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Volume 17, Number 20, Issue of October 15, 1997 pp. 8049-8060
Copyright ©1997 Society for Neuroscience

Inflammation Increases the Distribution of Dorsal Horn Neurons That Internalize the Neurokinin-1 Receptor in Response to Noxious and Non-Noxious Stimulation

Received June 9, 1997; revised July 30, 1997; accepted Aug. 6, 1997.

Catherine Abbadie1, Jodie Trafton1, 2, Hantao Liu1, Patrick W. Mantyh3, and Allan I. Basbaum1

1 Departments of Anatomy and Physiology, W. M. Keck Foundation for Integrative Neuroscience, and 2 Program in Neurosciences, University of California San Francisco, San Francisco, California 94143, and 3 Molecular Neurobiology Laboratory, Veterans Administration Medical Center, Minneapolis, Minnesota 55417

Although the neurokinin-1 (NK-1)/substance P (SP) receptor is expressed by neurons throughout the spinal dorsal horn, noxious chemical stimulation in the normal rat only induces internalization of the receptor in cell bodies and dendrites of lamina I. Here we compared the effects of mechanical and thermal stimulation in normal rats and in rats with persistent hindpaw inflammation. Electron microscopic analysis confirmed the upregulation of receptor that occurs with inflammation and demonstrated that in the absence of superimposed stimulation, the increased receptor was, as in normal rats, concentrated on the plasma membrane. In general, noxious mechanical was more effective than noxious thermal stimulation in inducing NK-1 receptor internalization, and this was increased in the setting of inflammation. Although a 5 sec noxious mechanical stimulus only induced internalization in 22% of lamina I neurons in normal rats, after inflammation, it evoked near-maximal (98%) internalization in lamina I, produced significant changes in laminae III-VI, and expanded the rostrocaudal distribution of neurons with internalized receptor. Even non-noxious (brush) stimulation of the inflamed hindpaw induced internalization in large numbers of superficial and deep neurons. For thermal stimulation, the percentage of cells with internalized receptor increased linearly at >45°C, but in normal rats, these were restricted to lamina I. After inflammation, however, the 52°C stimulus also induced internalization in 25% of laminae III-IV cells. These studies provide a new perspective on the reorganization of dorsal horn circuits in the setting of persistent injury and demonstrate a critical contribution of SP.

Key words: allodynia; dorsal horn; hyperalgesia; inflammation; neurokinin; substance P




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