 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
Volume 17, Number 17, Issue of September 1, 1997 pp. 6647-6656
Copyright ©1997 Society for NeuroscienceAlteration of Ca2+ Dependence of Neurotransmitter Release by Disruption of Ca2+ Channel/Syntaxin Interaction
Received May 9, 1997; revised June 17, 1997; accepted June 20, 1997.
Jens Rettig1, Christian Heinemann1, Uri Ashery1, Zu-Hang Sheng2, Charles T. Yokoyama2, William A. Catterall2, and Erwin Neher1 1 Department of Membrane Biophysics, Max-Planck-Institute for Biophysical Chemistry, 37077 Göttingen, Germany, and 2 Department of Pharmacology, University of Washington, Seattle, Washington 98195-7280
Presynaptic N-type calcium channels interact with syntaxin and synaptosome-associated protein of 25 kDa (SNAP-25) through a binding site in the intracellular loop connecting domains II and III of the
1 subunit. This binding region was loaded into embryonic spinal neurons of Xenopus by early blastomere injection. After culturing, synaptic transmission of peptide-loaded and control cells was compared by measuring postsynaptic responses under different external Ca2+ concentrations. The relative transmitter release of injected neurons was reduced by ~25% at physiological Ca2+ concentration, whereas injection of the corresponding region of the L-type Ca2+ channel had virtually no effect. When applied to a theoretical model, these results imply that 70% of the formerly linked vesicles have been uncoupled after action of the peptide. Our data suggest that severing the physical interaction between presynaptic calcium channels and synaptic proteins will not prevent synaptic transmission at this synapse but will make it less efficient by shifting its Ca2+ dependence to higher values.
Key words: neuromuscular junction; Ca2+ dependence; synaptic transmission; calcium channel; synprint; syntaxin
This article has been cited by other articles:
Y. Shu, X. Liu, Y. Yang, M. Takahashi, and K. D. Gillis
Phosphorylation of SNAP-25 at Ser187 Mediates Enhancement of Exocytosis by a Phorbol Ester in INS-1 Cells
J. Neurosci., January 2, 2008; 28(1): 21 - 30.
[Abstract] [Full Text] [PDF]
D. D. Kline, A. Ramirez-Navarro, and D. L. Kunze
Adaptive Depression in Synaptic Transmission in the Nucleus of the Solitary Tract after In Vivo Chronic Intermittent Hypoxia: Evidence for Homeostatic Plasticity
J. Neurosci., April 25, 2007; 27(17): 4663 - 4673.
[Abstract] [Full Text] [PDF]
D. Singer-Lahat, A. Sheinin, D. Chikvashvili, S. Tsuk, D. Greitzer, R. Friedrich, L. Feinshreiber, U. Ashery, M. Benveniste, E. S. Levitan, et al.
K+ Channel Facilitation of Exocytosis by Dynamic Interaction with Syntaxin
J. Neurosci., February 14, 2007; 27(7): 1651 - 1658.
[Abstract] [Full Text] [PDF]
R. K. Keith, R. E. Poage, C. T. Yokoyama, W. A. Catterall, and S. D. Meriney
Bidirectional Modulation of Transmitter Release by Calcium Channel/Syntaxin Interactions In Vivo
J. Neurosci., January 10, 2007; 27(2): 265 - 269.
[Abstract] [Full Text] [PDF]
R. Cohen, B. M. Schmitt, and D. Atlas
Molecular Identification and Reconstitution of Depolarization-Induced Exocytosis Monitored by Membrane Capacitance
Biophys. J., December 1, 2005; 89(6): 4364 - 4373.
[Abstract] [Full Text] [PDF]
S. M. Cibulsky, H. Fei, and I. B. Levitan
Syntaxin-1A Binds to and Modulates the Slo Calcium-Activated Potassium Channel via an Interaction That Excludes Syntaxin Binding to Calcium Channels
J Neurophysiol, March 1, 2005; 93(3): 1393 - 1405.
[Abstract] [Full Text] [PDF]
S. Tsuk, I. Michaelevski, G. N. Bentley, R. H. Joho, D. Chikvashvili, and I. Lotan
Kv2.1 Channel Activation and Inactivation Is Influenced by Physical Interactions of Both Syntaxin 1A and the Syntaxin 1A/Soluble N-Ethylmaleimide-Sensitive Factor-25 (t-SNARE) Complex with the C Terminus of the Channel
Mol. Pharmacol., February 1, 2005; 67(2): 480 - 488.
