 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
Volume 16, Number 9, Issue of May 1, 1996 pp. 2881-2890
Copyright ©1996 Society for NeuroscienceInhibition of cGMP Breakdown Promotes the Induction of Cerebellar Long-Term Depression
Received Aug. 8, 1995; revised Feb. 1, 1996; accepted Feb. 5, 1996.
Nick A. Hartell Laboratory for Synaptic Function, Frontier Research Program, RIKEN, Wako-shi, Saitama, 351-01, Japan
The effects of the nonspecific cyclic nucleotide inhibitors 1-methyl-3-isobutylxanthine (IBMX) and dipyridamole, and the cGMP-specific phosphodiesterase inhibitor Zaprinast were studied on parallel fiber-Purkinje cell synaptic responses in rat cerebellar slices. Bath application of all three compounds, at concentrations shown to inhibit cGMP breakdown, led to stable and robust long-term depression of PF responses. Injections of dipyridamole directly into the Purkinje cell dendrites were similarly effective as bath applications, confirming a postsynaptic site of action. Inhibitors of both protein kinase G and C and also the metabotropic glutamate receptor antagonist MCPG completely prevented the induction of LTD by dipyridamole and Zaprinast. The extent of phosphodiesterase-induced synaptic depression was dependent on the frequency of parallel fiber stimulation, and this form of LTD both occluded and was occluded by LTD induced by pairing parallel and climbing fiber inputs. The degree of LTD induced by IBMX was dose-dependent, and also required PKC and PKG activity, but was preceded by a large, transient potentiation of parallel fiber responses occurring by a postsynaptic mechanism independent of cGMP. These data not only confirm that cGMP is capable of inducing cerebellar LTD when paired with parallel fiber stimulation but indicate that cGMP is an endogenous intermediate in this form of synaptic plasticity.
Key words: cGMP; phosphodiesterase inhibitor; cerebellum; Purkinje cell; long-term depression; climbing fiber
This article has been cited by other articles:
R. Bouley, N. Pastor-Soler, O. Cohen, M. McLaughlin, S. Breton, and D. Brown
Stimulation of AQP2 membrane insertion in renal epithelial cells in vitro and in vivo by the cGMP phosphodiesterase inhibitor sildenafil citrate (Viagra)
Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1103 - F1112.
[Abstract] [Full Text] [PDF]
R. Feil, J. Hartmann, C. Luo, W. Wolfsgruber, K. Schilling, S. Feil, J. J. Barski, M. Meyer, A. Konnerth, C. I. De Zeeuw, et al.
Impairment of LTD and cerebellar learning by Purkinje cell-specific ablation of cGMP-dependent protein kinase I
J. Cell Biol., October 27, 2003; 163(2): 295 - 302.
[Abstract] [Full Text] [PDF]
M. Shimizu-Albergine, S. D. Rybalkin, I. G. Rybalkina, R. Feil, W. Wolfsgruber, F. Hofmann, and J. A. Beavo
Individual Cerebellar Purkinje Cells Express Different cGMP Phosphodiesterases (PDEs): In Vivo Phosphorylation of cGMP-Specific PDE (PDE5) as an Indicator of cGMP-dependent protein kinase (PKG) Activation
J. Neurosci., July 23, 2003; 23(16): 6452 - 6459.
[Abstract] [Full Text] [PDF]
R. Zhang, Y. Wang, L. Zhang, Z. Zhang, W. Tsang, M. Lu, L. Zhang, and M. Chopp
Sildenafil (Viagra) Induces Neurogenesis and Promotes Functional Recovery After Stroke in Rats
Stroke, November 1, 2002; 33(11): 2675 - 2680.
[Abstract] [Full Text] [PDF]
S. Saksena, R. K. Gill, I. A. Syed, S. Tyagi, W. A. Alrefai, K. Ramaswamy, and P. K. Dudeja
Modulation of Cl-/OH- exchange activity in Caco-2 cells by nitric oxide
Am J Physiol Gastrointest Liver Physiol, September 1, 2002; 283(3): G626 - G633.
[Abstract] [Full Text] [PDF]
V. Lev-Ram, S. T. Wong, D. R. Storm, and R. Y. Tsien
A new form of cerebellar long-term potentiation is postsynaptic and depends on nitric oxide but not cAMP
PNAS, June 11, 2002; 99(12): 8389 - 8393.
