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Volume 16, Number 24,
Issue of December 15, 1996
pp. 8181-8192
Copyright ©1996 Society for Neuroscience
Ultrastructural Localization Suggests that Retinal and Cortical
Inputs Access Different Metabotropic Glutamate Receptors in the Lateral
Geniculate Nucleus
Received June 21, 1996; revised Sept. 27, 1996; accepted Oct. 2, 1996.
Dwayne W. Godwin,
Susan C. Van
Horn,
Alev Eri ir,
Michael Sesma,
Carmelo Romano, and
S.
Murray Sherman
Department of Neurobiology, State University of New York, Stony
Brook, New York 11794-5230
Glutamate has an important neuromodulatory role in synaptic
transmission through metabotropic glutamate receptors (mGluRs) linked
to a variety of G-protein-coupled second messenger pathways. Activation
of these receptors on relay cells in the lateral geniculate nucleus
(LGN) with the agonist
trans-(1S,3R) 1-amino-1,3-cyclopentanedicarboxylic acid produces a
membrane depolarization that inactivates the low-threshold Ca2+ spike, causing a transition from burst to tonic
response mode. The excitatory effects of metabotropic receptor
activation in the LGN appear to be produced through the receptors
linked to phosphoinositide hydrolysis and apparently only through
activation of the corticogeniculate pathway. Two mGluRs, mGluR1 (a
splice variant of mGluR1) and mGluR5, are linked to the
phosphoinositide system. We examined the localization of these
receptors with affinity-purified, anti-peptide, polyclonal antibodies
raised to the C-terminal region of each receptor protein. Under
examination with the light microscope, we found that both types of
receptors are present in the geniculate neuropil and in that of the
overlying thalamic reticular nucleus, including the perigeniculate
nucleus. We also examined the ultrastructural localization of
immunolabel with the electron microscope, using a postembedding
immunogold marker to identify terminals, dendrites, and somata that
contain GABA. Label for the antibody directed against mGluR1 was
primarily localized in the dendrites of relay cells, postsynaptic to
various terminal types. Of these, terminal profiles normally associated
with corticogeniculate inputs predominated, whereas retinal terminal
profiles were scarce. Label for the antibody directed against mGluR5
label was prominent in inhibitory F2-terminal profiles associated with
the retinal input to relay cells. In the perigeniculate nucleus, both
mGluRs were localized to dendrites. The distribution of the two
phosphoinositide-linked mGluRs in the LGN suggests very different
functional roles for the two receptor types. We conclude from these
data that mGluR1 appears to have a dominant role in corticogeniculate
control of response mode through the feedback glutamatergic pathway
from layer VI, whereas mGluR5 is positioned to affect retinogeniculate
activation of relay cells through feed forward glomerular
interactions.
Key words:
thalamus;
vision;
interneurons;
corticogeniculate;
glomerulus;
immunocytochemistry
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