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Previous Article | Next Article
Volume 16, Number 9, Issue of May 1, 1996 pp. 2934-2944
Copyright ©1996 Society for NeuroscienceGlutamate Responses of Bipolar Cells in a Slice Preparation of the Rat Retina
Received Jan. 16, 1996; accepted Feb. 9, 1996.
Thomas Euler, Horst Schneider, and Heinz Wässle Max-Planck-Institut für Hirnforschung, Deutschordenstrasse 46, D-60528 Frankfurt, Germany
Whole-cell currents from >70 voltage-clamped bipolar cells were recorded in a slice preparation of the rat retina. The recorded cells were identified and classified by intracellular staining with Lucifer yellow. Glutamate, the specific agonists (±)-2-amino-4-phosphonobutyric acid (AP-4) and kainate (KA), and the antagonist 6-cyanoquinoxaline-2,3-dione (CNQX) were applied. The cells could be isolated from presynaptic influences by the co-application of bicuculline, strychnine, and cobalt ions. Responses to AP-4 were elicited only from bipolar cells with axons stratifying in the inner part of the inner plexiform layer (IPL). AP-4 caused an outward current in these cells attributable to the closure of nonspecific cation channels. Responses to kainate representing a direct action of the drug on the recorded cells were observed only in bipolar cells with axons stratifying in the outer part of the IPL. KA caused a CNQX-sensitive inward current in these cells, associated with openings of nonspecific cation channels. The results predict that cone bipolar (CB) cells with axons terminating in the outer IPL are OFF-bipolars, whereas those with axons terminating in the inner IPL are ON-bipolars. Most of the cells expressed GABA-gated Cl
conductances. In rod bipolar and in some CB cells, only part of the GABA-induced currents could be blocked by the application of bicuculline, suggesting the presence of GABAC receptors in addition to GABAA receptors.
Key words: retina; slice; whole-cell currents; cone bipolar cells; rod bipolar cells; ON-cells; OFF-cells; AP-4 receptor; glutamate receptor; GABAC receptor
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