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The Journal of Neuroscience, January 1, 1998, 18(1):458-466
Involvement of Presynaptic and Postsynaptic Mechanisms in a Cellular Analog of Classical Conditioning at Aplysia Sensory-Motor Neuron Synapses in Isolated Cell Culture
Jian-Xin Bao1, Eric R. Kandel1, 2, 3, and Robert D. Hawkins1, 2 1 Center for Neurobiology and Behavior,
College of Physicians and Surgeons, Columbia University, 2 New York State Psychiatric Institute, and 3 Howard Hughes Medical Institute, New York, New York 10032 Temporal pairing of presynaptic activity and serotonin produces enhanced facilitation at Aplysia sensory-motor neuron synapses (pairing-specific facilitation), which may contribute to classical conditioning of the gill and siphon withdrawal reflex. This cellular analog of conditioning is thought to involve Ca2+ priming of the cAMP pathway in the sensory neurons. Consistent with that idea, we have found that pairing-specific facilitation by serotonin is greatly reduced by presynaptic injection of a slow Ca2+ chelator or a specific inhibitor of cAMP-dependent protein kinase and is accompanied by a transient increase in the frequency but by no change in the amplitude of spontaneous, miniature EPSPs. However, like post-tetanic potentiation (PTP) and long-term potentiation (LTP) at these synapses, pairing-specific facilitation is also greatly reduced by postsynaptic injection of a rapid Ca2+ chelator or by postsynaptic hyperpolarization during training, although postsynaptic hyperpolarization has no effect on the increase in frequency or on the amplitude of spontaneous EPSPs. These results suggest that pairing-specific facilitation by serotonin involves Hebbian postsynaptic as well as non-Hebbian presynaptic components that interact in some way, perhaps via retrograde signaling, to specifically enhance evoked, synchronized release of transmitter.
Key words: Aplysia; pairing-specific facilitation; serotonin; sensory neuron; motor neuron; cell culture; calcium; cAMP-dependent protein kinase
Copyright © 1998 Society for Neuroscience 0270-6474/98/181458-09$05.00/0
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