 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 14, 2357-2364, Copyright © 1994 by Society for Neuroscience
The developmental increase in ACh current densities on rat sympathetic neurons correlates with changes in nicotinic ACh receptor alpha-subunit gene expression and occurs independent of innervation
A Mandelzys, B Pie, ES Deneris and E Cooper
Department of Physiology, McGill University, Montreal, Quebec, Canada.
Determining factors that control the expression of neurotransmitter
receptors and the mechanisms by which these factors operate is essential to
understand how synapses form during development and how receptor numbers
change in the adult. In this study, we have investigated one such factor,
the influence of innervation, on the developmental expression of nicotinic
ACh receptors (nAChRs) on neonatal rat sympathetic neurons, both in terms
of ACh current densities, and in terms of mRNA levels for the transcripts
that encode these receptors. To date, nine genes have been cloned that
encode neuronal nAChRs subunits in mammals. We demonstrate that mRNA
encoding five nAChR subunits, alpha 3, alpha 5, alpha 7, beta 2, and beta
4, are present in neonatal rat sympathetic neurons. We show that mRNA
levels for alpha 3 and alpha 7 subunits increase by more than threefold
over the first 2 postnatal weeks, a period when most synapses are forming
on the neurons; however, we observed no significant change in mRNA levels
for alpha 5, beta 2, or beta 4. Using whole-cell voltage-clamp techniques,
we show that the increase in alpha-subunit mRNA correlates with increases
in ACh current densities, which double over the same period. To investigate
the role of innervation, we cut the preganglionic nerve at birth and
measured subunit mRNA levels and ACh current densities in denervated
neurons 1-2 weeks later. Our results indicate that the preganglionic nerve
differentially affects the mRNA level for the five nAChR transcripts, yet
it has little influence on the developmental increase in ACh current
densities.
This article has been cited by other articles:
|
|
|
|
 
B. Lendvai and E. S. Vizi
Nonsynaptic Chemical Transmission Through Nicotinic Acetylcholine Receptors
Physiol Rev,
April 1, 2008;
88(2):
333 - 349.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Mao, R. P. Yasuda, H. Fan, B. B. Wolfe, and K. J. Kellar
Heterogeneity of Nicotinic Cholinergic Receptors in Rat Superior Cervical and Nodose Ganglia
Mol. Pharmacol.,
November 1, 2006;
70(5):
1693 - 1699.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Xu, M. M. Scott, and E. S. Deneris
Shared Long-Range Regulatory Elements Coordinate Expression of a Gene Cluster Encoding Nicotinic Receptor Heteromeric Subtypes
Mol. Cell. Biol.,
August 1, 2006;
26(15):
5636 - 5649.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. B. Ficklin, S. Zhao, and G. Feng
Ubiquilin-1 Regulates Nicotine-induced Up-regulation of Neuronal Nicotinic Acetylcholine Receptors
J. Biol. Chem.,
October 7, 2005;
280(40):
34088 - 34095.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Rassadi, A. Krishnaswamy, B. Pie, R. McConnell, M. H. Jacob, and E. Cooper
A Null Mutation for the {alpha}3 Nicotinic Acetylcholine (ACh) Receptor Gene Abolishes Fast Synaptic Activity in Sympathetic Ganglia and Reveals That ACh Output from Developing Preganglionic Terminals Is Regulated in an Activity-Dependent Retrograde Manner
J. Neurosci.,
September 14, 2005;
25(37):
8555 - 8566.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Wang, A. Orr-Urtreger, J. Chapman, R. Rabinowitz, and A. D. Korczyn
Deficiency of Nicotinic Acetylcholine Receptor beta 4 Subunit Causes Autonomic Cardiac and Intestinal Dysfunction
Mol. Pharmacol.,
March 1, 2003;
63(3):
574 - 580.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Francis and E. S. Deneris
Retinal Neuron Activity of ETS Domain-binding Sites in a Nicotinic Acetylcholine Receptor Gene Cluster Enhancer
J. Biol. Chem.,
February 15, 2002;
277(8):
6511 - 6519.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. P. Haghighi and E. Cooper
A Molecular Link between Inward Rectification and Calcium Permeability of Neuronal Nicotinic Acetylcholine alpha 3beta 4 and alpha 4beta 2 Receptors
J. Neurosci.,
January 15, 2000;
20(2):
529 - 541.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Xu, A. Orr-Urtreger, F. Nigro, S. Gelber, C. B. Sutcliffe, D. Armstrong, J. W. Patrick, L. W. Role, A. L. Beaudet, and M. De Biasi
Multiorgan Autonomic Dysfunction in Mice Lacking the beta 2 and the beta 4 Subunits of Neuronal Nicotinic Acetylcholine Receptors
