 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
Volume 17, Number 24, Issue of December 15, 1997 pp. 9554-9564
Measurement of Intracellular Free Zinc in Living Cortical Neurons: Routes of Entry
Received May 15, 1997; revised Sept. 26, 1997; accepted Sept. 30, 1997.
Stefano L. Sensi, Lorella M. T. Canzoniero, Shan Ping Yu, Howard S. Ying, Jae-Young Koh, Geoffrey A. Kerchner, and Dennis W. Choi Center for the Study of Nervous System Injury and Department of Neurology, Washington University School of Medicine, St. Louis, Missouri 63110
We used the ratioable fluorescent dye mag-fura-5 to measure intracellular free Zn2+ ([Zn2+]i) in cultured neocortical neurons exposed to neurotoxic concentrations of Zn2+ in concert with depolarization or glutamate receptor activation and identified four routes of Zn2+ entry. Neurons exposed to extracellular Zn2+ plus high K+ responded with a peak cell body signal corresponding to a [Zn2+]i of 35-45 nM. This increase in [Zn2+]i was attenuated by concurrent addition of Gd3+, verapamil,
-conotoxin GVIA, or nimodipine, consistent with Zn2+ entry through voltage-gated Ca2+channels. Furthermore, under conditions favoring reverse operation of the Na+-Ca2+ exchanger, Zn2+ application induced a slow increase in [Zn2+]i and outward whole-cell current sensitive to benzamil-amiloride. Thus, a second route of Zn2+ entry into neurons may be via transporter-mediated exchange with intracellular Na+. Both NMDA and kainate also induced rapid increases in neuronal [Zn2+]i. The NMDA-induced increase was only partly sensitive to Gd3+ or to removal of extracellular Na+, consistent with a third route of entry directly through NMDA receptor-gated channels. The kainate-induced increase was highly sensitive to Gd3+ or Na+ removal in most neurons but insensitive in a minority subpopulation ("cobalt-positive cells"), suggesting that a fourth route of neuronal Zn2+ entry is through the Ca2+-permeable channels gated by certain subtypes of AMPA or kainate receptors.
Key words: mag-fura-5; voltage-gated calcium channels; sodium-calcium exchanger; calcium; sodium; glutamate; AMPA; NMDA; neurotoxicity; global ischemia; hypoxia
This article has been cited by other articles:
B. Morgan, S. K. Ang, G. Yan, and H. Lu
Zinc Can Play Chaperone-like and Inhibitor Roles during Import of Mitochondrial Small Tim Proteins
J. Biol. Chem., March 13, 2009; 284(11): 6818 - 6825.
[Abstract] [Full Text] [PDF]
B. M. Krenn, E. Gaudernak, B. Holzer, K. Lanke, F. J. M. Van Kuppeveld, and J. Seipelt
Antiviral Activity of the Zinc Ionophores Pyrithione and Hinokitiol against Picornavirus Infections
J. Virol., January 1, 2009; 83(1): 58 - 64.
[Abstract] [Full Text] [PDF]
T. H. Evers, M. A.M. Appelhof, E.W. Meijer, and M. Merkx
His-tags as Zn(II) binding motifs in a protein-based fluorescent sensor
Protein Eng. Des. Sel., August 1, 2008; 21(8): 529 - 536.
[Abstract] [Full Text] [PDF]
A. V. Gyulkhandanyan, H. Lu, S. C. Lee, A. Bhattacharjee, N. Wijesekara, J. E. M. Fox, P. E. MacDonald, F. Chimienti, F. F. Dai, and M. B. Wheeler
Investigation of Transport Mechanisms and Regulation of Intracellular Zn2+ in Pancreatic {alpha}-Cells
J. Biol. Chem., April 11, 2008; 283(15): 10184 - 10197.
[Abstract] [Full Text] [PDF]
R. A. Colvin, A. I. Bush, I. Volitakis, C. P. Fontaine, D. Thomas, K. Kikuchi, and W. R. Holmes
Insights into Zn2+ homeostasis in neurons from experimental and modeling studies
Am J Physiol Cell Physiol, March 1, 2008; 294(3): C726 - C742.
