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Volume 17, Number 16,
Issue of August 15, 1997
pp. 6152-6164
Copyright ©1997 Society for Neuroscience
N-Type Calcium Channels in the Developing Rat Hippocampus:
Subunit, Complex, and Regional Expression
Received March 17, 1997; revised May 18, 1997; accepted May 23, 1997.
Owen T. Jones1, 2,
Geula
M. Bernstein1, 2,
Elizabeth J. Jones1,
Denis G. M. Jugloff1, 2,
Marcus Law1,
Wei Wong1, 2, and
Linda R. Mills1, 3
1 Playfair Neuroscience Unit, Toronto Hospital Research
Institute, Toronto Hospital Western Division, Toronto, Ontario, Canada
M5T 2S8, and Departments of 2 Pharmacology and
3 Physiology, University of Toronto, Toronto, Ontario,
Canada M5S 1A8
The expression of multiple classes of voltage-dependent calcium
channels (VDCCs) allows neurons to tailor calcium signaling to
functionally discrete cellular regions. In the developing hippocampus a
central issue is whether the expression of VDCC subtypes plays a role
in key phases such as migration and synaptogenesis. Using radioligand
binding and immunoblotting, we show that some N-type VDCCs exist before
birth, consistent with a role in migration; however, most N-VDCC
subunit expression is postnatal, coinciding with synaptogenesis.
Immunoprecipitation studies indicate that the increased expression of
N-VDCCs in early development occurs without subunit switching because
there is no change in the fraction of  3 subunits in the
N-VDCC  1B-3 heteromers. Fluorescence
imaging of cell surface N-VDCCs during this period reveals that N-VDCCs are expressed on somata before dendrites and that this expression is
asynchronous between different subfields of the hippocampus (CA3-CA4
before CA1-CA2 and dentate gyrus). Our data argue that N-VDCC
expression is an important cue in the genesis of synaptic transmission
in discrete hippocampal subfields.
Key words:
rat;
development;
hippocampus;
pyramidal neurons;
voltage-dependent calcium channels;
subunits;
dendrites;
 -conotoxin
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