 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
Volume 16, Number 13, Issue of July 1, 1996 pp. 4059-4068
Copyright ©1996 Society for NeuroscienceRegulation of Dendritic Spine Density in Cultured Rat Hippocampal Neurons by Steroid Hormones
Received Dec. 8, 1995; revised April 2, 1996; accepted April 4, 1996.
Diane D. Murphy1 and Menahem Segal2 1 Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, and 2 Department of Neurobiology, The Weizmann Institute, Rehovot 76100, Israel
The effects of gonadal steroid hormones on dendritic spines were studied in hippocampal neurons that were dissociated and grown in culture for 2-3 weeks. Exposure to estradiol caused up to a twofold increase in dendritic spine density in these neurons. The effect of estradiol was stereospecific and blocked by the steroid antagonist tamoxifen. The estradiol-induced rise in spine density was blocked by the NMDA antagonist APV, but not by the AMPA/KA antagonist DNQX. The estradiol-induced rise in spine density was blocked by the serine/threonine kinase inhibitor H7, but not by the tyrosine kinase inhibitor genestein, and was partially mimicked by PMA, an activator of protein kinase C. Estradiol also caused an increase in the fluorescence intensity of synaptophysin-immunoreactive terminals, corresponding to presynaptic boutons. Finally, estradiol caused a rise in [Ca]i reactivity of the cultured neurons to topical application of glutamate. These studies are the first to examine receptor and second messenger regulation of dendritic spines, and they illustrate the viability of cultured neurons as a powerful test system to address issues related to the regulation of dendritic spine maturation.
Key words: dendritic spines; estradiol; culture; hippocampal neurons; calcium; plasticity
This article has been cited by other articles:
G. Barreto, M. Santos-Galindo, Y. Diz-Chaves, O. Pernia, P. Carrero, I. Azcoitia, and L. M. Garcia-Segura
Selective Estrogen Receptor Modulators Decrease Reactive Astrogliosis in the Injured Brain: Effects of Aging and Prolonged Depletion of Ovarian Hormones
Endocrinology, November 1, 2009; 150(11): 5010 - 5015.
[Abstract] [Full Text] [PDF]
J.-R. Chen, Y.-T. Yan, T.-J. Wang, L.-J. Chen, Y.-J. Wang, and G.-F. Tseng
Gonadal Hormones Modulate the Dendritic Spine Densities of Primary Cortical Pyramidal Neurons in Adult Female Rat
Cereb Cortex, November 1, 2009; 19(11): 2719 - 2727.
[Abstract] [Full Text] [PDF]
J. Choi, C. M. Ha, E. J. Choi, C. S. Jeong, J. W. Park, J.-H. Baik, J.-Y. Park, M. E. Costa, S. R. Ojeda, and B. J. Lee
Kinesin Superfamily-Associated Protein 3 Is Preferentially Expressed in Glutamatergic Neurons and Contributes to the Excitatory Control of Female Puberty
Endocrinology, December 1, 2008; 149(12): 6146 - 6156.
[Abstract] [Full Text] [PDF]
D. P. Srivastava, K. M. Woolfrey, K. A. Jones, C. Y. Shum, L. L. Lash, G. T. Swanson, and P. Penzes
Rapid enhancement of two-step wiring plasticity by estrogen and NMDA receptor activity
PNAS, September 23, 2008; 105(38): 14650 - 14655.
[Abstract] [Full Text] [PDF]
K. Sasahara, H. Shikimi, S. Haraguchi, H. Sakamoto, S.-i. Honda, N. Harada, and K. Tsutsui
Mode of Action and Functional Significance of Estrogen-Inducing Dendritic Growth, Spinogenesis, and Synaptogenesis in the Developing Purkinje Cell
J. Neurosci., July 11, 2007; 27(28): 7408 - 7417.
[Abstract] [Full Text] [PDF]
K. B. Jelks, R. Wylie, C. L. Floyd, A. K. McAllister, and P. Wise
Estradiol Targets Synaptic Proteins to Induce Glutamatergic Synapse Formation in Cultured Hippocampal Neurons: Critical Role of Estrogen Receptor-{alpha}
J. Neurosci., June 27, 2007; 27(26): 6903 - 6913.
[Abstract] [Full Text] [PDF]
T. Jover-Mengual, R. S. Zukin, and A. M. Etgen
MAPK Signaling Is Critical to Estradiol Protection of CA1 Neurons in Global Ischemia
Endocrinology, March 1, 2007; 148(3): 1131 - 1143.
