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Volume 17, Number 2, Issue of January 15, 1997 pp. 834-842
Copyright ©1997 Society for Neuroscience

Differential Localization of  Glutamate Receptors in the Rat Cerebellum: Coexpression with AMPA Receptors in Parallel Fiber-Spine Synapses and Absence from Climbing Fiber-Spine Synapses

Received Sept. 3, 1996; revised Oct. 23, 1996; accepted Nov. 5, 1996.

Alf Sommer Landsend¹, Mahmood Amiry-Moghaddam¹, Atsushi Matsubara², Linda Bergersen¹, Shin-ichi Usami², Robert J. Wenthold³, and Ole P. Ottersen¹

¹ Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, N-0317 Oslo, Norway, ² Department of Otorhinolaryngology, Hirosaki University School of Medicine, Hirosaki 036, Japan, and ³ Laboratory of Neurochemistry, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, Maryland 20892

The 2 glutamate receptors are prominently expressed in Purkinje cells and are thought to play a key role in the induction of cerebellar long-term depression. The synaptic and subsynaptic localization of  receptors in rat cerebellar cortex was investigated with sensitive and high-resolution immunogold procedures. After postembedding incubation with an antibody raised to a C-terminal peptide of 2, high gold particle densities occurred in all parallel fiber synapses with Purkinje cell dendritic spines, whereas other synapses were consistently devoid of labeling. Among the types of immunonegative synapse were climbing fiber synapses with spines and parallel fiber synapses with dendritic stems of interneurons. At the parallel fiber-spine synapse, gold particles signaling  receptors were restricted to the postsynaptic specialization. By the use of double labeling with two different gold particle sizes, it was shown that  and AMPA GluR2/3 receptors were colocalized along the entire extent of the postsynaptic specialization without forming separate domains. The distribution of gold particles representing  receptors was consistent with a cytoplasmic localization of the C terminus and an absence of a significant presynaptic pool of receptor molecules. The present data suggest that the 2 receptors are targeted selectively to a subset of Purkinje cell spines and that they are coexpressed with ionotropic receptors in the postsynaptic specialization. This arrangement could allow for a direct interaction between the two classes of receptor.

Key words: immunogold; glutamate receptor; freeze substitution; colocalization; synapse; cerebellum

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