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Volume 16, Number 9,
Issue of May 1, 1996
pp. 2956-2971
Copyright ©1996 Society for Neuroscience
Regulation of  7 Nicotinic Acetylcholine Receptors
in the Developing Rat Somatosensory Cortex by Thalamocortical
Afferents
Received Oct. 10, 1995; revised Jan. 31, 1996; accepted Feb. 5, 1996.
Ron S. Broide1,
Richard T. Robertson2, and
Frances M. Leslie1
Departments of 1 Pharmacology and 2 Anatomy
and Neurobiology, College of Medicine, University of California,
Irvine, California 92717
Distributions of  7 nicotinic acetylcholine
receptor (nAChR) mRNA and
[125I]-bungarotoxin (-BTX) binding sites
in the developing rat somatosensory cortex were characterized in
relation to acetylcholinesterase (AChE) histochemical staining of
thalamocortical terminals to investigate the role of this receptor in
cortical development. Using quantitative in situ
hybridization and receptor autoradiography, elevated levels of mRNA and
binding-site expression were first detected at postnatal day 1 (P1) in
deep and superficial layers, just beneath the AChE-stained
thalamocortical terminals. Onset of expression occurred ~1 d after
ingrowth of AChE-stained thalamocortical afferents. By P5, mRNA and
binding-site expression exhibited a disjunctive, barrel-like pattern in
layer IV and, more clearly, in layer VI. The mRNA and binding-site
expressions peaked at ~1 week postnatal and then declined to adult
levels. Unilateral electrolytic or cytochemical lesions placed in the
thalamic ventrobasal complex at P0 (just as thalamocortical afferents
are innervating the cortex) and at P6 (when the somatotopic map is well
established) resulted in a marked reduction of
7 nAChR mRNA and
[125I]-BTX binding-site levels in layers IV
and VI, indicating their regulation by thalamocortical afferents. With
P6 lesions, this reduction was observed as early as 6 hr postlesion.
These results suggest that 7 nAChRs are
localized primarily on cortical cells in rat somatosensory cortex and
provide further evidence for thalamocortical influence on cortical
ontogeny. These data also suggest a role for cholinergic systems during
a critical period of cortical synaptogenesis.
Key words:
nicotinic;
bungarotoxin;
cholinergic;
thalamocortical;
barrels;
cortical development
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