[Abstract] [Full Text] [PDF]
S. G. Andreeva, P. Dikkes, P. M. Epstein, and P. A. Rosenberg
Expression of cGMP-Specific Phosphodiesterase 9A mRNA in the Rat Brain
J. Neurosci., November 15, 2001; 21(22): 9068 - 9076.
[Abstract] [Full Text] [PDF]
S. Jacoby, R. E Sims, and N. A Hartell
Nitric oxide is required for the induction and heterosynaptic spread of long-term potentiation in rat cerebellar slices
J. Physiol., September 15, 2001; 535(3): 825 - 839.
[Abstract] [Full Text] [PDF]
M. Ito
Cerebellar Long-Term Depression: Characterization, Signal Transduction, and Functional Roles
Physiol Rev, July 1, 2001; 81(3): 1143 - 1195.
[Abstract] [Full Text] [PDF]
M. Casado, S. Dieudonné, and P. Ascher
Presynaptic N-methyl-D-aspartate receptors at the parallel fiber-Purkinje cell synapse
PNAS, September 29, 2000; (2000) 200354297.
[Abstract] [Full Text]
T Reynolds and N A Hartell
An evaluation of the synapse specificity of long-term depression induced in rat cerebellar slices
J. Physiol., September 15, 2000; 527(3): 563 - 577.
[Abstract] [Full Text] [PDF]
S. S.-H. Wang, L. Khiroug, and G. J. Augustine
Quantification of spread of cerebellar long-term depression with chemical two-photon uncaging of glutamate
PNAS, July 5, 2000; (2000) 130414597.
[Abstract] [Full Text]
J. Kotera, K. Fujishige, and K. Omori
Immunohistochemical Localization of cGMP-binding cGMP-specific Phosphodiesterase (PDE5) in Rat Tissues
J. Histochem. Cytochem., May 1, 2000; 48(5): 685 - 694.
[Abstract] [Full Text]
P. Calabresi, P. Gubellini, D. Centonze, G. Sancesario, M. Morello, M. Giorgi, A. Pisani, and G. Bernardi
A Critical Role of the Nitric Oxide/cGMP Pathway in Corticostriatal Long-Term Depression
J. Neurosci., April 1, 1999; 19(7): 2489 - 2499.
[Abstract] [Full Text] [PDF]
A. Nighorn, K. A. Byrnes, and D. B. Morton
Identification and Characterization of a Novel beta Subunit of Soluble Guanylyl Cyclase That Is Active in the Absence of a Second Subunit and Is Relatively Insensitive to Nitric Oxide
J. Biol. Chem., January 22, 1999; 274(4): 2525 - 2531.
[Abstract] [Full Text] [PDF]
H. Wakatsuki, H. Gomi, M. Kudoh, S. Kimura, K. Takahashi, M. Takeda, and K. Shibuki
Layer-specific NO dependence of long-term potentiation and biased NO release in layer V in the rat auditory cortex
J. Physiol., November 15, 1998; 513(1): 71 - 81.
[Abstract] [Full Text] [PDF]
S. Kimura, S. Uchiyama, H. E. Takahashi, and K. Shibuki
cAMP-Dependent Long-Term Potentiation of Nitric Oxide Release from Cerebellar Parallel Fibers in Rats
J. Neurosci., November 1, 1998; 18(21): 8551 - 8558.
[Abstract] [Full Text] [PDF]
S. E. Farinelli, L. A. Greene, and W. J. Friedman
Neuroprotective Actions of Dipyridamole on Cultured CNS Neurons
J. Neurosci., July 15, 1998; 18(14): 5112 - 5123.
[Abstract] [Full Text] [PDF]
J. Wu, Y. Wang, M. J. Rowan, and R. Anwyl
Evidence for Involvement of the cGMP-Protein Kinase G Signaling System in the Induction of Long-Term Depression, But Not Long-Term Potentiation, in the Dentate Gyrus In Vitro
J. Neurosci., May 15, 1998; 18(10): 3589 - 3596.
[Abstract] [Full Text] [PDF]
J Eilers, H Takechi, E A Finch, G J Augustine, and A Konnerth
Local dendritic Ca2+ signaling induces cerebellar long-term depression.
Learn. Mem., January 1, 1997; 4(1): 159 - 168.
[Abstract] [PDF]
S. S.-H. Wang, L. Khiroug, and G. J. Augustine
Quantification of spread of cerebellar long-term depression with chemical two-photon uncaging of glutamate
PNAS, July 18, 2000; 97(15): 8635 - 8640.
[Abstract] [Full Text] [PDF]
M. Casado, S. Dieudonne, and P. Ascher
Presynaptic N-methyl-D-aspartate receptors at the parallel fiber-Purkinje cell synapse
PNAS, October 10, 2000; 97(21): 11593 - 11597.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|