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Journal of Neuroscience, Vol 14, 5160-5169, Copyright © 1994 by Society for Neuroscience
Spatial and temporal expression patterns of two sodium channel genes in Drosophila
CS Hong and B Ganetzky
Laboratory of Genetics, University of Wisconsin, Madison 53706.
Genetic and molecular studies have identified two different sodium channel
genes in Drosophila, para and DSC1. The functional contributions of the
para-encoded channel have been inferred from analysis of mutant phenotypes.
However, no mutations of DSC1 have been identified, so the in vivo
functions of the channel it encodes are not yet known. To learn more about
the possible functions of DSC1 in the Drosophila nervous system compared
with those of para, we have characterized the expression patterns of these
two sodium channel genes at embryonic, larval, pupal, and adult stages by
tissue in situ hybridization, para encodes the predominant type of sodium
channel and is ubiquitously transcribed throughout the CNS and PNS at all
developmental stages. The expression pattern of DSC1 is very different from
para during embryonic and larval stages during which there are very few
DSC1-expressing cells in either the CNS or PNS. Double- labeling studies
suggest that some of these cells are non-neuronal. However, in pupal and
adult stages, para and DSC1 have completely overlapping patterns of
expression in the CNS and retina. In the pupal and adult, PNS expression of
these genes is still distinct because only para transcripts are detected in
wing sensory neurons. The strong and widespread expression of DSC1 in the
CNS of pupae and adults suggests that the DSC1 channels are likely to
provide an important function in neurons during these stages. Since most,
if not all, neurons in the pupal and adult CNS express both para and DSC1,
these two sodium channel genes probably subserve distinct functions within
these cells. Our results provide the background for elucidating the
respective in vivo contributions of para and DSC1 to neuronal excitability
and for dissecting the regulatory mechanisms that underlie their different
patterns of expression.
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