 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 14, 4915-4926, Copyright © 1994 by Society for Neuroscience
Isolation of clones of rat striatum-specific mRNAs by directional tag PCR subtraction
H Usui, JD Falk, A Dopazo, L de Lecea, MG Erlander and JG Sutcliffe
Department of Molecular Biology, Scripps Research Institute, La Jolla, California 92037.
We report an improved subtractive cDNA cloning procedure, named
"directional tag PCR subtraction," for isolating clones of mRNAs enriched
in a target tissue compared to a second tissue, the driver. In this method,
the target and driver are prepared from directional cDNA libraries
constructed in different vectors, and the target cDNA contains tag
sequences at both its 5' and 3' ends for PCR amplification. This method
avoids several limitations of previous subtraction procedures, and was
demonstrated to be technically easy and efficient. Using the directional
tag PCR subtraction and improved screening procedures, cDNA clones
corresponding to mRNAs expressed in the striatum but not in the cerebellum
of the rat brain were efficiently isolated, including mRNAs encoding
calmodulin-dependent phosphodiesterase, a transcriptional regulatory
protein, and several previously uncharacterized species. Our data suggest
that approximately 1% of the striatal polyA+ RNA mass potentially encoding
more than 300 distinct proteins corresponds to RNA species reduced in
concentration or absent from the cerebellum, of which about one-third are
expressed prominently only in the striatum. This unexpected finding
suggests that the striatum has a unique biochemical character within the
brain, and that characterization of these mRNAs will be important for
understanding the biochemical basis of striatal function.
This article has been cited by other articles:
|
|
|
|
 
S. Subramaniam, K. M. Sixt, R. Barrow, and S. H. Snyder
Rhes, a Striatal Specific Protein, Mediates Mutant-Huntingtin Cytotoxicity
Science,
June 5, 2009;
324(5932):
1327 - 1330.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-W. Chang, C.-W. Tsai, H.-F. Wang, H.-C. Tsai, H.-Y. Chen, T.-F. Tsai, H. Takahashi, H.-Y. Li, M.-J. Fann, C.-W. Yang, et al.
Identification of a developmentally regulated striatum-enriched zinc-finger gene, Nolz-1, in the mammalian brain
PNAS,
February 24, 2004;
101(8):
2613 - 2618.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. de Chaldee, M.-C. Gaillard, N. Bizat, J.-M. Buhler, O. Manzoni, J. Bockaert, P. Hantraye, E. Brouillet, and J.-M. Elalouf
Quantitative Assessment of Transcriptome Differences Between Brain Territories
Genome Res.,
July 1, 2003;
13(7):
1646 - 1653.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. E. Alvarez, J. G. Sutcliffe, and E. A. Thomas
Novel Isoform of Insulin Receptor Substrate p53/p58 Is Generated by Alternative Splicing in the CRIB/SH3-binding Region
J. Biol. Chem.,
June 28, 2002;
277(27):
24728 - 24734.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. G. Sutcliffe
Open-System Approaches to Gene Expression in the CNS
J. Neurosci.,
November 1, 2001;
21(21):
8306 - 8309.
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. K. Vogt, M. Aoki, I. Bottoli, H.-W. Chang, S.-l. Fu, A. Hecht, J. S. Iacovoni, B.-H. Jiang, and U. Kruse
A Random Walk in Oncogene Space: The Quest for Targets
Cell Growth Differ.,
December 1, 1999;
10(12):
777 - 784.
[Full Text]
|
|
|
|
|
|
|
S.-l. Fu, I. Bottoli, M. Goller, and P. K. Vogt
Heparin-binding epidermal growth factor-like growth factor, a v-Jun target gene, induces oncogenic transformation
PNAS,
May 11, 1999;
96(10):
5716 - 5721.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kawasaki, G. M. Springett, S. Toki, J. J. Canales, P. Harlan, J. P. Blumenstiel, E. J. Chen, I. A. Bany, N. Mochizuki, A. Ashbacher, et al.
A Rap guanine nucleotide exchange factor enriched highly in the basal ganglia
PNAS,
October 27, 1998;
95(22):
13278 - 13283.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P Ruiz-Lozano, S. Smith, G Perkins, S. Kubalak, G. Boss, H. Sucov, R. Evans, and K. Chien
Energy deprivation and a deficiency in downstream metabolic target genes during the onset of embryonic heart failure in RXRalpha-/- embryos
Development,
January 2, 1998;
125(3):
533 - 544.
[Abstract]
[PDF]
|
|
|
|
|
|
|
R. W. Williams, R. C. Strom, D. S. Rice, and D. Goldowitz
Genetic and Environmental Control of Variation in Retinal Ganglion Cell Number in Mice
J. Neurosci.,
November 15, 1996;
16(22):
7193 - 7205.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. V. Nguyen, H. Fujioka, A. S. Kang, W. O. Rogers, D. A. Fidock, and A. A. James
Stage-dependent Localization of a Novel Gene Product of the Malaria Parasite, Plasmodium falciparum
J. Biol. Chem.,
July 6, 2001;
276(28):
26724 - 26731.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
