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Journal of Neuroscience, Vol 15, 4533-4544, Copyright © 1995 by Society for Neuroscience
Dopamine-modulated potassium channels on rat striatal neurons: specific activation and cellular expression
GJ Greif, YJ Lin, JC Liu and JE Freedman
Department of Pharmaceutical Sciences, Northeastern University, Boston, Massachusetts 02115, USA.
We have used cell-attached patch-clamp electrophysiology to characterize
the activation and distribution of an 85 pS K+ channel on freshly
dissociated rat striatal (caudate-putamen) neurons. In recordings from 643
cells, openings of this channel showed an absolute dependence on the
presence of dopamine or the D2-like dopamine receptor agonist quinpirole in
the cell-attached patch pipette, but were never seen when the D2 antagonist
domperidone was applied along with quinpirole, or in the absence of drug.
This channel displayed inward rectification at depolarized membrane
potentials, but its activation was otherwise voltage insensitive. It was
largely restricted to a subset of dissociated cells with diameters > or
= 10 microns, with channel openings seen in about 25% of patches. When
present, there were typically multiple channels per patch. Cells of this
size were immunocytochemically stained for neuron-specific enolase but not
glial fibrillary acidic protein; about 40% were also labeled for
gamma-amino butyric acid (GABA) and about 60% for NADPH diaphorase, with
GABAergic cells displaying a shape most similar to that of cells expressing
the channel. A large number of distinct types of other channels were also
present, comprising inwardly rectifying channels of 5-35 pS conductance and
voltage-activated channels of 100-250 pS, but the frequencies of occurrence
and fractional open times of these channels were independent of the
presence or absence of dopaminergic agonists. Thus, the 85 pS K+ channel
uniquely requires activation by a D2-like dopamine receptor on rat striatal
neurons, and is selectively expressed by a subset of these cells, which are
most likely to be GABAergic neurons.
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