[Abstract] [Full Text] [PDF]
N. Vasudevan and D. W. Pfaff
Membrane-Initiated Actions of Estrogens in Neuroendocrinology: Emerging Principles
Endocr. Rev., February 1, 2007; 28(1): 1 - 19.
[Abstract] [Full Text] [PDF]
J. H. Morrison, R. D. Brinton, P. J. Schmidt, and A. C. Gore
Estrogen, Menopause, and the Aging Brain: How Basic Neuroscience Can Inform Hormone Therapy in Women
J. Neurosci., October 11, 2006; 26(41): 10332 - 10348.
[Full Text] [PDF]
J. L. Turgeon, M. C. Carr, P. M. Maki, M. E. Mendelsohn, and P. M. Wise
Complex Actions of Sex Steroids in Adipose Tissue, the Cardiovascular System, and Brain: Insights from Basic Science and Clinical Studies
Endocr. Rev., October 1, 2006; 27(6): 575 - 605.
[Abstract] [Full Text] [PDF]
R. M. Facciolo, M. Madeo, R. Alo, M. Canonaco, and F. Dessi-Fulgheri
Neurobiological Effects of Bisphenol A May Be Mediated by Somatostatin Subtype 3 Receptors in Some Regions of the Developing Rat Brain
Toxicol. Sci., December 1, 2005; 88(2): 477 - 484.
[Abstract] [Full Text] [PDF]
C. C. Smith and L. L. McMahon
Estrogen-Induced Increase in the Magnitude of Long-Term Potentiation Occurs Only When the Ratio of NMDA Transmission to AMPA Transmission Is Increased
J. Neurosci., August 24, 2005; 25(34): 7780 - 7791.
[Abstract] [Full Text] [PDF]
N. R. Miller, T. Jover, H. W. Cohen, R. S. Zukin, and A. M. Etgen
Estrogen Can Act via Estrogen Receptor {alpha} and {beta} to Protect Hippocampal Neurons against Global Ischemia-Induced Cell Death
Endocrinology, July 1, 2005; 146(7): 3070 - 3079.
[Abstract] [Full Text] [PDF]
P. S. Green, K. Bales, S. Paul, and G. Bu
Estrogen Therapy Fails to Alter Amyloid Deposition in the PDAPP Model of Alzheimer's Disease
Endocrinology, June 1, 2005; 146(6): 2774 - 2781.
[Abstract] [Full Text] [PDF]
S. Suzuki, T. Numakawa, K. Shimazu, H. Koshimizu, T. Hara, H. Hatanaka, L. Mei, B. Lu, and M. Kojima
BDNF-induced recruitment of TrkB receptor into neuronal lipid rafts: roles in synaptic modulation
J. Cell Biol., December 20, 2004; 167(6): 1205 - 1215.
[Abstract] [Full Text] [PDF]
O. Kretz, L. Fester, U. Wehrenberg, L. Zhou, S. Brauckmann, S. Zhao, J. Prange-Kiel, T. Naumann, H. Jarry, M. Frotscher, et al.
Hippocampal Synapses Depend on Hippocampal Estrogen Synthesis
J. Neurosci., June 30, 2004; 24(26): 5913 - 5921.
[Abstract] [Full Text] [PDF]
C. Li, W. G. Brake, R. D. Romeo, J. C. Dunlop, M. Gordon, R. Buzescu, A. M. Magarinos, P. B. Allen, P. Greengard, V. Luine, et al.
Estrogen alters hippocampal dendritic spine shape and enhances synaptic protein immunoreactivity and spatial memory in female mice
PNAS, February 17, 2004; 101(7): 2185 - 2190.
[Abstract] [Full Text] [PDF]
R. Dominguez, C. Jalali, and S. de Lacalle
Morphological Effects of Estrogen on Cholinergic Neurons In Vitro Involves Activation of Extracellular Signal-Regulated Kinases
J. Neurosci., January 28, 2004; 24(4): 982 - 990.
[Abstract] [Full Text] [PDF]
M. M. Adams and J. H. Morrison
Estrogen and the Aging Hippocampal Synapse
Cereb Cortex, December 1, 2003; 13(12): 1271 - 1275.
