pharmacology reported for the rho 1 GABA
receptor subunit that was isolated from retina and expressed in Xenopus
oocytes (Cutting et al., 1991; Polenzani et al., 1991; Shimada et al.,
1992). This receptor, which is both bicuculline and pentobarbital
insensitive, has been called the GABAC receptor (Johnston, 1986; Shimada et
al., 1992). However, some bipolar cells were somewhat sensitive to blockade
by bicuculline, suggesting that these cells had both GABAA and GABAC
receptors on their bipolar terminals.
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