 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, January 1, 1998, 18(1):227-236
Tissue and Zonal-Specific Expression of an Olfactory Receptor Transgene
Pankaj Qasba and Randall R. Reed The Howard Hughes Medical Institute, Departments of Molecular Biology and Genetics and Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
Discrimination of odorants is thought to arise from the selective expression of one of a small number of individual receptors in any single olfactory neuron. Receptor genes are expressed in a small subset of neurons throughout a zonally restricted region of the sensory epithelium. We demonstrate that a 6.7 kb region upstream of the M4 olfactory receptor coding region was sufficient to direct expression in olfactory epithelium. Moreover, reporter expression recapitulated the zonal restriction and distributed neuronal expression observed for endogenous olfactory receptors. Transgenic lines were obtained that directed expression in two different receptor zones, one of which was identical to the endogenous M4 receptor. When the reporter was expressed in the same zone as the endogenous M4 receptor, the two expression patterns were, in large part, nonoverlapping. These results suggest a model in which important regulatory elements are located in close proximity to transcription initiation sites of the olfactory receptor genes and receive information defining zonal patterning via long-range processes.
Key words: transgene; receptors; zones; M4; sensory neurons; olfactory epithelium
Copyright © 1998 Society for Neuroscience 0270-6474/98/181227-10$05.00/0
This article has been cited by other articles:
J. C. McIntyre, S. C. Bose, A. J. Stromberg, and T. S. McClintock
Emx2 Stimulates Odorant Receptor Gene Expression
Chem Senses, November 1, 2008; 33(9): 825 - 837.
[Abstract] [Full Text] [PDF]
J. S. Michaloski, P. A.F. Galante, and B. Malnic
Identification of potential regulatory motifs in odorant receptor genes by analysis of promoter sequences
Genome Res., September 1, 2006; 16(9): 1091 - 1098.
[Abstract] [Full Text] [PDF]
B. M. Shykind
Regulation of odorant receptors: one allele at a time
Hum. Mol. Genet., April 15, 2005; 14(suppl_1): R33 - R39.
[Abstract] [Full Text] [PDF]
X. Zhang, M. Rogers, H. Tian, X. Zhang, D.-J. Zou, J. Liu, M. Ma, G. M. Shepherd, and S. J. Firestein
High-throughput microarray detection of olfactory receptor gene expression in the mouse
PNAS, September 28, 2004; 101(39): 14168 - 14173.
[Abstract] [Full Text] [PDF]
R. P. Lane, J. Young, T. Newman, and B. J. Trask
Species Specificity in Rodent Pheromone Receptor Repertoires
Genome Res., April 1, 2004; 14(4): 603 - 608.
[Abstract] [Full Text] [PDF]
S. S. Wang, J. W. Lewcock, P. Feinstein, P. Mombaerts, and R. R. Reed
Genetic disruptions of O/E2 and O/E3 genes reveal involvement in olfactory receptor neuron projection
Development, March 15, 2004; 131(6): 1377 - 1388.
[Abstract] [Full Text] [PDF]
J. W. Lewcock and R. R. Reed
A feedback mechanism regulates monoallelic odorant receptor expression
PNAS, January 27, 2004; 101(4): 1069 - 1074.
[Abstract] [Full Text] [PDF]
S. Serizawa, K. Miyamichi, H. Nakatani, M. Suzuki, M. Saito, Y. Yoshihara, and H. Sakano
Negative Feedback Regulation Ensures the One Receptor-One Olfactory Neuron Rule in Mouse
Science, December 19, 2003; 302(5653): 2088 - 2094.
[Abstract] [Full Text] [PDF]
R. Hoppe, H. Frank, H. Breer, and J. Strotmann
The Clustered Olfactory Receptor Gene Family 262: Genomic Organization, Promotor Elements, and Interacting Transcription Factors
Genome Res., December 1, 2003; 13(12): 2674 - 2685.
[Abstract] [Full Text] [PDF]
J. M. Young and B. J. Trask
The sense of smell: genomics of vertebrate odorant receptors
Hum. Mol. Genet., May 15, 2002; 11(10): 1153 - 1160.
[Abstract] [Full Text] [PDF]
B. Key and J. St John
Axon Navigation in the Mammalian Primary Olfactory Pathway: Where to Next?
Chem Senses, March 1, 2002; 27(3): 245 - 260.
[Abstract] [Full Text] [PDF]
R. P. Lane, J. C. Roach, I. Y. Lee, C. Boysen, A. Smit, B. J. Trask, and L. Hood
Genomic Analysis of the Olfactory Receptor Region of the Mouse and Human T-Cell Receptor alpha /delta Loci
Genome Res., January 1, 2002; 12(1): 81 - 87.
[Abstract] [Full Text] [PDF]
M. Pyrski, Z. Xu, E. Walters, D. J. Gilbert, N. A. Jenkins, N. G. Copeland, and F. L. Margolis
The OMP-lacZ Transgene Mimics the Unusual Expression Pattern of OR-Z6, a New Odorant Receptor Gene on Mouse Chromosome 6: Implication for Locus-Dependent Gene Expression
J. Neurosci., July 1, 2001; 21(13): 4637 - 4648.
[Abstract] [Full Text] [PDF]
R. P. Lane, T. Cutforth, J. Young, M. Athanasiou, C. Friedman, L. Rowen, G. Evans, R. Axel, L. Hood, and B. J. Trask
Genomic analysis of orthologous mouse and human olfactory receptor loci
PNAS, June 19, 2001; 98(13): 7390 - 7395.
[Abstract] [Full Text] [PDF]
M. Ma and G. M. Shepherd
Functional mosaic organization of mouse olfactory receptor neurons
PNAS, October 23, 2000; (2000) 220301797.
[Abstract] [Full Text]
S. J. Royal and B. Key
Development of P2 Olfactory Glomeruli in P2-Internal Ribosome Entry Site-Tau-LacZ Transgenic Mice
J. Neurosci., November 15, 1999; 19(22): 9856 - 9864.
[Abstract] [Full Text] [PDF]
P. Mombaerts
Seven-Transmembrane Proteins as Odorant and Chemosensory Receptors
Science, October 22, 1999; 286(5440): 707 - 711.
[Abstract] [Full Text]
A. Tsuboi, S.-i. Yoshihara, N. Yamazaki, H. Kasai, H. Asai-Tsuboi, M. Komatsu, S. Serizawa, T. Ishii, Y. Matsuda, F. Nagawa, et al.
Olfactory Neurons Expressing Closely Linked and Homologous Odorant Receptor Genes Tend to Project Their Axons to Neighboring Glomeruli on the Olfactory Bulb
J. Neurosci., October 1, 1999; 19(19): 8409 - 8418.
[Abstract] [Full Text] [PDF]
R. W. Friedrich and S. I. Korsching
Chemotopic, Combinatorial, and Noncombinatorial Odorant Representations in the Olfactory Bulb Revealed Using a Voltage-Sensitive Axon Tracer
J. Neurosci., December 1, 1998; 18(23): 9977 - 9988.
[Abstract] [Full Text] [PDF]
M. Ma and G. M. Shepherd
Functional mosaic organization of mouse olfactory receptor neurons
PNAS, November 7, 2000; 97(23): 12869 - 12874.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|