[Abstract] [Full Text] [PDF]
H. Sakamoto, Y. Mezaki, H. Shikimi, K. Ukena, and K. Tsutsui
Dendritic Growth and Spine Formation in Response to Estrogen in the Developing Purkinje Cell
Endocrinology, October 1, 2003; 144(10): 4466 - 4477.
[Abstract] [Full Text] [PDF]
T. Deller, M. Korte, S. Chabanis, A. Drakew, H. Schwegler, G. G. Stefani, A. Zuniga, K. Schwarz, T. Bonhoeffer, R. Zeller, et al.
Synaptopodin-deficient mice lack a spine apparatus and show deficits in synaptic plasticity
PNAS, September 2, 2003; 100(18): 10494 - 10499.
[Abstract] [Full Text] [PDF]
L. Zhao, S. Chen, and R. D. Brinton
An Estrogen Replacement Therapy Containing Nine Synthetic Plant-Based Conjugated Estrogens Promotes Neuronal Survival
Experimental Biology and Medicine, July 1, 2003; 228(7): 823 - 835.
[Abstract] [Full Text] [PDF]
D. Yokomaku, T. Numakawa, Y. Numakawa, S. Suzuki, T. Matsumoto, N. Adachi, C. Nishio, T. Taguchi, and H. Hatanaka
Estrogen Enhances Depolarization-Induced Glutamate Release through Activation of Phosphatidylinositol 3-Kinase and Mitogen-Activated Protein Kinase in Cultured Hippocampal Neurons
Mol. Endocrinol., May 1, 2003; 17(5): 831 - 844.
[Abstract] [Full Text] [PDF]
C. N. Rudick and C. S. Woolley
Selective Estrogen Receptor Modulators Regulate Phasic Activation of Hippocampal CA1 Pyramidal Cells by Estrogen
Endocrinology, January 1, 2003; 144(1): 179 - 187.
[Abstract] [Full Text] [PDF]
S. K. Amateau and M. M. McCarthy
A Novel Mechanism of Dendritic Spine Plasticity Involving Estradiol Induction of Prostaglandin-E2
J. Neurosci., October 1, 2002; 22(19): 8586 - 8596.
[Abstract] [Full Text] [PDF]
R. A. DeFazio and S. M. Moenter
Estradiol Feedback Alters Potassium Currents and Firing Properties of Gonadotropin-Releasing Hormone Neurons
Mol. Endocrinol., October 1, 2002; 16(10): 2255 - 2265.
[Abstract] [Full Text] [PDF]
H. M. Fillit
The Role of Hormone Replacement Therapy in the Prevention of Alzheimer Disease
Arch Intern Med, September 23, 2002; 162(17): 1934 - 1942.
[Abstract] [Full Text] [PDF]
L. Zhao, Q. Chen, and R. D. Brinton
Neuroprotective and Neurotrophic Efficacy of Phytoestrogens in Cultured Hippocampal Neurons
Experimental Biology and Medicine, July 1, 2002; 227(7): 509 - 519.
[Abstract] [Full Text] [PDF]
G. W. Huntley, O. Gil, and O. Bozdagi
The Cadherin Family of Cell Adhesion Molecules: Multiple Roles in Synaptic Plasticity
Neuroscientist, June 1, 2002; 8(3): 221 - 233.
[Abstract] [PDF]
T. Jover, H. Tanaka, A. Calderone, K. Oguro, M. V. L. Bennett, A. M. Etgen, and R. S. Zukin
Estrogen Protects against Global Ischemia-Induced Neuronal Death and Prevents Activation of Apoptotic Signaling Cascades in the Hippocampal CA1
J. Neurosci., March 15, 2002; 22(6): 2115 - 2124.
[Abstract] [Full Text] [PDF]
B. McEwen
Estrogen Actions Throughout the Brain
Recent Prog. Horm. Res., January 1, 2002; 57(1): 357 - 384.
[Abstract] [Full Text] [PDF]
B. S. McEwen
Genome and Hormones: Gender Differences in Physiology: Invited Review: Estrogens effects on the brain: multiple sites and molecular mechanisms
J Appl Physiol, December 1, 2001; 91(6): 2785 - 2801.