[Abstract] [Full Text] [PDF]
A. V. Gyulkhandanyan, S. C. Lee, G. Bikopoulos, F. Dai, and M. B. Wheeler
The Zn2+-transporting Pathways in Pancreatic beta-Cells: A ROLE FOR THE L-TYPE VOLTAGE-GATED Ca2+ CHANNEL
J. Biol. Chem., April 7, 2006; 281(14): 9361 - 9372.
[Abstract] [Full Text] [PDF]
L. M. Malaiyandi, A. S. Honick, G. L. Rintoul, Q. J. Wang, and I. J. Reynolds
Zn2+ Inhibits Mitochondrial Movement in Neurons by Phosphatidylinositol 3-Kinase Activation
J. Neurosci., October 12, 2005; 25(41): 9507 - 9514.
[Abstract] [Full Text] [PDF]
A. Cote, M. Chiasson, M. R Peralta III, K. Lafortune, L. Pellegrini, and K. Toth
Cell type-specific action of seizure-induced intracellular zinc accumulation in the rat hippocampus
J. Physiol., August 1, 2005; 566(3): 821 - 837.
[Abstract] [Full Text] [PDF]
J. J. Hwang, M.-H. Park, S.-Y. Choi, and J.-Y. Koh
Activation of the Trk Signaling Pathway by Extracellular Zinc: ROLE OF METALLOPROTEINASES
J. Biol. Chem., March 25, 2005; 280(12): 11995 - 12001.
[Abstract] [Full Text] [PDF]
H. Azriel-Tamir, H. Sharir, B. Schwartz, and M. Hershfinkel
Extracellular Zinc Triggers ERK-dependent Activation of Na+/H+ Exchange in Colonocytes Mediated by the Zinc-sensing Receptor
J. Biol. Chem., December 10, 2004; 279(50): 51804 - 51816.
[Abstract] [Full Text] [PDF]
T. G. Smart, A. M. Hosie, and P. S. Miller
Zn2+ Ions: Modulators of Excitatory and Inhibitory Synaptic Activity
Neuroscientist, October 1, 2004; 10(5): 432 - 442.
[Abstract] [PDF]
J.-Y. Im, D. Kim, K.-W. Lee, J.-B. Kim, J.-K. Lee, D. S. Kim, Y. I. Lee, K.-S. Ha, C. O Joe, and P.-L. Han
COX-2 Regulates the Insulin-Like Growth Factor I-Induced Potentiation of Zn2+-Toxicity in Primary Cortical Culture
Mol. Pharmacol., September 1, 2004; 66(3): 368 - 376.
[Abstract] [Full Text] [PDF]
A.-L. Prost, A. Bloc, N. Hussy, R. Derand, and M. Vivaudou
Zinc is both an intracellular and extracellular regulator of KATP channel function
J. Physiol., August 15, 2004; 559(1): 157 - 167.
[Abstract] [Full Text] [PDF]
A. Gore, A. Moran, M. Hershfinkel, and I. Sekler
Inhibitory Mechanism of Store-operated Ca2+ Channels by Zinc
J. Biol. Chem., March 19, 2004; 279(12): 11106 - 11111.
[Abstract] [Full Text] [PDF]
J. Jeong, S. Suh, C. Guan, Y.-F. Tsay, N. Moran, C. J. Oh, C. S. An, K. N. Demchenko, K. Pawlowski, and Y. Lee
A Nodule-Specific Dicarboxylate Transporter from Alder Is a Member of the Peptide Transporter Family
Plant Physiology, March 1, 2004; 134(3): 969 - 978.
[Abstract] [Full Text] [PDF]
E. Ohana, D. Segal, R. Palty, D. Ton-That, A. Moran, S. L. Sensi, J. H. Weiss, M. Hershfinkel, and I. Sekler
A Sodium Zinc Exchange Mechanism Is Mediating Extrusion of Zinc in Mammalian Cells
J. Biol. Chem., February 6, 2004; 279(6): 4278 - 4284.
[Abstract] [Full Text] [PDF]
C. J. Frederickson, W. Maret, and M. P. Cuajungco
Zinc and Excitotoxic Brain Injury: A New Model
Neuroscientist, February 1, 2004; 10(1): 18 - 25.
[Abstract] [PDF]
R. H. Dyck, A. Chaudhuri, and M. S. Cynader
Experience-dependent Regulation of the Zincergic Innervation of Visual Cortex in Adult Monkeys
Cereb Cortex, October 1, 2003; 13(10): 1094 - 1109.