[Abstract] [Full Text] [PDF]
A. B. Harkins, A. L. Cahill, J. F. Powers, A. S. Tischler, and A. P. Fox
Deletion of the synaptic protein interaction site of the N-type (CaV2.2) calcium channel inhibits secretion in mouse pheochromocytoma cells
PNAS, October 19, 2004; 101(42): 15219 - 15224.
[Abstract] [Full Text] [PDF]
Y. Su, R. J. Balice-Gordon, D. M. Hess, D. S. Landsman, J. Minarcik, J. Golden, I. Hurwitz, S. A. Liebhaber, and N. E. Cooke
Neurobeachin Is Essential for Neuromuscular Synaptic Transmission
J. Neurosci., April 7, 2004; 24(14): 3627 - 3636.
[Abstract] [Full Text] [PDF]
A. J. Merz and W. T. Wickner
Trans-SNARE interactions elicit Ca2+ efflux from the yeast vacuole lumen
J. Cell Biol., January 19, 2004; 164(2): 195 - 206.
[Abstract] [Full Text] [PDF]
E. A. Mathews, E. Garcia, C. M. Santi, G. P. Mullen, C. Thacker, D. G. Moerman, and T. P. Snutch
Critical Residues of the Caenorhabditis elegans unc-2 Voltage-Gated Calcium Channel That Affect Behavioral and Physiological Properties
J. Neurosci., July 23, 2003; 23(16): 6537 - 6545.
[Abstract] [Full Text] [PDF]
J. R. Coorssen, P. S. Blank, F. Albertorio, L. Bezrukov, I. Kolosova, X. Chen, P. S. Backlund Jr, and J. Zimmerberg
Regulated secretion: SNARE density, vesicle fusion and calcium dependence
J. Cell Sci., May 15, 2003; 116(10): 2087 - 2097.
[Abstract] [Full Text] [PDF]
B. K. Berdiev, J. Xia, L. A. McLean, J. M. Markert, G. Y. Gillespie, T. B. Mapstone, A. P. Naren, B. Jovov, J. K. Bubien, H.-L. Ji, et al.
Acid-sensing Ion Channels in Malignant Gliomas
J. Biol. Chem., April 18, 2003; 278(17): 15023 - 15034.
[Abstract] [Full Text] [PDF]
S. B. Condliffe, M. D. Carattino, R. A. Frizzell, and H. Zhang
Syntaxin 1A Regulates ENaC via Domain-specific Interactions
J. Biol. Chem., April 4, 2003; 278(15): 12796 - 12804.
[Abstract] [Full Text] [PDF]
G. Liu, N. Dilmac, N. Hilliard, and G. H. Hockerman
Cav1.3 Is Preferentially Coupled to Glucose-Stimulated Insulin Secretion in the Pancreatic beta -Cell Line INS-1
J. Pharmacol. Exp. Ther., April 1, 2003; 305(1): 271 - 278.
[Abstract] [Full Text]
S. Mochida, R. E. Westenbroek, C. T. Yokoyama, H. Zhong, S. J. Myers, T. Scheuer, K. Itoh, and W. A. Catterall
Requirement for the synaptic protein interaction site for reconstitution of synaptic transmission by P/Q-type calcium channels
PNAS, March 4, 2003; 100(5): 2819 - 2824.
[Abstract] [Full Text] [PDF]
J. Trommershauser, R. Schneggenburger, A. Zippelius, and E. Neher
Heterogeneous Presynaptic Release Probabilities: Functional Relevance for Short-Term Plasticity
Biophys. J., March 1, 2003; 84(3): 1563 - 1579.
