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Volume 16, Number 14,
Issue of July 15, 1996
pp. 4411-4419
Copyright ©1996 Society for Neuroscience
Upregulation of BDNF mRNA Expression in the Barrel Cortex of
Adult Mice after Sensory Stimulation
Received Feb. 23, 1996; revised April 22, 1996; accepted April 24, 1996.
Nativitat Rocamora1,
Egbert Welker2,
Marta Pascual1, and
Eduardo Soriano1
1 Department of Animal and Plant Cell Biology, Faculty
of Biology, University of Barcelona, 08028 Barcelona, Spain, and
2 Institute of Anatomy, University of Lausanne, 1005 Lausanne, Switzerland
Upregulation of brain-derived neurotrophic factor (BDNF) mRNA
expression by neuronal activity has been reported in cultured
hippocampal cells and in different in vivo excitotoxic
paradigms. The aim of the present study was to determine whether
sensory stimulation of the whisker-to-barrel pathway alters BDNF mRNA
expression in the cortex and, if so, to evaluate the specificity of
this effect. To this end, a set of mystacial whiskers was unilaterally
stimulated by mechanical deflection, and the expression of BDNF mRNA
was analyzed in the barrel cortex by in situ hybridization
(ISH) using a 35S-labeled antisense BDNF
riboprobe and emulsion autoradiography. A clear-cut and specific
upregulation of the BDNF mRNA expression was found at the level of the
somatosensory cortex after the increased peripheral stimulation. In the
barrel cortex of control mice, BDNF mRNA was present in a few cells in
layers II/III and VI, whereas it was almost undetectable in layer IV.
After 6 hr of whisker stimulation, increased levels of BDNF mRNA were
found in layers II to VI of the contralateral barrel cortex. In layer
IV, BDNF upregulation was confined to the barrels corresponding to the
stimulated follicles. ISH combined with immunocytochemistry against the
three calcium-binding proteins parvalbumin, calretinin, and
calbindin-D28K revealed that BDNF mRNA-expressing
cells do not belong to the GABAergic cell population of the barrel
cortex. The present results support a role for BDNF in
activity-dependent modifications of the adult cerebral cortex.
Key words:
in situ hybridization;
immunocytochemistry;
neurotrophins;
GABAergic cells;
activity-dependent
plasticity;
habituation;
somatosensory cortex;
whisker stimulation
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