 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 16, 283-297, Copyright © 1996 by Society for Neuroscience
Laminar compartmentalization of GABAA-receptor subtypes in the spinal cord: an immunohistochemical study
S Bohlhalter, O Weinmann, H Mohler and JM Fritschy
Institute of Pharmacology, University of Zurich, Switzerland.
To assess the significance of GABAA-receptor heterogeneity, which is based
on a family of at least 15 subunits, the cellular localization and subunit
composition of GABAA-receptor subtypes were analyzed immunohistochemically
in the rat spinal cord. The distribution of subunits alpha 1, alpha 2,
alpha 3, alpha 5, beta 2,3, and gamma 2 was investigated with
subunit-specific antibodies, and their colocalization within individual
neurons was visualized by double-immunofluorescence staining. The results
reveal a widespread expression of the subunits, alpha 3, beta 2,3, and
gamma 2 in the spinal cord, whereas the three other alpha subunits
displayed a more restricted, lamina-specific distribution. The alpha 1 and
alpha 5 subunits were most abundant in the intermediate zone, whereas the
alpha 2 subunit was predominant in the superficial layers of the dorsal
horn and in somatic and preganglionic motoneurons. From colocalization
studies, seven subunit combinations could be identified (alpha 3/beta
2,3/gamma 2; alpha 2/beta 2,3/gamma 2; alpha 1/beta 2,3/gamma 2; alpha
5/beta 2,3/gamma 2; alpha 1/alpha 5/beta 2,3/gamma 2; alpha 2/gamma 2;
alpha 2/alpha 5/gamma 2) that correspond presumably to distinct receptor
subtypes. Although most neurons expressed the subunit triplet alpha x/beta
2,3/gamma 2, the beta 2,3 subunits could not be detected in motoneurons
that may thus possess "atypical" receptor subtypes (alpha 2/gamma 2 and
alpha 2/alpha 5/gamma 2). ON the subcellular level, aggregates of
immunoreactivity, suggestive of postsynaptic GABAA receptors, typically
were seen on the surface of neuronal somata and proximal dendrites. In
addition, an intense diffuse staining was observed in laminae I--III for
the subunits alpha 2, alpha 3, beta 2,3, and gamma 2, presumably localized
on primary afferent terminals. The localization of GABAA- receptor subtypes
in distinct laminar compartments of the spinal cord suggests that
GABAA-receptor heterogeneity is of relevance for the modulation of sensory
inputs, nociception, and motor control at segmental levels.
This article has been cited by other articles:
|
|
|
|
 
G. Munro, J. A. Lopez-Garcia, I. Rivera-Arconada, H. K. Erichsen, E. O. Nielsen, J. S. Larsen, P. K. Ahring, and N. R. Mirza
Comparison of the Novel Subtype-Selective GABAA Receptor-Positive Allosteric Modulator NS11394 [3'-[5-(1-Hydroxy-1-methyl-ethyl)-benzoimidazol-1-yl]-biphenyl-2-carbonitrile] with Diazepam, Zolpidem, Bretazenil, and Gaboxadol in Rat Models of Inflammatory and Neuropathic Pain
J. Pharmacol. Exp. Ther.,
December 1, 2008;
327(3):
969 - 981.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Wang, E. Spary, J. Deuchars, and S. A. Deuchars
Tonic GABAergic Inhibition of Sympathetic Preganglionic Neurons: A Novel Substrate for Sympathetic Control
J. Neurosci.,
November 19, 2008;
28(47):
12445 - 12452.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. Mitchell, L. J. Gentet, J. Dempster, and D. Belelli
GABAA and glycine receptor-mediated transmission in rat lamina II neurones: relevance to the analgesic actions of neuroactive steroids
J. Physiol.,
September 15, 2007;
583(3):
1021 - 1040.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Grasshoff, R. Jurd, U. Rudolph, and B. Antkowiak
Modulation of Presynaptic beta3-Containing GABAA Receptors Limits the Immobilizing Actions of GABAergic Anesthetics
Mol. Pharmacol.,
September 1, 2007;
72(3):
780 - 787.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Radel, R. L. Vallejo, N. Iwata, R. Aragon, J. C. Long, M. Virkkunen, and D. Goldman
Haplotype-Based Localization of an Alcohol Dependence Gene to the 5q34 {gamma}-Aminobutyric Acid Type A Gene Cluster
