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Journal of Neuroscience, Vol 15, 3128-3137, Copyright © 1995 by Society for Neuroscience
Survival of newly postmitotic motoneurons is transiently independent of exogenous trophic support
C Mettling, A Gouin, M Robinson, H el M'Hamdi, W Camu, E Bloch-Gallego, B Buisson, H Tanaka, AM Davies and CE Henderson
INSERM U.382, Biochimie CNRS-INSERM, Montpellier, France.
We compared the survival requirements of early- and late-born motoneurons
from E5 chicken spinal cord. Density gradient centrifugation followed by
immunopanning using SC1 antibody allowed us to purify two size classes of
motoneuron. Large motoneurons retained by 6.8% metrizamide were shown by
BrdU labeling in ovo to be born on average 1.5 d earlier than the small
motoneurons recovered from the metrizamide pellet. Large motoneurons were
both biochemically and functionally more mature: they expressed higher
levels of choline acetyltransferase and low-affinity neurotrophin receptor,
and had an acute requirement for trophic support from muscle-derived
factors. After 24 hr in culture in basal medium, all early-born motoneurons
died, whereas 60% of late-born motoneurons survived. Small motoneurons can
develop into large motoneurons in ovo, suggesting that they represent a
general transitional stage in motoneuron development. Our results suggest
that a defined period elapses between birth of a motoneuron and its
acquisition of trophic dependence, possibly corresponding to the time
required for target innervation. This property may have important
consequences for the timing and regulation of developmental motoneuron
death.
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