[Abstract] [Full Text] [PDF]
J. D. Spafford, D. W. Munno, P. van Nierop, Z.-P. Feng, S. E. Jarvis, W. J. Gallin, A. B. Smit, G. W. Zamponi, and N. I. Syed
Calcium Channel Structural Determinants of Synaptic Transmission between Identified Invertebrate Neurons
J. Biol. Chem., January 31, 2003; 278(6): 4258 - 4267.
[Abstract] [Full Text] [PDF]
S. Kaneko, C. B. Cooper, N. Nishioka, H. Yamasaki, A. Suzuki, S. E. Jarvis, A. Akaike, M. Satoh, and G. W. Zamponi
Identification and Characterization of Novel Human Cav2.2 (alpha 1B) Calcium Channel Variants Lacking the Synaptic Protein Interaction Site
J. Neurosci., January 1, 2002; 22(1): 82 - 92.
[Abstract] [Full Text] [PDF]
T. Fergestad, M. N. Wu, K. L. Schulze, T. E. Lloyd, H. J. Bellen, and K. Broadie
Targeted Mutations in the Syntaxin H3 Domain Specifically Disrupt SNARE Complex Function in Synaptic Transmission
J. Neurosci., December 1, 2001; 21(23): 9142 - 9150.
[Abstract] [Full Text] [PDF]
B. T. Chen, M. V. Avshalumov, and M. E. Rice
H2O2 Is a Novel, Endogenous Modulator of Synaptic Dopamine Release
J Neurophysiol, June 1, 2001; 85(6): 2468 - 2476.
[Abstract] [Full Text] [PDF]
S. E. Jarvis and G. W. Zamponi
Distinct Molecular Determinants Govern Syntaxin 1A-Mediated Inactivation and G-Protein Inhibition of N-Type Calcium Channels
J. Neurosci., May 1, 2001; 21(9): 2939 - 2948.
[Abstract] [Full Text] [PDF]
G. T. Macleod, P. A. Dickens, and M. R. Bennett
Formation and Function of Synapses with Respect to Schwann Cells at the End of Motor Nerve Terminal Branches on Mature Amphibian (Bufo marinus) Muscle
J. Neurosci., April 1, 2001; 21(7): 2380 - 2392.
[Abstract] [Full Text] [PDF]
T. Sakaba and E. Neher
Quantitative Relationship between Transmitter Release and Calcium Current at the Calyx of Held Synapse
J. Neurosci., January 15, 2001; 21(2): 462 - 476.
[Abstract] [Full Text] [PDF]
B. A. Stewart, M. Mohtashami, W. S. Trimble, and G. L. Boulianne
SNARE proteins contribute to calcium cooperativity of synaptic transmission
PNAS, November 22, 2000; (2000) 250491397.
[Abstract] [Full Text]
R. C. Desai, B. Vyas, C. A. Earles, J. T. Littleton, J. A. Kowalchyck, T. F.J. Martin, and E.R. Chapman
The C2B Domain of Synaptotagmin Is a Ca2+-sensing Module Essential for Exocytosis
J. Cell Biol., September 5, 2000; 150(5): 1125 - 1136.
[Abstract] [Full Text] [PDF]
F. Kawasaki, R. Felling, and R. W. Ordway
A Temperature-Sensitive Paralytic Mutant Defines a Primary Synaptic Calcium Channel in Drosophila
J. Neurosci., July 1, 2000; 20(13): 4885 - 4889.
[Abstract] [Full Text] [PDF]
J. B. Bergsman and R. W. Tsien
Syntaxin Modulation of Calcium Channels in Cortical Synaptosomes As Revealed by Botulinum Toxin C1
J. Neurosci., June 15, 2000; 20(12): 4368 - 4378.
[Abstract] [Full Text] [PDF]
B. Dellinger, R. Felling, and R. W. Ordway
Genetic Modifiers of the Drosophila NSF Mutant, comatose, Include a Temperature-Sensitive Paralytic Allele of the Calcium Channel {alpha}1-Subunit Gene, cacophony
Genetics, May 1, 2000; 155(1): 203 - 211.
[Abstract] [Full Text]
A. P. Naren and K. L. Kirk
CFTR Chloride Channels: Binding Partners and Regulatory Networks
Physiology, April 1, 2000; 15(2): 57 - 61.
[Abstract] [Full Text] [PDF]
H. Grabsch, A. Pereverzev, M. Weiergräber, M. Schramm, M. Henry, R. Vajna, R. E. Beattie, S. G. Volsen, U. Klöckner, J. Hescheler, et al.
Immunohistochemical Detection of {alpha}1E Voltage-gated Ca2+ Channel Isoforms in Cerebellum, INS-1 Cells, and Neuroendocrine Cells of the Digestive System
J. Histochem. Cytochem., August 1, 1999; 47(8): 981 - 994.
[Abstract] [Full Text]
R. E. Poage, S. D. Meriney, C. B. Gundersen, and J. A. Umbach
Antibodies Against Cysteine String Proteins Inhibit Evoked Neurotransmitter Release at Xenopus Neuromuscular Junctions
J Neurophysiol, July 1, 1999; 82(1): 50 - 59.