J. Neurosci.,
November 1, 1999;
19(21):
9298 - 9305.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Xiao, E. L. Meyer, J. M. Thompson, A. Surin, J. Wroblewski, and K. J. Kellar
Rat alpha 3/beta 4 Subtype of Neuronal Nicotinic Acetylcholine Receptor Stably Expressed in a Transfected Cell Line: Pharmacology of Ligand Binding and Function
Mol. Pharmacol.,
August 1, 1998;
54(2):
322 - 333.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. P. Haghighi and E. Cooper
Neuronal Nicotinic Acetylcholine Receptors Are Blocked by Intracellular Spermine in a Voltage-Dependent Manner
J. Neurosci.,
June 1, 1998;
18(11):
4050 - 4062.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Feng, J. H. Steinbach, and J. R. Sanes
Rapsyn Clusters Neuronal Acetylcholine Receptors But Is Inessential for Formation of an Interneuronal Cholinergic Synapse
J. Neurosci.,
June 1, 1998;
18(11):
4166 - 4176.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. G. Conroy and D. K. Berg
Nicotinic Receptor Subtypes in the Developing Chick Brain: Appearance of a Species Containing the alpha 4, beta 2, and alpha 5 Gene Products
Mol. Pharmacol.,
March 1, 1998;
53(3):
392 - 401.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Rosenberg, B. Pie, and E. Cooper
Developing Neonatal Rat Sympathetic and Sensory Neurons Differ in Their Regulation of 5-HT3 Receptor Expression
J. Neurosci.,
September 1, 1997;
17(17):
6629 - 6638.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. M. Blumenthal, W. G. Conroy, S. J. Romano, P. D. Kassner, and D. K. Berg
Detection of Functional Nicotinic Receptors Blocked by alpha -Bungarotoxin on PC12 Cells and Dependence of Their Expression on Post-Translational Events
J. Neurosci.,
August 15, 1997;
17(16):
6094 - 6104.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. I. Dávila-García, J. L. Musachio, D. C. Perry, Y. Xiao, A. Horti, E. D. London, R. F. Dannals, and K. J. Kellar
[125I]IPH, an Epibatidine Analog, Binds with High Affinity to Neuronal Nicotinic Cholinergic Receptors
J. Pharmacol. Exp. Ther.,
July 1, 1997;
282(1):
445 - 451.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. McDonough and E. Deneris
beta 43': An Enhancer Displaying Neural-Restricted Activity Is Located in the 3'-Untranslated Exon of the Rat Nicotinic Acetylcholine Receptor beta 4 Gene
J. Neurosci.,
April 1, 1997;
17(7):
2273 - 2283.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Gault and R. E. Siegel
Expression of the GABAA Receptor delta Subunit Is Selectively Modulated by Depolarization in Cultured Rat Cerebellar Granule Neurons
J. Neurosci.,
April 1, 1997;
17(7):
2391 - 2399.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Rogers, L. M. Colquhoun, J. W. Patrick, and J. A. Dani
Calcium Flux Through Predominantly Independent Purinergic ATP and Nicotinic Acetylcholine Receptors
J Neurophysiol,
March 1, 1997;
77(3):
1407 - 1417.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-D. Liang and E. S. Vizi
Positive Feedback Modulation of Acetylcholine Release from Isolated Rat Superior Cervical Ganglion
J. Pharmacol. Exp. Ther.,
February 1, 1997;
280(2):
650 - 655.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. S. Levey and M. H. Jacob
Changes in the Regulatory Effects of Cell-Cell Interactions on Neuronal AChR Subunit Transcript Levels after Synapse Formation
J. Neurosci.,
November 1, 1996;
16(21):
6878 - 6885.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Yang, D. Fyodorov, and E. S. Deneris
Transcriptional Analysis of Acetylcholine Receptor [IMAGE]3 Gene Promoter Motifs That Bind Sp1 and AP2
J. Biol. Chem.,
April 14, 1995;
270(15):
8514 - 8520.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. G. Conroy and D. K. Berg
Neurons Can Maintain Multiple Classes of Nicotinic Acetylcholine Receptors Distinguished by Different Subunit Compositions
J. Biol. Chem.,
March 3, 1995;
270(9):
4424 - 4431.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. McDonough, N. Francis, T. Miller, and E. S. Deneris
Regulation of Transcription in the Neuronal Nicotinic Receptor Subunit Gene Cluster by a Neuron-selective Enhancer and ETS Domain Factors
J. Biol. Chem.,
September 8, 2000;
275(37):
28962 - 28970.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. G. Wheeler and E. Cooper
Depolarization Strongly Induces Human Cytomegalovirus Major Immediate-Early Promoter/Enhancer Activity in Neurons
J. Biol. Chem.,
August 17, 2001;
276(34):
31978 - 31985.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