[Abstract] [Full Text] [PDF]
C. A. Stafstrom-Davis, C. C. Ouimet, J. Feng, P. B. Allen, P. Greengard, and T. A. Houpt
Impaired Conditioned Taste Aversion Learning in Spinophilin Knockout Mice
Learn. Mem., September 1, 2001; 8(5): 272 - 278.
[Abstract] [Full Text] [PDF]
C. N. Rudick and C. S. Woolley
Estrogen Regulates Functional Inhibition of Hippocampal CA1 Pyramidal Cells in the Adult Female Rat
J. Neurosci., September 1, 2001; 21(17): 6532 - 6543.
[Abstract] [Full Text] [PDF]
J. M. Daniel and G. P. Dohanich
Acetylcholine Mediates the Estrogen-Induced Increase in NMDA Receptor Binding in CA1 of the Hippocampus and the Associated Improvement in Working Memory
J. Neurosci., September 1, 2001; 21(17): 6949 - 6956.
[Abstract] [Full Text] [PDF]
E. Korkotian and M. Segal
Regulation of Dendritic Spine Motility in Cultured Hippocampal Neurons
J. Neurosci., August 15, 2001; 21(16): 6115 - 6124.
[Abstract] [Full Text] [PDF]
M. M. Adams, R. A. Shah, W. G. M. Janssen, and J. H. Morrison
Different modes of hippocampal plasticity in response to estrogen in young and aged female rats
PNAS, June 20, 2001; (2001) 141215898.
[Abstract] [Full Text] [PDF]
B. McEwen, K. Akama, S. Alves, W. G. Brake, K. Bulloch, S. Lee, C. Li, G. Yuen, and T. A. Milner
Tracking the estrogen receptor in neurons: Implications for estrogen-induced synapse formation
PNAS, June 19, 2001; 98(13): 7093 - 7100.
[Abstract] [Full Text] [PDF]
R. D. Brinton
Cellular and Molecular Mechanisms of Estrogen Regulation of Memory Function and Neuroprotection Against Alzheimer's Disease: Recent Insights and Remaining Challenges
Learn. Mem., May 1, 2001; 8(3): 121 - 133.
[Abstract] [Full Text] [PDF]
D. B. Dubal and P. M. Wise
Neuroprotective Effects of Estradiol in Middle-Aged Female Rats
Endocrinology, January 1, 2001; 142(1): 43 - 48.
[Abstract] [Full Text] [PDF]
M. Goldin, M. Segal, and E. Avignone
Functional Plasticity Triggers Formation and Pruning of Dendritic Spines in Cultured Hippocampal Networks
J. Neurosci., January 1, 2001; 21(1): 186 - 193.
[Abstract] [Full Text] [PDF]
K. Braun and M. Segal
FMRP Involvement in Formation of Synapses among Cultured Hippocampal Neurons
Cereb Cortex, October 1, 2000; 10(10): 1045 - 1052.
[Abstract] [Full Text] [PDF]
J. Feng, Z. Yan, A. Ferreira, K. Tomizawa, J. A. Liauw, M. Zhuo, P. B. Allen, C. C. Ouimet, and P. Greengard
Spinophilin regulates the formation and function of dendritic spines
PNAS, August 1, 2000; 97(16): 9287 - 9292.
[Abstract] [Full Text] [PDF]
M. J. Hasbani, S. M. Underhill, G. De Erausquin, and M. P. Goldberg
Synapse Loss and Regeneration: A Mechanism for Functional Decline and Recovery after Cerebral Ischemia?
Neuroscientist, April 1, 2000; 6(2): 110 - 119.
[Abstract] [PDF]
L. H. Calizo and L. M. Flanagan-Cato
Estrogen Selectively Regulates Spine Density within the Dendritic Arbor of Rat Ventromedial Hypothalamic Neurons
J. Neurosci., February 15, 2000; 20(4): 1589 - 1596.
[Abstract] [Full Text] [PDF]
J. L. Ross, D. Roeltgen, P. Feuillan, H. Kushner, and G. B. Cutler Jr.
Use of estrogen in young girls with Turner syndrome: Effects on memory
Neurology, January 11, 2000; 54(1): 164 - 164.
