WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (57)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Henkel, A. W.
Right arrow Articles by Betz, W. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Henkel, A. W.
Right arrow Articles by Betz, W. J.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH

 Previous Article  |  Next Article 

Volume 16, Number 12, Issue of June 15, 1996 pp. 3960-3967
Copyright ©1996 Society for Neuroscience

Synaptic Vesicle Movements Monitored by Fluorescence Recovery after Photobleaching in Nerve Terminals Stained with FM1-43

Received Feb. 5, 1996; revised March 8, 1996; accepted March 12, 1996.

A. W. Henkel1, L. L. Simpson2, R. M. A. P. Ridge3, and W. J. Betz1

1 Department of Physiology, University of Colorado Medical School, Denver, Colorado 80262, 2 Departments of Medicine and Pharmacology, Jefferson Medical College, Philadelphia, Pennsylvania 19107-6799, and 3 Department of Physiology, Bristol University School of Medical Sciences, Bristol BS8 1TD, United Kingdom

We used the fluorescence recovery after photobleaching technique to monitor movements of synaptic vesicles in top views of living frog motor nerve terminals that had been prestained with the fluorescent dye FM1-43. In each experiment, a small portion of a single stained vesicle cluster was bleached with a laser and monitored subsequently for signs of recovery as neighboring, unbleached vesicles moved into the bleached region. In resting terminals, little or no recovery from photobleaching occurred. Repetitive nerve stimulation, which caused all fluorescent spots to grow dim as dye was released from exocytosing vesicles, did not promote recovery from photobleaching. Pretreatment with botulinum toxin (type A, C, or D) blocked exocytosis and destaining, but intense nerve stimulation still did not cause significant recovery in bleached regions. These results suggest that lateral movements of synaptic vesicles are restricted severely in both resting and stimulated nerve terminals.

We tested for laser-induced photodamage in several ways. Bleached regions could be restained fully with FM1-43, and these restained regions could be destained fully by nerve stimulation. Partially bleached regions could be destained, although the rate of destaining was lower than normal. Brisk recovery from photobleaching occurred after treatment with okadaic acid, which disrupts synaptic vesicle clusters and causes vesicles to spread throughout the nerve terminal. These results suggest that vesicle translocation and recycling machinery was intact in photobleached regions.

Key words: photobleach recovery; FPR; FRAP; synaptic vesicles; okadaic acid; FM1-43; exocytosis




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. M. Brittain, A. D. Piekarz, Y. Wang, T. Kondo, T. R. Cummins, and R. Khanna
An Atypical Role for Collapsin Response Mediator Protein 2 (CRMP-2) in Neurotransmitter Release via Interaction with Presynaptic Voltage-gated Calcium Channels
J. Biol. Chem., November 6, 2009; 284(45): 31375 - 31390.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
V. Westphal, S. O. Rizzoli, M. A. Lauterbach, D. Kamin, R. Jahn, and S. W. Hell
Video-Rate Far-Field Optical Nanoscopy Dissects Synaptic Vesicle Movement
Science, April 11, 2008; 320(5873): 246 - 249.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. LoGiudice, P. Sterling, and G. Matthews
Mobility and Turnover of Vesicles at the Synaptic Ribbon
J. Neurosci., March 19, 2008; 28(12): 3150 - 3158.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. A. Gaffield and W. J. Betz
Synaptic Vesicle Mobility in Mouse Motor Nerve Terminals with and without Synapsin
J. Neurosci., December 12, 2007; 27(50): 13691 - 13700.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
P. Nunes, N. Haines, V. Kuppuswamy, D. J. Fleet, and B. A. Stewart
Synaptic Vesicle Mobility and Presynaptic F-Actin Are Disrupted in a N-ethylmaleimide-sensitive Factor Allele of Drosophila
Mol. Biol. Cell, November 1, 2006; 17(11): 4709 - 4719.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D. L. Fortin, V. M. Nemani, S. M. Voglmaier, M. D. Anthony, T. A. Ryan, and R. H. Edwards
Neural Activity Controls the Synaptic Accumulation of {alpha}-Synuclein
J. Neurosci., November 23, 2005; 25(47): 10913 - 10921.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
E. A. Lemke and J. Klingauf
Single Synaptic Vesicle Tracking in Individual Hippocampal Boutons at Rest and during Synaptic Activity
J. Neurosci., November 23, 2005; 25(47): 11034 - 11044.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. A. Silverman, S. Johnson, D. Gurkins, M. Farmer, J. E. Lochner, P. Rosa, and B. A. Scalettar
Mechanisms of Transport and Exocytosis of Dense-Core Granules Containing Tissue Plasminogen Activator in Developing Hippocampal Neurons
J. Neurosci., March 23, 2005; 25(12): 3095 - 3106.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
Y.-F. Xu, D. Autio, M. B. Rheuben, and W. D. Atchison
Impairment of Synaptic Vesicle Exocytosis and Recycling During Neuromuscular Weakness Produced in Mice by 2,4-Dithiobiuret
J Neurophysiol, December 1, 2002; 88(6): 3243 - 3258.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. A. Rizzo, M. A. Magnuson, P. F. Drain, and D. W. Piston
A Functional Link between Glucokinase Binding to Insulin Granules and Conformational Alterations in Response to Glucose and Insulin
J. Biol. Chem., September 6, 2002; 277(37): 34168 - 34175.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
P. Congar and L.-E. Trudeau
Perturbation of synaptic vesicle delivery during neurotransmitter release triggered independently of calcium influx
J. Physiol., August 1, 2002; 542(3): 779 - 793.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. Dunaevsky and E. A. Connor
F-Actin Is Concentrated in Nonrelease Domains at Frog Neuromuscular Junctions
J. Neurosci., August 15, 2000; 20(16): 6007 - 6012.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
B. Reid, C. R. Slater, and G. S. Bewick
Synaptic Vesicle Dynamics in Rat Fast and Slow Motor Nerve Terminals
J. Neurosci., April 1, 1999; 19(7): 2511 - 2521.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. de Paiva, F. A. Meunier, J. Molgo, K. R. Aoki, and J. O. Dolly
Functional repair of motor endplates after botulinum neurotoxin type A poisoning: Biphasic switch of synaptic activity between nerve sprouts and their parent terminals
PNAS, March 16, 1999; 96(6): 3200 - 3205.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
P. A. Quigley, M. Msghina, C. K. Govind, and H. L. Atwood
Visible Evidence for Differences in Synaptic Effectiveness With Activity-Dependent Vesicular Uptake and Release of FM1-43
J Neurophysiol, January 1, 1999; 81(1): 356 - 370.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
R. Prekeris and D. M. Terrian
Brain Myosin V Is a Synaptic Vesicle-associated Motor Protein: Evidence for a Ca2+-dependent Interaction with the Synaptobrevin-Synaptophysin Complex
J. Cell Biol., June 30, 1997; 137(7): 1589 - 1601.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. Kraszewski, L. Daniell, O. Mundigl, and P. De Camilli
Mobility of Synaptic Vesicles in Nerve Endings Monitored by Recovery from Photobleaching of Synaptic Vesicle-Associated Fluorescence
J. Neurosci., October 1, 1996; 16(19): 5905 - 5913.
[Abstract] [Full Text] [PDF]


-
-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2009 by Society for Neuroscience ONLINE ISSN: 1529-2401
-