 |
 Previous Article | Next Article 
Volume 17, Number 3,
Issue of February 1, 1997
pp. 975-982
Copyright ©1997 Society for Neuroscience
Tumor Necrosis Factor Enhances the Capsaicin Sensitivity of Rat
Sensory Neurons
Received Oct. 31, 1996; accepted Nov. 18, 1996.
Grant D. Nicol1,
John
C. Lopshire2, and
Carl M. Pafford2
1 Department of Pharmacology and Toxicology and
2 Medical Neurobiology Program, School of Medicine, Indiana
University, Indianapolis, Indiana 46202-5120
The capacity of the proinflammatory cytokines, tumor necrosis
factor  (TNF) and interleukin 1 (IL-1), to modulate the sensitivity of isolated sensory neurons grown in culture to the excitatory chemical agent capsaicin was examined. Alterations in
capsaicin sensitivity were assessed by quantifying the number of
neurons labeled with cobalt after exposure to capsaicin and by
recording the whole-cell response from a single neuron to the focal
application of capsaicin. A 24 hr pretreatment of the neuronal cultures
with TNF (10 or 50 ng/ml), but not IL-1 (10 or 50 ng/ml), produced a concentration-dependent increase in the number of
cobalt-labeled neurons after exposure to 100 nM capsaicin.
The peak increase in the number of labeled neurons was attained after a
4 hr treatment with 10 ng/ml TNF. Similarly, pretreatment with
TNF (10 ng/ml for 4, 12, and 24 hr) produced a greater than twofold
increase in the average peak amplitude of the inward current evoked by 100 nM capsaicin. Both the TNF-induced increase in
labeling and current amplitude were blocked by treating the neuronal
cultures with indomethacin before the addition of TNF. Enhancement
of the capsaicin-evoked current also was blocked by the specific cyclo-oxygenase-2 inhibitor SC-236. These results indicate that TNF
can enhance the sensitivity of sensory neurons to the excitation produced by capsaicin and that this enhancement likely is mediated by
the neuronal production of prostaglandins. Isolated sensory neurons
grown in culture may prove to be a useful model system in which to
explore how prolonged exposure to mediators associated with chronic
inflammation alter the regulatory pathways that modulate the
excitability of the nervous system.
Key words:
tumor necrosis factor ;
interleukin 1;
capsaicin;
sensitization;
prostaglandins;
cyclo-oxygenase-2;
membrane
excitability
This article has been cited by other articles:
|
|
|
|
 
H. Jia, X. Feng, W. Li, Y. Hu, Q. Zeng, J. Liu, and J. Xu
Recombinant Human Erythropoietin Attenuates Spinal Neuroimmune Activation of Neuropathic Pain in Rats
Ann. Clin. Lab. Sci.,
January 1, 2009;
39(1):
84 - 91.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kawasaki, L. Zhang, J.-K. Cheng, and R.-R. Ji
Cytokine Mechanisms of Central Sensitization: Distinct and Overlapping Role of Interleukin-1{beta}, Interleukin-6, and Tumor Necrosis Factor-{alpha} in Regulating Synaptic and Neuronal Activity in the Superficial Spinal Cord
J. Neurosci.,
May 14, 2008;
28(20):
5189 - 5194.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. E. Constantin, N. Mair, C. A. Sailer, M. Andratsch, Z.-Z. Xu, M. J. F. Blumer, N. Scherbakov, J. B. Davis, H. Bluethmann, R.-R. Ji, et al.
Endogenous Tumor Necrosis Factor {alpha} (TNF{alpha}) Requires TNF Receptor Type 2 to Generate Heat Hyperalgesia in a Mouse Cancer Model
J. Neurosci.,
May 7, 2008;
28(19):
5072 - 5081.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. A. White, H. Jung, and R. J. Miller
Chemokines and the pathophysiology of neuropathic pain
PNAS,
December 18, 2007;
104(51):
20151 - 20158.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Zhang, Y. Chen, C. Wang, and L.-Y. M. Huang
Neuronal somatic ATP release triggers neuron-satellite glial cell communication in dorsal root ganglia
PNAS,
June 5, 2007;
104(23):
9864 - 9869.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M Hartel, F F di Mola, F Selvaggi, G Mascetta, M N Wente, K Felix, N A Giese, U Hinz, P Di Sebastiano, M W Buchler, et al.
Vanilloids in pancreatic cancer: potential for chemotherapy and pain management
Gut,
April 1, 2006;
55(4):
519 - 528.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Liu, T. M. Yang, W. Liedtke, and S. A. Simon
Chronic IL-1beta Signaling Potentiates Voltage-Dependent Sodium Currents in Trigeminal Nociceptive Neurons
J Neurophysiol,
March 1, 2006;
95(3):
1478 - 1490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Jin and R. W. Gereau IV
Acute p38-Mediated Modulation of Tetrodotoxin-Resistant Sodium Channels in Mouse Sensory Neurons by Tumor Necrosis Factor-{alpha}
J. Neurosci.,
January 4, 2006;
26(1):
246 - 255.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. B. Veldhuis, M. van der Stelt, M. W. Wadman, G. van Zadelhoff, M. Maccarrone, F. Fezza, G. A. Veldink, J. F. G. Vliegenthart, P. R. Bar, K. Nicolay, et al.
Neuroprotection by the Endogenous Cannabinoid Anandamide and Arvanil against In Vivo Excitotoxicity in the Rat: Role of Vanilloid Receptors and Lipoxygenases
J. Neurosci.,
May 15, 2003;
23(10):
4127 - 4133.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Schafers, D. H. Lee, D. Brors, T. L. Yaksh, and L. S. Sorkin
Increased Sensitivity of Injured and Adjacent Uninjured Rat Primary Sensory Neurons to Exogenous Tumor Necrosis Factor-alpha after Spinal Nerve Ligation
J. Neurosci.,
April 1, 2003;
23(7):
3028 - 3038.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-M. Zhang, H. Li, B. Liu, and S. J. Brull
Acute Topical Application of Tumor Necrosis Factor alpha Evokes Protein Kinase A-Dependent Responses in Rat Sensory Neurons
J Neurophysiol,
September 1, 2002;
88(3):
1387 - 1392.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. H. van Houwelingen, M. Kool, S. C. A. de Jager, F. A. M. Redegeld, D. van Heuven-Nolsen, A. D. Kraneveld, and F. P. Nijkamp
Mast Cell-Derived TNF-{alpha} Primes Sensory Nerve Endings in a Pulmonary Hypersensitivity Reaction
J. Immunol.,
May 15, 2002;
168(10):
5297 - 5302.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Szallasi and P. M. Blumberg
Vanilloid (Capsaicin) Receptors and Mechanisms
Pharmacol. Rev.,
June 1, 1999;
51(2):
159 - 212.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
