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Volume 16, Number 9, Issue of May 1, 1996 pp. 2983-2994
Copyright ©1996 Society for NeuroscienceThe Dynamics of Dendritic Structure in Developing Hippocampal Slices
Received Nov. 27, 1995; revised Feb. 8, 1996; accepted Feb. 14, 1996.
Michael E. Dailey and Stephen J Smith Department of Molecular and Cellular Physiology, Beckman Center, Stanford University Medical School, Stanford, California 94305-5426
Time-lapse fluorescence confocal microscopy was used to directly visualize the formation and dynamics of postsynaptic target structures (i.e., dendritic branches and spines) on pyramidal neurons within developing hippocampal tissue slices. Within a 2 week period of time, pyramidal neurons in cultured slices derived from early postnatal rat (postnatal days 2-7) developed complex dendritic arbors bearing numerous postsynaptic spines. At early stages (1-2 d in vitro), many fine filopodial protrusions on dendrite shafts rapidly extended (maximum rate ~2.5 µm/min) and retracted (median filopodial lifetime, 10 min), but some filopodia transformed into growth cones and nascent dendrite branches. As dendritic arbors matured, the population of fleeting lateral filopodia was replaced by spine-like structures having a low rate of turnover. This developmental progression involved a transitional stage in which dendrites were dominated by persistent (up to 22 hr) but dynamic spiny protrusions (i.e., protospines) that showed substantial changes in length and shape on a timescale of minutes. These observations reveal a highly dynamic state of postsynaptic target structures that may actively contribute to the formation and plasticity of synaptic connections during CNS development.
Key words: dendrite; dendritic spine; development; pyramidal cell; hippocampal slice; DiI; confocal imaging; time-lapse
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