QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (44) Citing Articles via Google Scholar Google Scholar Articles by Agius, E. Articles by Cochard, P. Search for Related Content PubMed PubMed Citation Articles by Agius, E. Articles by Cochard, P.

 Previous Article  |  Next Article 

The Journal of Neuroscience, January 1, 1998, 18(1):328-338

Comparison of Neurite Outgrowth Induced by Intact and Injured Sciatic Nerves: A Confocal and Functional Analysis

Eric Agius and Philippe Cochard

Centre de Biologie du Développement, Centre National de la Recherche Scientifique Unité Mixte de Recherche 5547, affiliée à l'Institut National de la Santé et de la Recherche Médicale, Université Paul Sabatier, 31062 Toulouse, France

Mechanisms regulating axon growth in the peripheral nervous system have been studied by means of an in vitro bioassay, the tissue section culture, in which regenerating neurons are grown on substrata made up of tissue sections. Sections from intact and degenerated sciatic nerves proved to be different in their ability to support neurite outgrowth of embryonic chick sensory neurons from both qualitative and quantitative points of view. On denervated nerve sections, the total length of neurites elaborated per neuron was almost twice that found on intact nerve sections. In addition, confocal microscopy revealed a striking difference between intact and denervated nerve substrata: on denervated nerve sections, neurites grew inside the internal structures of endoneurial Schwann cell tubes, within the underlying tissue sections, whereas on intact nerve sections neurites extended along endoneurial basal laminae but never entered Schwann cell tubes. Perturbation experiments were used to analyze some of the molecular determinants that control neurite outgrowth in this system. Antibodies directed against the 1-integrin subunit inhibited neurite extension on both normal and degenerated rat sciatic nerve tissue. Strikingly, however, differential inhibition was observed using antibodies directed against extracellular matrix molecules. Anti-laminin-2 (merosin) antibodies drastically reduced both the percentage of growing neurons and the total length of neurites on denervated nerve sections, but they did not modify these parameters on sections of normal nerve. Taken together, these results suggest that laminin-2/merosin promotes neurite outgrowth in peripheral nerve environments but only after Wallerian degeneration, which is when axons are allowed to extend within endoneurial tubes.

Key words: nerve fiber growth; axon regeneration; peripheral nervous system; Wallerian degeneration; rat sciatic nerve; extracellular matrix; integrin; merosin; cryoculture; bioassay; confocal microscopy

---

Copyright © 1998 Society for Neuroscience  0270-6474/98/181328-11$05.00/0

This article has been cited by other articles:

				B. Duran-Jimenez, D. Dobler, S. Moffatt, N. Rabbani, C. H. Streuli, P. J. Thornalley, D. R. Tomlinson, and N. J. Gardiner Advanced Glycation End Products in Extracellular Matrix Proteins Contribute to the Failure of Sensory Nerve Regeneration in Diabetes Diabetes, December 1, 2009; 58(12): 2893 - 2903. [Abstract] [Full Text] [PDF]

				C. A. Krekoski, D. Neubauer, J. B. Graham, and D. Muir Metalloproteinase-Dependent Predegeneration In Vitro Enhances Axonal Regeneration within Acellular Peripheral Nerve Grafts J. Neurosci., December 1, 2002; 22(23): 10408 - 10415. [Abstract] [Full Text] [PDF]

				R H M King The role of glycation in the pathogenesis of diabetic polyneuropathy Mol. Pathol., December 1, 2001; 54(6): 400 - 408. [Abstract] [Full Text] [PDF]

				M. G. Vogelezang, Z. Liu, J. B. Relvas, G. Raivich, S. S. Scherer, and C. ffrench-Constant {alpha}4 Integrin Is Expressed during Peripheral Nerve Regeneration and Enhances Neurite Outgrowth J. Neurosci., September 1, 2001; 21(17): 6732 - 6744. [Abstract] [Full Text] [PDF]

				H. Yang, X. Wang, and M. K. Raizada Characterization of Signal Transduction Pathway in Neurotropic Action of Angiotensin II in Brain Neurons Endocrinology, August 1, 2001; 142(8): 3502 - 3511. [Abstract] [Full Text] [PDF]

				A. Werner, M. Willem, L. L. Jones, G. W. Kreutzberg, U. Mayer, and G. Raivich Impaired Axonal Regeneration in alpha 7 Integrin-Deficient Mice J. Neurosci., March 1, 2000; 20(5): 1822 - 1830. [Abstract] [Full Text] [PDF]

				T. M. Brushart, J. Gerber, P. Kessens, Y.-G. Chen, and R. M. Royall Contributions of Pathway and Neuron to Preferential Motor Reinnervation J. Neurosci., November 1, 1998; 18(21): 8674 - 8681. [Abstract] [Full Text] [PDF]

				J. Zuo, T. A. Ferguson, Y. J. Hernandez, W. G. Stetler-Stevenson, and D. Muir Neuronal Matrix Metalloproteinase-2 Degrades and Inactivates a Neurite-Inhibiting Chondroitin Sulfate Proteoglycan J. Neurosci., July 15, 1998; 18(14): 5203 - 5211. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help