[Abstract] [Full Text] [PDF]
D. J. Eide
Multiple Regulatory Mechanisms Maintain Zinc Homeostasis in Saccharomyces cerevisiae
J. Nutr., May 1, 2003; 133(5): 1532S - 1535.
[Abstract] [Full Text] [PDF]
C. J. Frederickson, S. W. Suh, J.-Y. Koh, Y. K. Cha, R. B. Thompson, C. J. LaBuda, R. V. Balaji, and M. P. Cuajungco
Depletion of Intracellular Zinc from Neurons by Use of an Extracellular Chelator In Vivo and In Vitro
J. Histochem. Cytochem., December 1, 2002; 50(12): 1659 - 1662.
[Abstract] [Full Text] [PDF]
C. W. MacDiarmid, M. A. Milanick, and D. J. Eide
Biochemical Properties of Vacuolar Zinc Transport Systems of Saccharomyces cerevisiae
J. Biol. Chem., October 11, 2002; 277(42): 39187 - 39194.
[Abstract] [Full Text] [PDF]
K. E. Dineley, L. M. Malaiyandi, and I. J. Reynolds
A Reevaluation of Neuronal Zinc Measurements: Artifacts Associated with High Intracellular Dye Concentration
Mol. Pharmacol., September 1, 2002; 62(3): 618 - 627.
[Abstract] [Full Text] [PDF]
Y. Jia, J.-M. Jeng, S. L Sensi, and J. H Weiss
Zn2+ currents are mediated by calcium-permeable AMPA/Kainate channels in cultured murine hippocampal neurones
J. Physiol., August 15, 2002; 543(1): 35 - 48.
[Abstract] [Full Text] [PDF]
S. Ueno, M. Tsukamoto, T. Hirano, K. Kikuchi, M. K. Yamada, N. Nishiyama, T. Nagano, N. Matsuki, and Y. Ikegaya
Mossy fiber Zn2+ spillover modulates heterosynaptic N-methyl-D-aspartate receptor activity in hippocampal CA3 circuits
J. Cell Biol., July 22, 2002; 158(2): 215 - 220.
[Abstract] [Full Text] [PDF]
H. Z. Yin, S. L. Sensi, F. Ogoshi, and J. H. Weiss
Blockade of Ca2+-Permeable AMPA/Kainate Channels Decreases Oxygen-Glucose Deprivation-Induced Zn2+ Accumulation and Neuronal Loss in Hippocampal Pyramidal Neurons
J. Neurosci., February 15, 2002; 22(4): 1273 - 1279.
[Abstract] [Full Text] [PDF]
P. Manzerra, M. M. Behrens, L. M. T. Canzoniero, X. Q. Wang, V. Heidinger, T. Ichinose, S. P. Yu, and D. W. Choi
Zinc induces a Src family kinase-mediated up-regulation of NMDA receptor activity and excitotoxicity
PNAS, September 25, 2001; 98(20): 11055 - 11061.
[Abstract] [Full Text] [PDF]
W. H. Yu and P. E. Fraser
S100{beta} Interaction with Tau Is Promoted by Zinc and Inhibited by Hyperphosphorylation in Alzheimer's Disease
J. Neurosci., April 1, 2001; 21(7): 2240 - 2246.
[Abstract] [Full Text] [PDF]
D. D. Lin, A. S. Cohen, and D. A. Coulter
Zinc-Induced Augmentation of Excitatory Synaptic Currents and Glutamate Receptor Responses in Hippocampal CA3 Neurons
J Neurophysiol, March 1, 2001; 85(3): 1185 - 1196.
[Abstract] [Full Text] [PDF]
D. Ragozzino, A. Giovannelli, V. Degasperi, F. Eusebi, and F. Grassi
Zinc permeates mouse muscle ACh receptor channels expressed in BOSC 23 cells and affects channel function
J. Physiol., November 15, 2000; 529(1): 83 - 91.
[Abstract] [Full Text] [PDF]
G. A Kerchner, L. M T Canzoniero, S. P. Yu, C. Ling, and D. W Choi
Zn2+ current is mediated by voltage-gated Ca2+ channels and enhanced by extracellular acidity in mouse cortical neurones
J. Physiol., October 1, 2000; 528(1): 39 - 52.
[Abstract] [Full Text] [PDF]
C. T. Sheline, M. M. Behrens, and D. W. Choi
Zinc-Induced Cortical Neuronal Death: Contribution of Energy Failure Attributable to Loss of NAD+ and Inhibition of Glycolysis
J. Neurosci., May 1, 2000; 20(9): 3139 - 3146.
[Abstract] [Full Text] [PDF]
R. A. Colvin, N. Davis, R. W. Nipper, and P. A. Carter
Zinc Transport in the Brain: Routes of Zinc Influx and Efflux in Neurons
J. Nutr., May 1, 2000; 130(5): 1484S - 1487.
[Abstract] [Full Text]
A. M. Brown, B. S. Kristal, M. S. Effron, A. I. Shestopalov, P. A. Ullucci, K.-F. R. Sheu, J. P. Blass, and A. J. L. Cooper
Zn2+ Inhibits alpha -Ketoglutarate-stimulated Mitochondrial Respiration and the Isolated alpha -Ketoglutarate Dehydrogenase Complex
