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Volume 17, Number 12,
Issue of June 15, 1997
pp. 4700-4710
Copyright ©1997 Society for Neuroscience
The shaking-B2 Mutation Disrupts
Electrical Synapses in a Flight Circuit in Adult
Drosophila
Received Jan. 30, 1997; revised March 27, 1997; accepted March 28, 1997.
James R. Trimarchi and
R. K. Murphey
Department of Biology, Molecular and Cellular Biology Program,
Morrill Science Center, University of Massachusetts, Amherst,
Massachusetts 01003
The shaking-B2 mutation was used to
analyze synapses between haltere afferents and a flight motoneuron in
adult Drosophila. We show that the electrical synapses
among many neurons in the flight circuit are disrupted in
shaking-B2 flies, suggesting that
shaking-B expression is required for electrical synapses
throughout the nervous system. In wild-type flies haltere afferents are
dye-coupled to the first basalar motoneuron, and stimulation of these
afferents evokes electromyograms from the first basalar muscle with
short latencies. In shaking-B2 flies dye
coupling between haltere afferents and the motoneuron is abolished, and
afferent stimulation evokes electromyograms at abnormally long
latencies. Intracellular recordings from the motoneuron confirm that
the site of the defect in shaking-B2 flies
is at the synapses between haltere afferents and the flight motoneuron.
The nicotinic cholinergic antagonist mecamylamine blocks the
haltere-to-flight motoneuron synapses in
shaking-B2 flies but does not block those
synapses in wild-type flies. Together, these results show that the
haltere-to-flight motoneuron synapses comprise an electrical component
that requires shaking-B and a chemical component that is
likely to be cholinergic.
Key words:
gap junctions, Drosophila, shaking-B,
Passover, synapse, flight
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