QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (67) Citing Articles via Google Scholar Google Scholar Articles by Hibino, H. Articles by Kurachi, Y. Search for Related Content PubMed PubMed Citation Articles by Hibino, H. Articles by Kurachi, Y.

 Previous Article  |  Next Article 

Volume 17, Number 12, Issue of June 15, 1997 pp. 4711-4721
Copyright ©1997 Society for Neuroscience

An ATP-Dependent Inwardly Rectifying Potassium Channel, K_AB-2 (Kir4.1), in Cochlear Stria Vascularis of Inner Ear: Its Specific Subcellular Localization and Correlation with the Formation of Endocochlear Potential

Received Jan. 21, 1997; revised March 17, 1997; accepted March 31, 1997.

Hiroshi Hibino^{1, 2}, Yoshiyuki Horio¹, Atsushi Inanobe⁴, Katsumi Doi², Minoru Ito⁴, Mitsuhiko Yamada¹, Takahiro Gotow³, Yasuo Uchiyama³, Masaru Kawamura⁵, Takeshi Kubo², and Yoshihisa Kurachi^{1, 4}

Departments of ¹ Pharmacology II, ² Otolaryngology, and ³ Anatomy I, Faculty of Medicine, Osaka University, Osaka 565, Japan, ⁴ Department of Cell Biology and Signaling, Yamagata University School of Medicine, Yamagata 990-23, Japan, and ⁵ Department of Biology, University of Occupational and Environmental Health, Kitakyushu 807, Japan

Cochlear endolymph has a highly positive potential of approximately +80 mV. This so-called endocochlear potential (EP) is essential for hearing. Although pivotal roles of K⁺ channels in the formation of EP have been suggested, the types and distribution of K⁺ channels in cochlea have not been characterized. Because EP was depressed by vascular perfusion of Ba²⁺, an inhibitor of inwardly rectifying K⁺ (Kir) channels, but not by either 4-aminopyridine or tetraethylammonium, we examined the expression of Kir channel subunits in cochlear stria vascularis, the tissue that is supposed to play the central role in the generation of positive EP. Of 11 members of the Kir channel family examined with reverse transcription-PCR, we could detect only expression of K_AB-2 (Kir4.1) mRNA in stria vascularis. K_AB-2 immunoreactivity was specifically localized at the basolateral membrane of marginal cells but not in either basal or intermediate cells. Developmental expression of K_AB-2 in marginal cells paralleled formation of EP. Furthermore, deaf mutant mice (viable dominant spotting; W^V/W^V) expressed no K_AB-2 in their marginal cells. These results suggest that K_AB-2 in marginal cells may be critically involved in the generation of positive EP.

Key words: inwardly rectifying potassium channel; endocochlear potential; stria vascularis; vestibule; development; W^V/W^V mice

This article has been cited by other articles:

				F. Nin, H. Hibino, K. Doi, T. Suzuki, Y. Hisa, and Y. Kurachi The endocochlear potential depends on two K+ diffusion potentials and an electrical barrier in the stria vascularis of the inner ear PNAS, February 5, 2008; 105(5): 1751 - 1756. [Abstract] [Full Text] [PDF]

				F. Lang, V. Vallon, M. Knipper, and P. Wangemann Functional significance of channels and transporters expressed in the inner ear and kidney Am J Physiol Cell Physiol, October 1, 2007; 293(4): C1187 - C1208. [Abstract] [Full Text] [PDF]

				J. Gao, S. F. Maison, X. Wu, K. Hirose, S. M. Jones, I. Bayazitov, Y. Tian, G. Mittleman, D. B. Matthews, S. S. Zakharenko, et al. Orphan Glutamate Receptor {delta}1 Subunit Required for High-Frequency Hearing Mol. Cell. Biol., June 15, 2007; 27(12): 4500 - 4512. [Abstract] [Full Text] [PDF]

				M. Kono, I. A. Belyantseva, A. Skoura, G. I. Frolenkov, M. F. Starost, J. L. Dreier, D. Lidington, S.-S. Bolz, T. B. Friedman, T. Hla, et al. Deafness and Stria Vascularis Defects in S1P2 Receptor-null Mice J. Biol. Chem., April 6, 2007; 282(14): 10690 - 10696. [Abstract] [Full Text] [PDF]

				T. Shibata, H. Hibino, K. Doi, T. Suzuki, Y. Hisa, and Y. Kurachi Gastric type H+,K+-ATPase in the cochlear lateral wall is critically involved in formation of the endocochlear potential Am J Physiol Cell Physiol, November 1, 2006; 291(5): C1038 - C1048. [Abstract] [Full Text] [PDF]

