 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 15, 5840-5850, Copyright © 1995 by Society for Neuroscience
Protease nexin-1 and thrombin modulate neuronal Ca2+ homeostasis and sensitivity to glucose deprivation-induced injury
VL Smith-Swintosky, S Zimmer, JW Fenton 2nd and MP Mattson
Sanders-Brown Research Center on Aging, University of Kentucky, Lexington 40536-0230, USA.
Protease nexin-I (PN-1) is a 44 kDa serine proteinase inhibitor that
rapidly inhibits thrombin by forming SDS stable complexes with serine at
the catalytic site of the protease. Levels of both PN-1 and thrombin are
increased in the brain in response to insults such as ischemia, suggesting
roles in neural injury and repair processes. We now report that
PN-1-protected cultured rat hippocampal neurons against glucose
deprivation- induced damage (GDID), and the protection was abolished by
equimolar thrombin. PN-1 reduced resting intracellular free calcium levels
([Ca2+]i) and attenuated the elevation of [Ca2+]i normally associated with
GDID. Thrombin reduced neuronal survival and caused a significant increase
in [Ca2+]i. Submaximally toxic levels of thrombin exacerbated GDID. Calcium
responses to thrombin were attenuated in neurons contacting PN-1
immunoreactive astrocytes. These findings suggest that PN-1 and thrombin
play important roles in modulating neuronal calcium responses, and
vulnerability, to metabolic/excitotoxic insults.
This article has been cited by other articles:
|
|
|
|
 
C. J. Lee, G. Mannaioni, H. Yuan, D. H. Woo, M. B. Gingrich, and S. F. Traynelis
Astrocytic control of synaptic NMDA receptors
J. Physiol.,
June 15, 2007;
581(3):
1057 - 1081.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Lino, S. Atanasoski, M. Kvajo, B. Fayard, E. Moreno, H. R. Brenner, U. Suter, and D. Monard
Mice Lacking Protease Nexin-1 Show Delayed Structural and Functional Recovery after Sciatic Nerve Crush
J. Neurosci.,
April 4, 2007;
27(14):
3677 - 3685.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Giau, J. Carrette, J. Bockaert, and V. Homburger
Constitutive Secretion of Protease Nexin-1 by Glial Cells and Its Regulation by G-Protein-Coupled Receptors
J. Neurosci.,
September 28, 2005;
25(39):
8995 - 9004.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Stokely, S. T. Brady, and T. Yorio
Effects of Endothelin-1 on Components of Anterograde Axonal Transport in Optic Nerve
Invest. Ophthalmol. Vis. Sci.,
October 1, 2002;
43(10):
3223 - 3230.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Meins, P. Piosik, N. Schaeren-Wiemers, S. Franzoni, E. Troncoso, J. Z. Kiss, C. Brosamle, M. E. Schwab, Z. Molnar, and D. Monard
Progressive Neuronal and Motor Dysfunction in Mice Overexpressing the Serine Protease Inhibitor Protease Nexin-1 in Postmitotic Neurons
J. Neurosci.,
November 15, 2001;
21(22):
8830 - 8841.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Carhuapoma, P. Y. Wang, N. J. Beauchamp, P. M. Keyl, D. F. Hanley, and P. B. Barker
Diffusion-Weighted MRI and Proton MR Spectroscopic Imaging in the Study of Secondary Neuronal Injury After Intracerebral Hemorrhage
Stroke,
March 1, 2000;
31(3):
726 - 732.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. U Corvera, O. Dery, K. McConalogue, P. Gamp, M. Thoma, B. Al-Ani, G. H Caughey, M. D Hollenberg, and N. W Bunnett
Thrombin and mast cell tryptase regulate guinea-pig myenteric neurons through proteinase-activated receptors-1 and -2
J. Physiol.,
June 15, 1999;
517(3):
741 - 756.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. J. Davies, B. S. Pickard, M. Steel, R. G. M. Morris, and R. Lathe
Serine Proteases in Rodent Hippocampus
J. Biol. Chem.,
September 4, 1998;
273(36):
23004 - 23011.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Monsonego, T. Mizrahi, S. Eitan, G. Moalem, H. Bárdos, R. Ádány, and M. Schwartz
Factor XIIIa as a nerve-associated transglutaminase
FASEB J,
September 1, 1998;
12(12):
1163 - 1171.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
V. L. Turgeon, E. D. Lloyd, S. Wang, B. W. Festoff, and L. J. Houenou
Thrombin Perturbs Neurite Outgrowth and Induces Apoptotic Cell Death in Enriched Chick Spinal Motoneuron Cultures through Caspase Activation
J. Neurosci.,
September 1, 1998;
18(17):
6882 - 6891.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. M. Donovan and D. D. Cunningham
Signaling Pathways Involved in Thrombin-induced Cell Protection
J. Biol. Chem.,
May 22, 1998;
273(21):
12746 - 12752.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. A. Scarisbrick, M. D. Towner, and P. J. Isackson
Nervous System-Specific Expression of a Novel Serine Protease: Regulation in the Adult Rat Spinal Cord by Excitotoxic Injury
J. Neurosci.,
November 1, 1997;
17(21):
8156 - 8168.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. C. Guttridge, A. Lau, L. Tran, and D. D. Cunningham
Thrombin Causes a Marked Delay in Skeletal Myogenesis That Correlates with the Delayed Expression of Myogenin and p21CIP1/WAF1
J. Biol. Chem.,
September 26, 1997;
272(39):
24117 - 24120.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. M. Donovan, C. J. Pike, C. W. Cotman, and D. D. Cunningham
Thrombin Induces Apoptosis in Cultured Neurons and Astrocytes via a Pathway Requiring Tyrosine Kinase and RhoA Activities
J. Neurosci.,
July 15, 1997;
17(14):
5316 - 5326.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Luthi, H. v. d. Putten, F. M. Botteri, I. M. Mansuy, M. Meins, U. Frey, G. Sansig, C. Portet, M. Schmutz, M. Schroder, et al.
Endogenous Serine Protease Inhibitor Modulates Epileptic Activity and Hippocampal Long-Term Potentiation
J. Neurosci.,
June 15, 1997;
17(12):
4688 - 4699.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. Tsirka, A. D. Rogove, T. H. Bugge, J. L. Degen, and S. Strickland
An Extracellular Proteolytic Cascade Promotes Neuronal Degeneration in the Mouse Hippocampus
J. Neurosci.,
January 15, 1997;
17(2):
543 - 552.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. S. Pai, V. B. Mahajan, A. Lau, and D. D. Cunningham
Thrombin Receptor Signaling to Cytoskeleton Requires Hsp90
J. Biol. Chem.,
August 24, 2001;
276(35):
32642 - 32647.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Striggow, M. Riek, J. Breder, P. Henrich-Noack, K. G. Reymann, and G. Reiser
The protease thrombin is an endogenous mediator of hippocampal neuroprotection against ischemia at low concentrations but causes degeneration at high concentrations
PNAS,
February 29, 2000;
97(5):
2264 - 2269.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
