QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (22) Citing Articles via Google Scholar Google Scholar Articles by Castillo, P. E. Articles by Nicoll, R. A. Search for Related Content PubMed PubMed Citation Articles by Castillo, P. E. Articles by Nicoll, R. A.

 Previous Article  |  Next Article 

Volume 16, Number 19, Issue of October 1, 1996 pp. 5942-5950
Copyright ©1996 Society for Neuroscience

Characterizing the Site and Mode of Action of Dynorphin at Hippocampal Mossy Fiber Synapses in the Guinea Pig

Received March 15, 1996; revised July 2, 1996; accepted July 8, 1996.

Pablo E. Castillo, Paul A. Salin, Marc G. Weisskopf, and Roger A. Nicoll

Departments of Cellular and Molecular Pharmacology and Physiology, University of California at San Francisco, San Francisco, California 94143-0450

Extracellular field potential recordings from the CA3 region in guinea pig hippocampal slices were used to study the release and action of dynorphin at the mossy fiber synapse. Dynorphin A(1-17) or U69593 inhibited mossy fiber synaptic responses in preparations in which the CA3 region was surgically isolated from the rest of the hippocampus. This inhibition was completely reversed by the ₁ selective antagonist nor-BNI, thus establishing the presence of functional ₁ receptors in CA3. Inhibitory effects of dynorphin on mossy fiber responses were unaltered in the presence of the N- or P-type Ca²⁺ channel blockers, -CgTx or -Aga IVA, respectively. This indicates that the action of dynorphin is independent of the particular type of Ca²⁺ channel that mediates transmitter release at the mossy fiber terminal. Heterosynaptic inhibition of mossy fiber responses was observed in the presence of nifedipine, -CgTx, or -Aga IVA, indicating that dynorphin release does not depend specifically on L-, N-, or P-type Ca²⁺ channels. The blockade of heterosynaptic inhibition by the membrane-permeant Ca²⁺ chelator EGTA-AM suggests the involvement of a slow Ca²⁺-dependent process in dynorphin release. On the basis of a variety of experimental evidence, we propose that the time course of heterosynaptic inhibition is determined primarily by the time course of clearance of dynorphin in the extracellular space.

Key words: dynorphin; receptors; calcium channels; hippocampus; mossy fibers; opioid

This article has been cited by other articles:

				K. J. Iremonger and J. S. Bains Retrograde Opioid Signaling Regulates Glutamatergic Transmission in the Hypothalamus J. Neurosci., June 3, 2009; 29(22): 7349 - 7358. [Abstract] [Full Text] [PDF]

				B. Pan, C. J. Hillard, and Q.-s. Liu D2 Dopamine Receptor Activation Facilitates Endocannabinoid-Mediated Long-Term Synaptic Depression of GABAergic Synaptic Transmission in Midbrain Dopamine Neurons via cAMP-Protein Kinase A Signaling J. Neurosci., December 24, 2008; 28(52): 14018 - 14030. [Abstract] [Full Text] [PDF]

				Y. Li and A. N. van den Pol Differential Target-Dependent Actions of Coexpressed Inhibitory Dynorphin and Excitatory Hypocretin/Orexin Neuropeptides J. Neurosci., December 13, 2006; 26(50): 13037 - 13047. [Abstract] [Full Text] [PDF]

				G. O. Hjelmstad Interactions Between Asynchronous Release and Short-Term Plasticity in the Nucleus Accumbens Slice J Neurophysiol, March 1, 2006; 95(3): 2020 - 2023. [Abstract] [Full Text] [PDF]

				J. M. Harrison, R. G. Allen, M. J. Pellegrino, J. T. Williams, and O. J. Manzoni Chronic Morphine Treatment Alters Endogenous Opioid Control of Hippocampal Mossy Fiber Synaptic Transmission J Neurophysiol, May 1, 2002; 87(5): 2464 - 2470. [Abstract] [Full Text] [PDF]

				O. Caillard, H. Moreno, B. Schwaller, I. Llano, M. R. Celio, and A. Marty Role of the calcium-binding protein parvalbumin in short-term synaptic plasticity PNAS, November 2, 2000; (2000) 230362997. [Abstract] [Full Text]

				Q. Tang, R. M. Lynch, F. Porreca, and J. Lai Dynorphin A Elicits an Increase in Intracellular Calcium in Cultured Neurons Via a Non-Opioid, Non-NMDA Mechanism J Neurophysiol, May 1, 2000; 83(5): 2610 - 2615. [Abstract] [Full Text] [PDF]

				I. V. Goussakov, K. Fink, C. E. Elger, and H. Beck Metaplasticity of Mossy Fiber Synaptic Transmission Involves Altered Release Probability J. Neurosci., May 1, 2000; 20(9): 3434 - 3441. [Abstract] [Full Text] [PDF]

				S. G. Madamba, P. Schweitzer, and G. R. Siggins Dynorphin Selectively Augments the M-Current in Hippocampal CA1 Neurons by an Opiate Receptor Mechanism J Neurophysiol, October 1, 1999; 82(4): 1768 - 1775. [Abstract] [Full Text] [PDF]

				A. Kapur, M. F. Yeckel, R. Gray, and D. Johnston L-Type Calcium Channels Are Required for One Form of Hippocampal Mossy Fiber LTP J Neurophysiol, April 1, 1998; 79(4): 2181 - 2190. [Abstract] [Full Text] [PDF]

				R. M. Caudle, A. J. Mannes, and M. J. Iadarola GR89,696 Is a Kappa-2 Opioid Receptor Agonist and a Kappa-1 Opioid Receptor Antagonist in the Guinea Pig Hippocampus J. Pharmacol. Exp. Ther., December 1, 1997; 283(3): 1342 - 1349. [Abstract] [Full Text]

				O. Caillard, H. Moreno, B. Schwaller, I. Llano, M. R. Celio, and A. Marty Role of the calcium-binding protein parvalbumin in short-term synaptic plasticity PNAS, November 21, 2000; 97(24): 13372 - 13377. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help