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Volume 17, Number 23, Issue of December 1, 1997 pp. 9183-9193

Neuronal and Non-Neuronal Collapsin-1 Binding Sites in Developing Chick Are Distinct from Other Semaphorin Binding Sites

Received June 13, 1997; revised Sept. 2, 1997; accepted Sept. 11, 1997.

Takuya Takahashi^{1, 2}, Fumio Nakamura¹, and Stephen M. Strittmatter^{1, 3}

Departments of ¹ Neurology, ² Biology, and ³ Neurobiology, Yale University, New Haven, Connecticut 06520

The collapsin and semaphorin family of extracellular proteins contributes to axonal path finding by repulsing axons and collapsing growth cones. To explore the mechanism of collapsin-1 action, we expressed and purified a truncated collapsin-1-alkaline phosphatase fusion protein (CAP-4). This protein retains biological activity as a DRG growth cone collapsing agent and saturably binds to DRG neurons with low nanomolar affinity. Specific CAP-4 binding sites are present on DRG neurons, sympathetic neurons, and motoneurons, but not on retinal, cortical, or brainstem neurons. Outside the nervous system, high levels of CAP-4 binding sites are present in the mesenchyme surrounding major blood vessels and developing bone and in lung. These sites provide a substrate for the collapsin-1-dependent patterning of non-neuronal tissues perturbed in sema III (/) mice. The staining patterns for mouse semaphorin D/III and chick collapsin-1 fusion proteins are indistinguishable from one another but quite separate from that for semaphorin B and M-semaphorin F fusion proteins. These data imply that a family of high-affinity semaphorin binding sites similar in complexity to the semaphorin ligand family exists.

Key words: collapsin; semaphorin; growth cone collapse; dorsal root ganglion; sympathetic neuron; alkaline phosphatase

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