 |
 Previous Article | Next Article 
Volume 17, Number 10,
Issue of May 15, 1997
pp. 3580-3587
Copyright ©1997 Society for Neuroscience
Bitter Taste Transduction of Denatonium in the Mudpuppy
Necturus maculosus
Received Jan. 22, 1997; revised March 3, 1997; accepted March 5, 1997.
Tatsuya Ogura1,
Alan Mackay-Sim1, 2, and
Sue C. Kinnamon1
1 Department of Anatomy and Neurobiology, Colorado
State University, Fort Collins, Colorado 80523, and Rocky Mountain
Taste and Smell Center, University of Colorado Health Sciences Center,
Denver, Colorado 80262, and 2 School of Biomolecular and
Biomedical Science, Griffith University, Nathan, QLD 4111 Australia
Bitter substances are a structurally diverse group of compounds
that appear to act via several transduction mechanisms. The bitter-tasting denatonium ion has been proposed to act via two different G-protein-regulated pathways, one involving inositol 1,4,5-trisphosphate and raised intracellular calcium levels, the other
involving phosphodiesterase and membrane depolarization via a cyclic
nucleotide-suppressible cation channel. The aim of the present study
was to examine these transduction mechanisms in taste cells of the
mudpuppy Necturus maculosus by calcium-imaging and
whole-cell recording. Denatonium benzoate increased intracellular calcium levels and induced an outward current independently of extracellular calcium. The denatonium-induced increase in intracellular calcium was inhibited by U73122, an inhibitor of phospholipase C, and
by thapsigargin, an inhibitor of calcium transport into intracellular
stores. The denatonium-induced outward current was blocked by
GDP- -S, a blocker of G-protein activation. Neither resting nor
denatonium-induced intracellular calcium levels were affected by
inhibition of phosphodiesterase (with IBMX) or adenylate cyclase
(with SQ22536) or by raising intracellular cyclic nucleotides directly
(with cell permeant analogs). Our results support the hypothesis that
denatonium is transduced via a G-protein cascade involving
phospholipase C, inositol 1,4,5-trisphosphate, and raised intracellular
calcium levels. Our results do not support the hypothesis that
denatonium is transduced via phosphodiesterase and cAMP.
Key words:
bitter taste transduction;
mudpuppy;
taste receptor
cells;
fura-2;
calcium imaging;
whole-cell recording
This article has been cited by other articles:
|
|
|
|
 
K. Hacker and K. F. Medler
Mitochondrial Calcium Buffering Contributes to the Maintenance of Basal Calcium Levels in Mouse Taste Cells
J Neurophysiol,
October 1, 2008;
100(4):
2177 - 2191.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Lin, T. Ogura, R. F. Margolskee, T. E. Finger, and D. Restrepo
TRPM5-Expressing Solitary Chemosensory Cells Respond to Odorous Irritants
J Neurophysiol,
March 1, 2008;
99(3):
1451 - 1460.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Zhang, Z. Zhao, R. Margolskee, and E. Liman
The Transduction Channel TRPM5 Is Gated by Intracellular Calcium in Taste Cells
J. Neurosci.,
May 23, 2007;
27(21):
5777 - 5786.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ogura and W. Lin
Acetylcholine and Acetylcholine Receptors in Taste Receptor Cells
Chem Senses,
January 1, 2005;
30(suppl_1):
i41 - i41.
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Liu and E. R. Liman
Intracellular Ca2+ and the phospholipid PIP2 regulate the taste transduction ion channel TRPM5
PNAS,
December 9, 2003;
100(25):
15160 - 15165.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ogura
Acetylcholine Increases Intracellular Ca2+ in Taste Cells Via Activation of Muscarinic Receptors
J Neurophysiol,
June 1, 2002;
87(6):
2643 - 2649.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ogura, R. F. Margolskee, and S. C. Kinnamon
Taste Receptor Cell Responses to the Bitter Stimulus Denatonium Involve Ca2+ Influx Via Store-Operated Channels
J Neurophysiol,
June 1, 2002;
87(6):
3152 - 3155.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Cubero-Castillo and A.C. Noble
Effect of Compound Sequence on Bitterness Enhancement
Chem Senses,
May 1, 2001;
26(4):
419 - 424.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Miyoshi, K. Abe, and Y. Emori
IP3 receptor type 3 and PLC{beta}2 are co-expressed with taste receptors T1R and T2R in rat taste bud cells
Chem Senses,
April 1, 2001;
26(3):
259 - 265.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Yan, G. Sunavala, S. Rosenzweig, M. Dasso, J. G. Brand, and A. I. Spielman
Bitter taste transduced by PLC-{beta}2-dependent rise in IP3 and {alpha}-gustducin-dependent fall in cyclic nucleotides
Am J Physiol Cell Physiol,
April 1, 2001;
280(4):
C742 - C751.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Caicedo and S. D. Roper
Taste Receptor Cells That Discriminate Between Bitter Stimuli
Science,
February 23, 2001;
291(5508):
1557 - 1560.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Caicedo, M. S. Jafri, and S. D. Roper
In Situ Ca2+ Imaging Reveals Neurotransmitter Receptors for Glutamate in Taste Receptor Cells
J. Neurosci.,
November 1, 2000;
20(21):
7978 - 7985.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kusakabe, A. Yasuoka, M. Asano-Miyoshi, K. Iwabuchi, I. Matsumoto, S. Arai, Y. Emori, and K. Abe
Comprehensive Study on G protein {alpha}-Subunits in Taste Bud Cells, with Special Reference to the Occurrence of G{alpha}i2 as a Major G{alpha} Species
Chem Senses,
October 1, 2000;
25(5):
525 - 531.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ogura and S. C. Kinnamon
IP3-Independent Release of Ca2+ From Intracellular Stores: A Novel Mechanism for Transduction of Bitter Stimuli
J Neurophysiol,
November 1, 1999;
82(5):
2657 - 2666.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Rosenzweig, W. Yan, M. Dasso, and A. I. Spielman
Possible Novel Mechanism for Bitter Taste Mediated Through cGMP
J Neurophysiol,
April 1, 1999;
81(4):
1661 - 1665.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Kroner, K. Eybrechts, and J.-W. N. Akkerman
Dual Regulation of Platelet Protein Kinase B
J. Biol. Chem.,
September 1, 2000;
275(36):
27790 - 27798.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