				H. Hibino and Y. Kurachi Molecular and physiological bases of the k+ circulation in the Mammalian inner ear. Physiology, October 1, 2006; 21: 336 - 345. [Abstract] [Full Text] [PDF]

				L. Nie, M. A. Gratton, K. J. Mu, J. N. Dinglasan, W. Feng, and E. N. Yamoah Expression and Functional Phenotype of Mouse ERG K+ Channels in the Inner Ear: Potential Role in K+ Regulation in the Inner Ear J. Neurosci., September 21, 2005; 25(38): 8671 - 8679. [Abstract] [Full Text] [PDF]

				L. Nie, W. Feng, R. Diaz, M. A. Gratton, K. J. Doyle, and E. N. Yamoah Functional Consequences of Polyamine Synthesis Inhibition by L-{alpha}-Difluoromethylornithine (DFMO): CELLULAR MECHANISMS FOR DFMO-MEDIATED OTOTOXICITY J. Biol. Chem., April 15, 2005; 280(15): 15097 - 15102. [Abstract] [Full Text] [PDF]

				H. Hibino, A. Fujita, K. Iwai, M. Yamada, and Y. Kurachi Differential Assembly of Inwardly Rectifying K+ Channel Subunits, Kir4.1 and Kir5.1, in Brain Astrocytes J. Biol. Chem., October 15, 2004; 279(42): 44065 - 44073. [Abstract] [Full Text] [PDF]

				X. Du, H. Zhang, C. Lopes, T. Mirshahi, T. Rohacs, and D. E. Logothetis Characteristic Interactions with Phosphatidylinositol 4,5-Bisphosphate Determine Regulation of Kir Channels by Diverse Modulators J. Biol. Chem., September 3, 2004; 279(36): 37271 - 37281. [Abstract] [Full Text] [PDF]

				L. Ruttiger, M. Sausbier, U. Zimmermann, H. Winter, C. Braig, J. Engel, M. Knirsch, C. Arntz, P. Langer, B. Hirt, et al. Deletion of the Ca2+-activated potassium (BK) {alpha}-subunit but not the BK{beta}1-subunit leads to progressive hearing loss PNAS, August 31, 2004; 101(35): 12922 - 12927. [Abstract] [Full Text] [PDF]

				M A Moreno-Pelayo, S Modamio-Hoybjor, A Mencia, I del Castillo, S Chardenoux, M Fernandez-Burriel, M Lathrop, C Petit, and F Moreno DFNA49, a novel locus for autosomal dominant non-syndromic hearing loss, maps proximal to DFNA7/DFNM1 region on chromosome 1q21-q23 J. Med. Genet., November 1, 2003; 40(11): 832 - 836. [Full Text] [PDF]

				D. C. Marcus, T. Wu, P. Wangemann, and P. Kofuji KCNJ10 (Kir4.1) potassium channel knockout abolishes endocochlear potential Am J Physiol Cell Physiol, February 1, 2002; 282(2): C403 - C407. [Abstract] [Full Text] [PDF]

				K. Higashi, A. Fujita, A. Inanobe, M. Tanemoto, K. Doi, T. Kubo, and Y. Kurachi An inwardly rectifying K+ channel, Kir4.1, expressed in astrocytes surrounds synapses and blood vessels in brain Am J Physiol Cell Physiol, September 1, 2001; 281(3): C922 - C931. [Abstract] [Full Text] [PDF]

				P. Kofuji, P. Ceelen, K. R. Zahs, L. W. Surbeck, H. A. Lester, and E. A. Newman Genetic Inactivation of an Inwardly Rectifying Potassium Channel (Kir4.1 Subunit) in Mice: Phenotypic Impact in Retina J. Neurosci., August 1, 2000; 20(15): 5733 - 5740. [Abstract] [Full Text] [PDF]

				P. F. Chinnery, C. Elliott, G. R. Green, A. Rees, A. Coulthard, D. M. Turnbull, and T. D. Griffiths The spectrum of hearing loss due to mitochondrial DNA defects Brain, January 1, 2000; 123(1): 82 - 92. [Abstract] [Full Text] [PDF]

				M. R. ABRAHAM, A. JAHANGIR, A. E. ALEKSEEV, and A. TERZIC Channelopathies of inwardly rectifying potassium channels FASEB J, November 1, 1999; 13(14): 1901 - 1910. [Abstract] [Full Text]

				H. Hibino, Y. Horio, A. Fujita, A. Inanobe, K. Doi, T. Gotow, Y. Uchiyama, T. Kubo, and Y. Kurachi Expression of an inwardly rectifying K+ channel, Kir4.1, in satellite cells of rat cochlear ganglia Am J Physiol Cell Physiol, October 1, 1999; 277(4): C638 - C644. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help