QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (35) Citing Articles via Google Scholar Google Scholar Articles by Kim, Y.-T. Articles by Wu, C.-F. Search for Related Content PubMed PubMed Citation Articles by Kim, Y.-T. Articles by Wu, C.-F.

 Previous Article  |  Next Article 

Volume 16, Number 18, Issue of September 15, 1996 pp. 5593-5602
Copyright ©1996 Society for Neuroscience

Reduced Growth Cone Motility in Cultured Neurons from Drosophila Memory Mutants with a Defective cAMP Cascade

Received March 19, 1996; revised June 25, 1996; accepted June 26, 1996.

Yun-Taik Kim and Chun-Fang Wu

Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242

Drosophila memory mutants dunce (dnc) and rutabaga (rut) are known to have altered intracellular cAMP levels, nerve terminal growth, and plasticity of synaptic transmission. Because the growth cone is responsible for neurite outgrowth and synaptogenesis, video microscopy was used to examine growth cone morphology and behavior of mutant neurons in larval CNS cultures. We found that growth cone exploratory movement was nearly arrested by both mutations, even though they change cAMP levels in opposite directions. The dnc phenotype could be mimicked by normal neurons when perfused with dibutyryl cAMP (db-cAMP) or forskolin. In contrast, rut growth cones became active when perfused with db-cAMP. Furthermore, motility was also restored by counterbalancing the effects of the two genes in double mutants, indicating that dynamic control of growth cone motility in developing Drosophila neurons requires optimal cAMP levels within an operational range. These findings represent the first demonstration of altered growth cone properties in learning and memory mutants and establish in a natural setting the role of cAMP in growth cone motility and neuronal plasticity.

Key words: dnc; rut; growth cone; lamellipodia; neuronal plasticity; learning; memory; cAMP; Drosophila; CNS; culture

This article has been cited by other articles:

				I-F. Peng, B. A. Berke, Y. Zhu, W.-H. Lee, W. Chen, and C.-F. Wu Temperature-Dependent Developmental Plasticity of Drosophila Neurons: Cell-Autonomous Roles of Membrane Excitability, Ca2+ Influx, and cAMP Signaling J. Neurosci., November 14, 2007; 27(46): 12611 - 12622. [Abstract] [Full Text] [PDF]

				R. Kraft, M. M. Escobar, M. L. Narro, J. L. Kurtis, A. Efrat, K. Barnard, and L. L. Restifo Phenotypes of Drosophila Brain Neurons in Primary Culture Reveal a Role for Fascin in Neurite Shape and Trajectory. J. Neurosci., August 23, 2006; 26(34): 8734 - 8747. [Abstract] [Full Text] [PDF]

				A. Haase and G. Bicker Nitric oxide and cyclic nucleotides are regulators of neuronal migration in an insect embryo Development, September 1, 2003; 130(17): 3977 - 3987. [Abstract] [Full Text] [PDF]

				W. B. Alshuaib and M. V. Mathew Reduced Delayed-Rectifier K+ Current in the Learning Mutant rutabaga Learn. Mem., November 1, 2002; 9(6): 368 - 375. [Abstract] [Full Text] [PDF]

				B. Berke and C.-F. Wu Regional Calcium Regulation within Cultured Drosophila Neurons: Effects of Altered cAMP Metabolism by the Learning Mutations dunce and rutabaga J. Neurosci., June 1, 2002; 22(11): 4437 - 4447. [Abstract] [Full Text] [PDF]

				W. J. Wolfgang, A. Hoskote, I. J. H. Roberts, S. Jackson, and M. Forte Genetic Analysis of the Drosophila Gs{{alpha}} Gene Genetics, July 1, 2001; 158(3): 1189 - 1201. [Abstract] [Full Text] [PDF]

				J. J. Renger, A. Ueda, H. L. Atwood, C. K. Govind, and C.-F. Wu Role of cAMP Cascade in Synaptic Stability and Plasticity: Ultrastructural and Physiological Analyses of Individual Synaptic Boutons in Drosophila Memory Mutants J. Neurosci., June 1, 2000; 20(11): 3980 - 3992. [Abstract] [Full Text] [PDF]

				W.-D. Yao, J. Rusch, M.-m. Poo, and C.-F. Wu Spontaneous Acetylcholine Secretion from Developing Growth Cones of Drosophila Central Neurons in Culture: Effects of cAMP-Pathway Mutations J. Neurosci., April 1, 2000; 20(7): 2626 - 2637. [Abstract] [Full Text] [PDF]

				C. M. Isbister and T. P. O'Connor Filopodial Adhesion Does Not Predict Growth Cone Steering Events In Vivo J. Neurosci., April 1, 1999; 19(7): 2589 - 2600. [Abstract] [Full Text] [PDF]

				Q. Wang and J. Q. Zheng cAMP-Mediated Regulation of Neurotrophin-Induced Collapse of Nerve Growth Cones J. Neurosci., July 1, 1998; 18(13): 4973 - 4984. [Abstract] [Full Text] [PDF]

				M.-L. Zhao and C.-F. Wu Alterations in Frequency Coding and Activity Dependence of Excitability in Cultured Neurons of Drosophila Memory Mutants J. Neurosci., March 15, 1997; 17(6): 2187 - 2199. [Abstract] [Full Text] [PDF]

				A Rajnicek and C McCaig Guidance of CNS growth cones by substratum grooves and ridges: effects of inhibitors of the cytoskeleton, calcium channels and signal transduction pathways J. Cell Sci., January 12, 1997; 110(23): 2915 - 2924. [Abstract] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help