QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (90) Citing Articles via Google Scholar Google Scholar Articles by Pecins-Thompson, M. Articles by Bethea, C. L. Search for Related Content PubMed PubMed Citation Articles by Pecins-Thompson, M. Articles by Bethea, C. L.

 Previous Article  |  Next Article 

Volume 16, Number 21, Issue of November 1, 1996 pp. 7021-7029
Copyright ©1996 Society for Neuroscience

Ovarian Steroid Regulation of Tryptophan Hydroxylase mRNA Expression in Rhesus Macaques

Received May 22, 1996; revised July 9, 1996; accepted Aug. 7, 1996.

Melanie Pecins-Thompson, Nancy A. Brown, Steven G. Kohama, and Cynthia L. Bethea

Divisions of Reproductive Sciences and Neuroscience, Oregon Regional Primate Research Center, Beaverton, Oregon 97006, and Department of Physiology, Oregon Health Sciences University, Portland, Oregon 97201

Progesterone (P) stimulates prolactin secretion through an unknown neural mechanism in estrogen (E)-primed female monkeys. Serotonin is a stimulatory neurotransmitter in prolactin regulation, and this laboratory has shown previously that E induces progestin receptors (PR) in serotonin neurons. Therefore, we questioned whether E and/or E+P increased serotonin neural function. The expression of mRNA for tryptophan hydroxylase (TPH) was examined in ovariectomized (spayed) control, E-treated (28 d), and E+P-treated monkeys (14 d E and 14 d E+P) using in situ hybridization and a 249 bp TPH cRNA probe generated with RT-PCR (n = 5 animals/group). Densitometric analysis of film autoradiographs revealed a ninefold increase in TPH mRNA in E-treated macaques compared to spayed animals (p < 0.05). With supplemental P treatment, TPH mRNA signal was increased fivefold over spayed animals (p < 0.05), but was not significantly different compared to E-treated animals. These results were verified by grain counts from photographic emulsion-coated slides. There were significantly higher single-cell levels of TPH mRNA in serotonergic neurons of the dorsal raphe in E- and E+P-treated groups (p < 0.05). These data indicate that E induces TPH gene expression in nonhuman primates and that the addition of P has little additive effect on TPH gene expression. Thus, the action of P on prolactin secretion is probably not mediated at the level of TPH gene transcription. However, because P increases raphe serotonin content in E-primed rodents, the possibility remains that P may have other actions on post-translational processing or enzyme activity.

Key words: tryptophan hydroxylase mRNA; progesterone; estrogen; serotonin; monkey; dorsal raphe

This article has been cited by other articles:

				E. R. Butelman, S. Rus, D. S. Simpson, A. Wolf, T. E. Prisinzano, and M. J. Kreek The Effects of Herkinorin, the First {micro}-Selective Ligand from a Salvinorin A-Derived Scaffold, in a Neuroendocrine Biomarker Assay in Nonhuman Primates J. Pharmacol. Exp. Ther., October 1, 2008; 327(1): 154 - 160. [Abstract] [Full Text] [PDF]

				A. R. Genazzani, N. Pluchino, S. Luisi, and M. Luisi Estrogen, cognition and female ageing Hum. Reprod. Update, March 1, 2007; 13(2): 175 - 187. [Abstract] [Full Text] [PDF]

				E. R. Butelman, M. Mandau, K. Tidgewell, T. E. Prisinzano, V. Yuferov, and M. J. Kreek Effects of Salvinorin A, a {kappa}-Opioid Hallucinogen, on a Neuroendocrine Biomarker Assay in Nonhuman Primates with High {kappa}-Receptor Homology to Humans J. Pharmacol. Exp. Ther., January 1, 2007; 320(1): 300 - 306. [Abstract] [Full Text] [PDF]

				Z. Amin, T. Canli, and C. N. Epperson Effect of Estrogen-Serotonin Interactions on Mood and Cognition Behav Cogn Neurosci Rev, March 1, 2005; 4(1): 43 - 58. [Abstract] [PDF]

				A. G. Zabka, G. S. Mitchell, E. B. Olson Jr, and M. Behan Selected Contribution: Chronic intermittent hypoxia enhances respiratory long-term facilitation in geriatric female rats J Appl Physiol, December 1, 2003; 95(6): 2614 - 2623. [Abstract] [Full Text]

				W J Cutter, R Norbury, and D G M Murphy Oestrogen, brain function, and neuropsychiatric disorders J. Neurol. Neurosurg. Psychiatry, July 1, 2003; 74(7): 837 - 840. [Full Text] [PDF]

				B. S. McEwen Genome and Hormones: Gender Differences in Physiology: Invited Review: Estrogens effects on the brain: multiple sites and molecular mechanisms J Appl Physiol, December 1, 2001; 91(6): 2785 - 2801. [Abstract] [Full Text] [PDF]

				E. Terasawa and D. L. Fernandez Neurobiological Mechanisms of the Onset of Puberty in Primates Endocr. Rev., February 1, 2001; 22(1): 111 - 151. [Abstract] [Full Text]

				C. Flores, N. Salmaso, S. Cain, D. Rodaros, and J. Stewart Ovariectomy of Adult Rats Leads to Increased Expression of Astrocytic Basic Fibroblast Growth Factor in the Ventral Tegmental Area and in Dopaminergic Projection Regions of the Entorhinal and Prefrontal Cortex J. Neurosci., October 1, 1999; 19(19): 8665 - 8673. [Abstract] [Full Text] [PDF]

				C. N. Epperson, K. L. Wisner, and B. Yamamoto Gonadal Steroids in the Treatment of Mood Disorders Psychosom Med, September 1, 1999; 61(5): 676 - 697. [Abstract] [Full Text] [PDF]

				B. S. McEwen and S. E. Alves Estrogen Actions in the Central Nervous System Endocr. Rev., June 1, 1999; 20(3): 279 - 307. [Abstract] [Full Text]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help