[Abstract] [Full Text] [PDF]
C. Patrone, S. Andersson, L. Korhonen, and D. Lindholm
Estrogen receptor-dependent regulation of sensory neuron survival in developing dorsal root ganglion
PNAS, September 14, 1999; 96(19): 10905 - 10910.
[Abstract] [Full Text] [PDF]
P. M. Wise, M. J. Smith, D. B. Dubal, M. E. Wilson, K. M. Krajnak, and K. L. Rosewell
Neuroendocrine Influences and Repercussions of the Menopause
Endocr. Rev., June 1, 1999; 20(3): 243 - 248.
[Abstract] [Full Text]
B. S. McEwen and S. E. Alves
Estrogen Actions in the Central Nervous System
Endocr. Rev., June 1, 1999; 20(3): 279 - 307.
[Abstract] [Full Text]
B. S. McEwen
The Molecular and Neuroanatomical Basis for Estrogen Effects in the Central Nervous System
J. Clin. Endocrinol. Metab., June 1, 1999; 84(6): 1790 - 1797.
[Full Text]
L. D. Pozzo-Miller, T. Inoue, and D. D. Murphy
Estradiol Increases Spine Density and NMDA-Dependent Ca2+ Transients in Spines of CA1 Pyramidal Neurons From Hippocampal Slices
J Neurophysiol, March 1, 1999; 81(3): 1404 - 1411.
[Abstract] [Full Text] [PDF]
M. R. Foy, J. Xu, X. Xie, R. D. Brinton, R. F. Thompson, and T. W. Berger
17beta -Estradiol Enhances NMDA Receptor-Mediated EPSPs and Long-Term Potentiation
J Neurophysiol, February 1, 1999; 81(2): 925 - 929.
[Abstract] [Full Text] [PDF]
R. Kraft, R. B. Levine, and L. L. Restifo
The Steroid Hormone 20-Hydroxyecdysone Enhances Neurite Growth of Drosophila Mushroom Body Neurons Isolated during Metamorphosis
J. Neurosci., November 1, 1998; 18(21): 8886 - 8899.
[Abstract] [Full Text] [PDF]
D. D. Murphy, N. B. Cole, and M. Segal
Brain-derived neurotrophic factor mediates estradiol-induced dendritic spine formation in hippocampal neurons
PNAS, September 15, 1998; 95(19): 11412 - 11417.
[Abstract] [Full Text] [PDF]
E. M. Espreafico, D. E. Coling, V. Tsakraklides, K. Krogh, J. S. Wolenski, G. Kalinec, and B. Kachar
Localization of myosin-V in the centrosome
PNAS, July 21, 1998; 95(15): 8636 - 8641.
[Abstract] [Full Text] [PDF]
D. D. Murphy, N. B. Cole, V. Greenberger, and M. Segal
Estradiol Increases Dendritic Spine Density by Reducing GABA Neurotransmission in Hippocampal Neurons
J. Neurosci., April 1, 1998; 18(7): 2550 - 2559.
[Abstract] [Full Text] [PDF]
G. E. Wood and T. J. Shors
Stress facilitates classical conditioning in males, but impairs classical conditioning in females through activational effects of ovarian hormones
PNAS, March 31, 1998; 95(7): 4066 - 4071.
[Abstract] [Full Text] [PDF]
C. S. Woolley, N. G. Weiland, B. S. McEwen, and P. A. Schwartzkroin
Estradiol Increases the Sensitivity of Hippocampal CA1 Pyramidal Cells to NMDA Receptor-Mediated Synaptic Input: Correlation with Dendritic Spine Density
J. Neurosci., March 1, 1997; 17(5): 1848 - 1859.
[Abstract] [Full Text] [PDF]
D. D. Murphy and M. Segal
Morphological plasticity of dendritic spines in central neurons is mediated by activation of cAMP response element binding protein
PNAS, February 18, 1997; 94(4): 1482 - 1487.
[Abstract] [Full Text] [PDF]
M. M. Adams, R. A. Shah, W. G. M. Janssen, and J. H. Morrison
Different modes of hippocampal plasticity in response to estrogen in young and aged female rats
PNAS, July 3, 2001; 98(14): 8071 - 8076.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|