[Abstract] [Full Text] [PDF]
A. Meir, S. Ginsburg, A. Butkevich, S. G. Kachalsky, I. Kaiserman, R. Ahdut, S. Demirgoren, and R. Rahamimoff
Ion Channels in Presynaptic Nerve Terminals and Control of Transmitter Release
Physiol Rev, July 1, 1999; 79(3): 1019 - 1088.
[Abstract] [Full Text] [PDF]
U. Ashery, H. Koch, V. Scheuss, N. Brose, and J. Rettig
A presynaptic role for the ADP ribosylation factor (ARF)-specific GDP/GTP exchange factor msec7-1
PNAS, February 2, 1999; 96(3): 1094 - 1099.
[Abstract] [Full Text] [PDF]
L.-G. Wu, R. E. Westenbroek, J. G. G. Borst, W. A. Catterall, and B. Sakmann
Calcium Channel Types with Distinct Presynaptic Localization Couple Differentially to Transmitter Release in Single Calyx-Type Synapses
J. Neurosci., January 15, 1999; 19(2): 726 - 736.
[Abstract] [Full Text] [PDF]
O. Wiser, M. Trus, A. Hernandez, E. Renstrom, S. Barg, P. Rorsman, and D. Atlas
The voltage sensitive Lc-type Ca2+ channel is functionally coupled to the exocytotic machinery
PNAS, January 5, 1999; 96(1): 248 - 253.
[Abstract] [Full Text] [PDF]
J. R. Coorssen, P. S. Blank, M. Tahara, and J. Zimmerberg
Biochemical and Functional Studies of Cortical Vesicle Fusion: The SNARE Complex and Ca2+ Sensitivity
J. Cell Biol., December 28, 1998; 143(7): 1845 - 1857.
[Abstract] [Full Text] [PDF]
E. R. Chapman, R. C. Desai, A. F. Davis, and C. K. Tornehl
Delineation of the Oligomerization, AP-2 Binding, and Synprint Binding Region of the C2B Domain of Synaptotagmin
J. Biol. Chem., December 4, 1998; 273(49): 32966 - 32972.
[Abstract] [Full Text] [PDF]
S. Mochida, C. T. Yokoyama, D. K. Kim, K. Itoh, and W. A. Catterall
Evidence for a voltage-dependent enhancement of neurotransmitter release mediated via the synaptic protein interaction site of N-type Ca2+ channels
PNAS, November 24, 1998; 95(24): 14523 - 14528.
[Abstract] [Full Text] [PDF]
C. Leveque, S. Pupier, B. Marqueze, L. Geslin, M. Kataoka, M. Takahashi, M. De Waard, and M. Seagar
Interaction of Cysteine String Proteins with the alpha 1A Subunit of the P/Q-type Calcium Channel
J. Biol. Chem., May 29, 1998; 273(22): 13488 - 13492.
[Abstract] [Full Text] [PDF]
R. Ranjan, P. Bronk, and K. E. Zinsmaier
Cysteine String Protein Is Required for Calcium Secretion Coupling of Evoked Neurotransmission in Drosophila But Not for Vesicle Recycling
J. Neurosci., February 1, 1998; 18(3): 956 - 964.
[Abstract] [Full Text] [PDF]
D. K. Kim and W. A. Catterall
Ca2+-dependent and -independent interactions of the isoforms of the alpha 1A subunit of brain Ca2+ channels with presynaptic SNARE proteins
PNAS, December 23, 1997; 94(26): 14782 - 14786.
[Abstract] [Full Text] [PDF]
C. von Poser, J. Z. Zhang, C. Mineo, W. Ding, Y. Ying, T. C. Sudhof, and R. G. W. Anderson
Synaptotagmin Regulation of Coated Pit Assembly
J. Biol. Chem., September 29, 2000; 275(40): 30916 - 30924.
[Abstract] [Full Text] [PDF]
B. A. Stewart, M. Mohtashami, W. S. Trimble, and G. L. Boulianne
SNARE proteins contribute to calcium cooperativity of synaptic transmission
PNAS, December 5, 2000; 97(25): 13955 - 13960.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|