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Volume 17, Number 1,
Issue of January 1, 1997
pp. 125-139
Copyright ©1997 Society for Neuroscience
Developmental Expression of Platelet-Derived Growth
Factor  -Receptor in Neurons and Glial Cells of the Mouse CNS
Received July 29, 1996; revised Oct. 9, 1996; accepted Oct. 21, 1996.
Brahim Nait Oumesmar,
Lionel Vignais, and
Anne Baron-Van Evercooren
Institut National de la Santé et de la Recherche
Médicale U134, Laboratoire de Neurobiologie Cellulaire,
Moleculaire et Clinique and CJF Laboratoire de la Pathologie de la
Myéline, 75651 Paris Cedex 13, France
The synthesis of platelet-derived growth factor- receptor
(PDGF-R) is commonly attributed to oligodendrocyte progenitors during late embryonic and postnatal development. However, we recently demonstrated that mature neurons could also synthesize PDGF-R, emphasizing a larger role for this receptor than previously described. In the present study, to analyze the pattern of PDGF-R expression during postnatal development of the mouse CNS, we used in
situ hybridization and immunohistochemistry on brain and spinal
cord tissue sections. We found that, in addition to immature cells of
the oligodendrocyte lineage, neurons of various CNS regions express
PDGF-R transcripts and protein as early as postnatal day 1 (P1).
Whereas neuronal expression was maintained at all ages, the
oligodendroglial expression strongly decreased after P21. In the adult,
PDGF-R was detected in very few oligodendrocyte progenitors
scattered in the cerebral cortex or in white matter tracts, thus
suggesting the presence of PDGF-R on O-2Aadult
progenitors. In the mature CNS, PDGF-R transcripts and protein were
mainly localized in neurons of numerous structures, such as the
olfactory bulb, cerebral cortex, hippocampus, and brainstem nuclei and
in motor neurons of the ventral horn of the spinal cord. The
differential expression of PDGF-R in oligodendroglia and neurons
argues in favor of several roles of PDGF during development.
Key words:
platelet-derived growth factor;
PDGF -receptor;
oligodendrocyte lineage;
neurons;
CNS development;
in situ
hybridization;
immunohistochemistry
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