J. Biol. Chem., April 28, 2000; 275(18): 13441 - 13447.
[Abstract] [Full Text] [PDF]
W. L. Erdahl, C. J. Chapman, R. W. Taylor, and D. R. Pfeiffer
Ionomycin, a Carboxylic Acid Ionophore, Transports Pb2+ with High Selectivity
J. Biol. Chem., March 15, 2000; 275(10): 7071 - 7079.
[Abstract] [Full Text] [PDF]
H. H. Sandstead, C. J. Frederickson, and J. G. Penland
History of Zinc as Related to Brain Function
J. Nutr., February 1, 2000; 130(2): 496 - 496.
[Abstract] [Full Text]
M. Alirezaei, A. C. Nairn, J. Glowinski, J. Premont, and P. Marin
Zinc Inhibits Protein Synthesis in Neurons. POTENTIAL ROLE OF PHOSPHORYLATION OF TRANSLATION INITIATION FACTOR-2alpha
J. Biol. Chem., November 5, 1999; 274(45): 32433 - 32438.
[Abstract] [Full Text] [PDF]
M. van Lookeren Campagne, H. Thibodeaux, N. van Bruggen, B. Cairns, R. Gerlai, J. T. Palmer, S. P. Williams, and D. G. Lowe
Evidence for a protective role of metallothionein-1 in focal cerebral ischemia
PNAS, October 26, 1999; 96(22): 12870 - 12875.
[Abstract] [Full Text] [PDF]
S. I. Savitz and D. M. Rosenbaum
Review : Gene Expression after Cerebral Ischemia
Neuroscientist, July 1, 1999; 5(4): 238 - 253.
[Abstract] [PDF]
T. Tabata and A. T. Ishida
A Zinc-Dependent Cl- Current in Neuronal Somata
J. Neurosci., July 1, 1999; 19(13): 5195 - 5204.
[Abstract] [Full Text] [PDF]
Y. Kim, J. Park, S. H. Hong, and J. Koh
Nonproteolytic Neuroprotection by Human Recombinant Tissue Plasminogen Activator
Science, April 23, 1999; 284(5414): 647 - 650.
[Abstract] [Full Text]
S. L. Sensi, H. Z. Yin, S. G. Carriedo, S. S. Rao, and J. H. Weiss
Preferential Zn2+ influx through Ca2+-permeable AMPA/kainate channels triggers prolonged mitochondrial superoxide production
PNAS, March 2, 1999; 96(5): 2414 - 2419.
[Abstract] [Full Text] [PDF]
R. Dingledine, K. Borges, D. Bowie, and S. F. Traynelis
The Glutamate Receptor Ion Channels
Pharmacol. Rev., March 1, 1999; 51(1): 7 - 62.
[Abstract] [Full Text] [PDF]
T. B. Cole, H. J. Wenzel, K. E. Kafer, P. A. Schwartzkroin, and R. D. Palmiter
Elimination of zinc from synaptic vesicles in the intact mouse brain by disruption of the ZnT3 gene
PNAS, February 16, 1999; 96(4): 1716 - 1721.
[Abstract] [Full Text] [PDF]
E. Palma, L. Maggi, R. Miledi, and F. Eusebi
Effects of Zn2+ on wild and mutant neuronal alpha 7 nicotinic receptors
PNAS, August 18, 1998; 95(17): 10246 - 10250.
[Abstract] [Full Text] [PDF]
H. S. Ying, J. H. Weishaupt, M. Grabb, L. M. T. Canzoniero, S. L. Sensi, C. T. Sheline, H. Monyer, and D. W. Choi
Sublethal Oxygen-Glucose Deprivation Alters Hippocampal Neuronal AMPA Receptor Expression and Vulnerability to Kainate-Induced Death
J. Neurosci., December 15, 1997; 17(24): 9536 - 9544.
[Abstract] [Full Text] [PDF]
C. E. Outten and T. V. O'Halloran
Femtomolar Sensitivity of Metalloregulatory Proteins Controlling Zinc Homeostasis
Science, June 29, 2001; 292(5526): 2488 - 2492.
[Abstract] [Full Text] [PDF]
L. M. T. Canzoniero, D. M. Turetsky, and D. W. Choi
Measurement of Intracellular Free Zinc Concentrations Accompanying Zinc-Induced Neuronal Death
J. Neurosci., October 1, 1999; 19(19): RC31 - RC31.
[Abstract] [Full Text] [PDF]
R. A. Colvin
pH dependence and compartmentalization of zinc transported across plasma membrane of rat cortical neurons
Am J Physiol Cell Physiol, February 1, 2002; 282(2): C317 - C329.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|