Arch Gen Psychiatry,
January 1, 2005;
62(1):
47 - 55.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. K. Sullivan, R. E. Petroski, G. Verge, R. S. Gross, A. C. Foster, and D. E. Grigoriadis
Characterization of the Interaction of Indiplon, a Novel Pyrazolopyrimidine Sedative-Hypnotic, with the GABAA Receptor
J. Pharmacol. Exp. Ther.,
November 1, 2004;
311(2):
537 - 546.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. L. Yaksh and J. W. Allen
The Use of Intrathecal Midazolam in Humans: A Case Study of Process
Anesth. Analg.,
June 1, 2004;
98(6):
1536 - 1545.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. F. Keller, J.-D. Breton, R. Schlichter, and P. Poisbeau
Production of 5{alpha}-Reduced Neurosteroids Is Developmentally Regulated and Shapes GABAA Miniature IPSCs in Lamina II of the Spinal Cord
J. Neurosci.,
January 28, 2004;
24(4):
907 - 915.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Delgado-Lezama, J. Aguilar, and R. Cueva-Rolon
Synaptic Strength Between Motoneurons and Terminals of the Dorsolateral Funiculus Is Regulated by GABA Receptors in the Turtle Spinal Cord
J Neurophysiol,
January 1, 2004;
91(1):
40 - 47.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. Crestani, R. Keist, J.-M. Fritschy, D. Benke, K. Vogt, L. Prut, H. Bluthmann, H. Mohler, and U. Rudolph
Trace fear conditioning involves hippocampal alpha 5 GABAA receptors
PNAS,
June 25, 2002;
99(13):
8980 - 8985.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Mohler, J. M. Fritschy, and U. Rudolph
A New Benzodiazepine Pharmacology
J. Pharmacol. Exp. Ther.,
January 1, 2002;
300(1):
2 - 8.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. F. Keller, J. A. M. Coull, N. Chery, P. Poisbeau, and Y. De Koninck
Region-Specific Developmental Specialization of GABA-Glycine Cosynapses in Laminas I-II of the Rat Spinal Dorsal Horn
J. Neurosci.,
October 15, 2001;
21(20):
7871 - 7880.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. W. Loomis, H. Khandwala, G. Osmond, and M. P. Hefferan
Coadministration of Intrathecal Strychnine and Bicuculline Effects Synergistic Allodynia in the Rat: An Isobolographic Analysis
J. Pharmacol. Exp. Ther.,
March 1, 2001;
296(3):
756 - 761.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. Crestani, K. Löw, R. Keist, M.-J. Mandelli, H. Möhler, and U. Rudolph
Molecular Targets for the Myorelaxant Action of Diazepam
Mol. Pharmacol.,
March 1, 2001;
59(3):
442 - 445.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. Redecker, H. J. Luhmann, G. Hagemann, J.-M. Fritschy, and O. W. Witte
Differential Downregulation of GABAA Receptor Subunits in Widespread Brain Regions in the Freeze-Lesion Model of Focal Cortical Malformations
J. Neurosci.,
July 1, 2000;
20(13):
5045 - 5053.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Rekling, G. D. Funk, D. A. Bayliss, X.-W. Dong, and J. L. Feldman
Synaptic Control of Motoneuronal Excitability
Physiol Rev,
April 1, 2000;
80(2):
767 - 852.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Chery and Y. De Koninck
Junctional versus Extrajunctional Glycine and GABAA Receptor-Mediated IPSCs in Identified Lamina I Neurons of the Adult Rat Spinal Cord
J. Neurosci.,
September 1, 1999;
19(17):
7342 - 7355.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Loup, O. Weinmann, Y. Yonekawa, A. Aguzzi, H.-G. Wieser, and J.-M. Fritschy
A Highly Sensitive Immunofluorescence Procedure for Analyzing the Subcellular Distribution of GABAA Receptor Subunits in the Human Brain
J. Histochem. Cytochem.,
October 1, 1998;
46(10):
1129 - 1140.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. C. Saxena, T. R. Neelands, and R. L. Macdonald
Contrasting Actions of Lanthanum on Different Recombinant gamma -Aminobutyric Acid Receptor Isoforms Expressed in L929 Fibroblasts
Mol. Pharmacol.,
February 1, 1997;
51(2):
328 - 335.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
.gif?ad=17923&adview=true)
