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Volume 17, Number 16,
Issue of August 15, 1997
pp. 6075-6085
Copyright ©1997 Society for Neuroscience
GABAergic and glycinergic IPSCs in Ganglion Cells of Rat
Retinal Slices
Received Feb. 26, 1997; revised May 27, 1997; accepted June 2, 1997.
Dario A. Protti1,
Hersch M. Gerschenfeld2, and
Isabel Llano1
1 Arbeitsgruppe Zelluläre Neurobiologie,
Max-Planck-Institut für biophysikalische Chemie, 37070 Göttingen, Germany, and 2 Laboratoire de
Neurobiologie, Ecole Normale Supérieure, 75005 Paris, France
GABAergic and glycinergic IPSCs were studied in identified retinal
ganglion cells (RGCs) of light-adapted rat retinal slices, using
whole-cell recording techniques. GABAergic IPSCs were blocked specifically by SR95531 (3 µM) and bicuculline (3 µM) and glycinergic IPSCs by strychnine (0.3 µM). From 37 RGCs studied, 25 showed exclusively
GABAergic IPSCs, 6 presented only glycinergic IPSCs, and 6 showed both.
This distribution may result from differences in amacrine cells input
rather than from receptor heterogeneity, because both GABA and glycine
elicited Cl -selective currents in all RGCs tested.
TTX markedly reduced GABAergic IPSCs frequency, whereas glycinergic
IPSCs were unaffected. Ca2+-free media, with or
without high Mg2+, blocked TTX-resistant GABAergic
and glycinergic IPSCs. These results suggest that GABAergic IPSCs in
RGCs can be elicited either by Na+-dependent action
potentials or by local Ca2+ influx in medium or
large dendritic field GABAergic amacrine cells, whereas glycinergic
IPSCs are generated by action potential-independent Ca2+ influx in narrow field glycinergic amacrine
cells. Both types of IPSCs had fast rise times and biexponential
decays, but glycinergic IPSC decay was significantly slower than that
of GABAergic IPSCs. An elementary conductance of 54 pS for the
glycine-gated channels was estimated from single-channel events,
clearly detected in the falling phase of glycinergic IPSCs, and from
responses to exogenous glycine.
Key words:
synaptic currents;
GABA;
glycine;
retina;
patch-clamp;
neurotransmitter receptors
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