QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (75) Citing Articles via Google Scholar Google Scholar Articles by Rostworowski, M. Articles by Yong, V. W. Search for Related Content PubMed PubMed Citation Articles by Rostworowski, M. Articles by Yong, V. W.

 Previous Article  |  Next Article 

Volume 17, Number 10, Issue of May 15, 1997 pp. 3664-3674
Copyright ©1997 Society for Neuroscience

Astrogliosis in the Neonatal and Adult Murine Brain Post-Trauma: Elevation of Inflammatory Cytokines and the Lack of Requirement for Endogenous Interferon-

Received Jan. 6, 1997; revised Feb. 18, 1997; accepted Feb. 24, 1997.

Maria Rostworowski¹, Vijayabalan Balasingam¹, Sophie Chabot^{1, 2}, Trevor Owens¹, and Voon Wee Yong^{1, 2}

¹ Montreal Neurological Institute, McGill University, Montreal, Quebec H3A 2B4, Canada, and ² Neuroscience Research Group, University of Calgary, Calgary NW, Alberta T2N 4N1, Canada

The relevance of astrogliosis remains controversial, especially with respect to the beneficial or detrimental influence of reactive astrocytes on CNS recovery. This dichotomy can be resolved if the mediators of astrogliosis are identified. We have measured the levels of transcripts encoding inflammatory cytokines in injury systems in which the presence or absence of astrogliosis could be produced selectively. A stab injury to the adult mouse brain using a piece of nitrocellulose (NC) membrane elicited a prompt and marked increase in levels of transcripts for interleukin (IL)-1, IL-1, and tumor necrosis factor (TNF)-, which are considered to be microglia/macrophage cytokines. The elevations preceded, or occurred concomitantly with, the rise in glial fibrillary acidic protein mRNA, an early manifestation of astrogliosis. In neonatal mice, IL-1 and TNF- mRNA were elevated to a greater extent by an NC-implant injury, which produced astrogliosis, than after an NC-stab, with minimal astrogliosis. We determined whether endogenous interferon (IFN)- could be responsible for the observed increases in IL-1 and TNF-, because IFN- is a potent microglia/macrophage activator, and because its exogenous administration to rodents enhanced astrogliosis after adult or neonatal insults. A lack of requirement for endogenous IFN- was demonstrated by three lines of evidence. First, no increase in IFN- transcripts could be found at injury. Second, the administration of a neutralizing antibody to IFN- did not attenuate astrogliosis. Third, in IFN- knockout adult mice, astrogliosis and increases in levels of IL-1 and TNF- were induced rapidly by injury. The marked elevation of inflammatory cytokines is discussed in the context of astrogliosis and general CNS recovery.

Key words: CNS trauma; cytokines; gliosis; interleukin-1; interferon-; microglia; reactive astrocytes; TNF-

This article has been cited by other articles:

				A. A. Babcock, H. Toft-Hansen, and T. Owens Signaling through MyD88 Regulates Leukocyte Recruitment after Brain Injury J. Immunol., November 1, 2008; 181(9): 6481 - 6490. [Abstract] [Full Text] [PDF]

				F. E. Perrin, S. Lacroix, M. Aviles-Trigueros, and S. David Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1{alpha} and interleukin-1{beta} in Wallerian degeneration Brain, April 1, 2005; 128(4): 854 - 866. [Abstract] [Full Text] [PDF]

				M. Morita, C. Higuchi, T. Moto, N. Kozuka, J. Susuki, R. Itofusa, J. Yamashita, and Y. Kudo Dual Regulation of Calcium Oscillation in Astrocytes by Growth Factors and Pro-Inflammatory Cytokines via the Mitogen-Activated Protein Kinase Cascade J. Neurosci., November 26, 2003; 23(34): 10944 - 10952. [Abstract] [Full Text] [PDF]

				J. E. A. Wells, T. K. Rice, R. K. Nuttall, D. R. Edwards, H. Zekki, S. Rivest, and V. W. Yong An Adverse Role for Matrix Metalloproteinase 12 after Spinal Cord Injury in Mice J. Neurosci., November 5, 2003; 23(31): 10107 - 10115. [Abstract] [Full Text] [PDF]

				G. R. John, S. C. Lee, and C. F. Brosnan Cytokines: Powerful Regulators of Glial Cell Activation Neuroscientist, February 1, 2003; 9(1): 10 - 22. [Abstract] [PDF]

				E. A. Eugenín, D. Eckardt, M. Theis, K. Willecke, M. V. L. Bennett, and J. C. Sáez Microglia at brain stab wounds express connexin 43 and in vitro form functional gap junctions after treatment with interferon-gamma and tumor necrosis factor-alpha PNAS, March 16, 2001; (2001) 51634298. [Abstract] [Full Text]

				L. M. Herx, S. Rivest, and V. W. Yong Central Nervous System-Initiated Inflammation and Neurotrophism in Trauma: IL-1{beta} Is Required for the Production of Ciliary Neurotrophic Factor J. Immunol., August 15, 2000; 165(4): 2232 - 2239. [Abstract] [Full Text] [PDF]

				M. B. Jensen, I. V. Hegelund, N. D. Lomholt, B. Finsen, and T. Owens IFNgamma Enhances Microglial Reactions to Hippocampal Axonal Degeneration J. Neurosci., May 15, 2000; 20(10): 3612 - 3621. [Abstract] [Full Text] [PDF]

				E. A. Eugenin, D. Eckardt, M. Theis, K. Willecke, M. V. L. Bennett, and J. C. Saez Microglia at brain stab wounds express connexin 43 and in vitro form functional gap junctions after treatment with interferon-gamma and tumor necrosis factor-alpha PNAS, March 27, 2001; 98(7): 4190 - 4195. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help