QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mennerick, S. Articles by Zorumski, C. F. Search for Related Content PubMed PubMed Citation Articles by Mennerick, S. Articles by Zorumski, C. F. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CYCLOLEUCINE GLUTAMIC ACID HYDROCHLORIDE

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 16, 55-64, Copyright © 1996 by Society for Neuroscience

ARTICLE

Components of glial responses to exogenous and synaptic glutamate in rat hippocampal microcultures

S Mennerick, A Benz and CF Zorumski
Department of Psychiatry, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

Although glia are known to be sensitive to exogenously applied neurotransmitter substances, little is known about how glia respond to neuronal activity on the millisecond time scale of individual synaptic events. We have explored the electrophysiological effects of excitatory neuronal signaling on glial cells in rat hippocampal microcultures. Exogenous applications of glutamate analogs to islands of glia revealed a large ionotropic AMPA receptor-mediated current and a smaller current mediated by electrogenic glutamate uptake. Glia demonstrated no evidence of NMDA or metabotropic receptor-mediated currents or membrane conductance changes. Despite the dominant contribution of AMPA receptors to exogenous glutamate applications in glia, AMPA receptor currents contributed only approximately 20% to the response of glia to endogenous glutamate release from solitary excitatory neurons. Electrogenic glutamate uptake contributed strongly to glial responses to neuronal stimulation. In addition, some glia exhibited a residual current is response to neuronal stimulation that was not attributable to calcium-dependent transmitter release or to neuronal potassium efflux. These results help provide a context for understanding the role of glial transporters and receptors in nervous system signaling.

This article has been cited by other articles:

				Y. Li, B. Krupa, J.-S. Kang, V. Y. Bolshakov, and G. Liu Glycine Site of NMDA Receptor Serves as a Spatiotemporal Detector of Synaptic Activity Patterns J Neurophysiol, July 1, 2009; 102(1): 578 - 589. [Abstract] [Full Text] [PDF]

				C. L. Palmer, L. Cotton, and J. M. Henley The Molecular Pharmacology and Cell Biology of {alpha}-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid Receptors Pharmacol. Rev., June 1, 2005; 57(2): 253 - 277. [Abstract] [Full Text] [PDF]

				G. Perea and A. Araque Properties of Synaptically Evoked Astrocyte Calcium Signal Reveal Synaptic Information Processing by Astrocytes J. Neurosci., March 2, 2005; 25(9): 2192 - 2203. [Abstract] [Full Text] [PDF]

				A. Bordey and H. Sontheimer Modulation of Glutamatergic Transmission by Bergmann Glial Cells in Rat Cerebellum In Situ J Neurophysiol, February 1, 2003; 89(2): 979 - 988. [Abstract] [Full Text] [PDF]

				A. Araque, E. D. Martin, G. Perea, J. I. Arellano, and W. Buno Synaptically Released Acetylcholine Evokes Ca2+ Elevations in Astrocytes in Hippocampal Slices J. Neurosci., April 1, 2002; 22(7): 2443 - 2450. [Abstract] [Full Text] [PDF]

				L. S. Overstreet, G. A. Kinney, Y.-B. Liu, D. Billups, and N. T. Slater Glutamate Transporters Contribute to the Time Course of Synaptic Transmission in Cerebellar Granule Cells J. Neurosci., November 1, 1999; 19(21): 9663 - 9673. [Abstract] [Full Text] [PDF]

				R. Ventura and K. M. Harris Three-Dimensional Relationships between Hippocampal Synapses and Astrocytes J. Neurosci., August 15, 1999; 19(16): 6897 - 6906. [Abstract] [Full Text] [PDF]

				D. Kalman, S. N. Gomperts, S. Hardy, M. Kitamura, and J. M. Bishop Ras Family GTPases Control Growth of Astrocyte Processes Mol. Biol. Cell, May 1, 1999; 10(5): 1665 - 1683. [Abstract] [Full Text]

				D. J. Linden Synaptically Evoked Glutamate Transport Currents May Be Used To Detect the Expression of Long-Term Potentiation in Cerebellar Culture J Neurophysiol, June 1, 1998; 79(6): 3151 - 3156. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help