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Volume 17, Number 19,
Issue of October 1, 1997
pp. 7210-7219
Copyright ©1997 Society for Neuroscience
Rapid Synaptic Transmission in the Avian Ciliary Ganglion Is
Mediated by Two Distinct Classes of Nicotinic Receptors
Received May 6, 1997; revised July 8, 1997; accepted July 10, 1997.
Erik M. Ullian1,
J. Michael McIntosh2, and
Peter B. Sargent1
1 Neuroscience Graduate Program and Departments of
Stomatology and Physiology, University of California, San Francisco,
California 94143, and 2 Departments of Psychiatry and
Biology, University of Utah, Salt Lake City, Utah 84112
We analyzed the kinetics and pharmacology of EPSCs in two kinds of
neurons in the embryonic avian ciliary ganglion. Whole-cell voltage-clamp recordings revealed that the singly innervated ciliary neurons had large-amplitude (1.5-8.0 nA) EPSCs that could be
classified according to the kinetics of their falling phases. Most of
the neurons responded with an EPSC the falling phase of which followed a double exponential time course with time constants of ~1 and 10 msec. The EPSCs of the remaining ciliary neurons followed a single time
constant (~8 msec). Multiple innervated choroid neurons had
smaller-amplitude responses (0.2-1.5 nA when all inputs were activated) that appeared to contain only a slowly decaying component ( = 12 msec). The fast and slow components of EPSC decay seen in
most ciliary neurons could be pharmacologically isolated with two
toxins against nicotinic acetylcholine receptors (AChRs). The fast
component was blocked by 50 nM  -bungarotoxin (-BuTx), which binds 7-subunit-containing AChRs. The slow component was selectively blocked by 50 nM -conotoxin MII
(-CTx-MII), which blocks mammalian AChRs containing an 3/ 2
subunit interface. A combination of both -BuTx and -CTx-MII
abolished nearly all evoked current. Similar pharmacological results
were found for ciliary neurons with monoexponentially decaying EPSCs
and for choroid neurons. These results suggest that nerve-evoked
transmitter acts on at least two different populations of AChRs on
autonomic motor neurons in the ciliary ganglion.
Key words:
acetylcholine receptors;
excitatory postsynaptic current;
-bungarotoxin;
-conotoxin MII;
7;
3;
2
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