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Journal of Neuroscience, Vol 15, 4572-4579, Copyright © 1995 by Society for Neuroscience
Kinase and phosphatase activities intimately associated with a reconstituted calcium-dependent potassium channel
PH Reinhart and IB Levitan
Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Type-2 calcium-dependent potassium (KCa) channels from mammalian brain,
reconstituted into planar phospholipid bilayers, are modulated by ATP or
ATP analogs via an endogenous protein kinase activity intimately associated
with the channel (Chung et al., 1991). We show here that the endogenous
protein kinase activity is protein kinase C (PKC)-like because (1)
modulation by ATP can be mimicked by exogenous PKC, and (2) the effects of
ATP can be blocked by PKC(19-36), a specific peptide inhibitor of PKC.
Furthermore, adding the PKC inhibitor peptide after the addition of ATP
reverses the modulation produced by ATP, suggesting that there is a
phosphoprotein phosphatase activity closely associated with type-2 KCa
channels. Consistent with this idea is the finding that microcystin, a
non-specific phosphatase inhibitor, enhances the modulation of KCa channel
activity by ATP. Inhibitor-1, a specific protein inhibitor of
phosphoprotein phosphatase-1, also enhances the effect of ATP, suggesting
that the endogenous phosphatase activity is phosphatase-1-like. The results
imply that type-2 KCa channels exist as part of a regulatory complex that
includes a PKC-like protein kinase and a phosphatase-1-like phosphoprotein
phosphatase, both of which participate in the modulation of channel
function.
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|
|
|
|
|
|
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|
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|
|
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|
|
|
|
|
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|
|
|
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