QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (41) Citing Articles via Google Scholar Google Scholar Articles by Reale, V. Articles by Evans, P. D. Search for Related Content PubMed PubMed Citation Articles by Reale, V. Articles by Evans, P. D. Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene Compound via MeSH Substance via MeSH Hazardous Substances DB CALCIUM COMPOUNDS CALCIUM, ELEMENTAL

 Previous Article  |  Next Article 

Volume 17, Number 17, Issue of September 1, 1997 pp. 6545-6553
Copyright ©1997 Society for Neuroscience

Agonist-Specific Coupling of a Cloned Drosophila melanogaster D1-Like Dopamine Receptor to Multiple Second Messenger Pathways by Synthetic Agonists

Received May 6, 1997; revised June 10, 1997; accepted June 12, 1997.

Vincenzina Reale¹, Frances Hannan¹, Linda M. Hall², and Peter D. Evans¹

¹ The Babraham Institute Laboratory of Molecular Signaling, Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, United Kingdom, and ² Department of Biochemical Pharmacology, State University of New York at Buffalo, Buffalo, New York 14260-1200

The mechanism of coupling of a cloned Drosophila D1-like dopamine receptor, DopR99B, to multiple second messenger systems when expressed in Xenopus oocytes is described. The receptor is coupled directly to the generation of a rapid, transient intracellular Ca²⁺ signal, monitored as changes in inward current mediated by the oocyte endogenous Ca²⁺-activated chloride channel, by a pertussis toxin-insensitive G-protein-coupled pathway. The more prolonged receptor-mediated changes in adenylyl cyclase activity are generated by an independent G-protein-coupled pathway that is pertussis toxin-sensitive but calcium-independent, and G-subunits appear to be involved in the transduction of this response. This is the first evidence for the direct coupling of a cloned D1-like dopamine receptor both to the activation of adenylyl cyclase and to the initiation of an intracellular Ca²⁺ signal. The pharmacological profile of both second messenger effects is identical for a range of naturally occurring catecholamine ligands (dopamine > norepinephrine > epinephrine) and for the blockade of dopamine responses by a range of synthetic antagonists. However, the pharmacological profiles of the two second messenger responses differ for a range of synthetic agonists. Thus, the receptor exhibits agonist-specific coupling to second messenger systems for synthetic agonists. This feature could provide a useful tool in the genetic analysis of the roles of the multiple second messenger pathways activated by this receptor, given the likely involvement of dopamine in the processes of learning and memory in the insect nervous system.

Key words: cloned dopamine receptor; Drosophila melanogaster; Xenopus oocyte expression; calcium; adenylyl cyclase; G-protein coupling

This article has been cited by other articles:

				N. Makita, J. Sato, K. Manaka, Y. Shoji, A. Oishi, M. Hashimoto, T. Fujita, and T. Iiri An acquired hypocalciuric hypercalcemia autoantibody induces allosteric transition among active human Ca-sensing receptor conformations PNAS, March 27, 2007; 104(13): 5443 - 5448. [Abstract] [Full Text] [PDF]

				J. W. Truman Steroid hormone secretion in insects comes of age PNAS, June 13, 2006; 103(24): 8909 - 8910. [Full Text] [PDF]

				D. P. Srivastava, E. J. Yu, K. Kennedy, H. Chatwin, V. Reale, M. Hamon, T. Smith, and P. D. Evans Rapid, Nongenomic Responses to Ecdysteroids and Catecholamines Mediated by a Novel Drosophila G-Protein-Coupled Receptor J. Neurosci., June 29, 2005; 25(26): 6145 - 6155. [Abstract] [Full Text] [PDF]

				L. Liu, I. Markus, R. J. Vandenberg, B. A. Neilan, M. Murray, and E. Burcher Molecular identification and characterization of three isoforms of tachykinin NK1-like receptors in the cane toad Bufo marinus Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2004; 287(3): R575 - R585. [Abstract] [Full Text] [PDF]

				E. C. Johnson, S. F. Garczynski, D. Park, J. W. Crim, D. R. Nassel, and P. H. Taghert Identification and characterization of a G protein-coupled receptor for the neuropeptide proctolin in Drosophilamelanogaster PNAS, May 13, 2003; 100(10): 6198 - 6203. [Abstract] [Full Text] [PDF]

				U. Gether Uncovering Molecular Mechanisms Involved in Activation of G Protein-Coupled Receptors Endocr. Rev., February 1, 2000; 21(1): 90 - 113. [Abstract] [Full Text]

				A Weisel-Eichler, G Haspel, and F Libersat Venom of a parasitoid wasp induces prolonged grooming in the cockroach J. Exp. Biol., January 4, 1999; 202(8): 957 - 964. [Abstract] [PDF]

				B. L. Wiens, C. S. Nelson, and K. A. Neve Contribution of Serine Residues to Constitutive and Agonist-Induced Signaling via the D2S Dopamine Receptor: Evidence for Multiple, Agonist-Specific Active Conformations Mol. Pharmacol., August 1, 1998; 54(2): 435 - 444. [Abstract] [Full Text]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help