 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, May 15, 1998, 18(10):3708-3714
Nitric Oxide-Dependent Production of cGMP Supports the Survival of Rat Embryonic Motor Neurons Cultured with Brain-Derived Neurotrophic Factor
Alvaro G. Estévez1, 6, 8, Nathan Spear1, 6, J. Anthony Thompson2, 4, 6, Trudy L. Cornwell5, Rafael Radi7, Luis Barbeito8, 9, and Joseph S. Beckman1, 2, 3, 6 Departments of 1 Anesthesiology, 2 Biochemistry and Molecular Genetics, 3 Neuroscience, 4 Surgery, and 5 Pathology,
Division of Molecular and Cellular Pathology, and 6 The University of Alabama at Birmingham Center for Free Radical Biology, The University of Alabama at Birmingham, Birmingham, Alabama 35233, and 7 Departamento de Bioquímica, Facultad de Medicina, 8 Sección Neurociencias, Facultad de Ciencias, Universidad de la República 11200 Montevideo, Uruguay, and 9 División Neurobiología Celular y Molecular, Instituto Clemente Estable, 11600 Montevideo, Uruguay Trophic factor deprivation induces neuronal nitric oxide synthase (NOS) and apoptosis of rat embryonic motor neurons in culture. We report here that motor neurons constitutively express endothelial NOS that helps support the survival of motor neurons cultured with brain-derived neurotrophic factor (BDNF) by activating the nitric oxide-dependent soluble guanylate cyclase. Exposure of BDNF-treated motor neurons to nitro-L-arginine methyl ester (L-NAME) decreased cell survival 40-50% 24 hr after plating. Both low steady-state concentrations of exogenous nitric oxide (<0.1 µM) and cGMP analogs protected BDNF-treated motor neurons from death induced by L-NAME. Equivalent concentrations of cAMP analogs did not affect cell survival. Inhibition of nitric oxide-sensitive guanylate cyclase with 2 µM 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) reduced the survival of BDNF-treated motor neurons by 35%. cGMP analogs also protected from ODQ-induced motor neuron death, whereas exogenous nitric oxide did not. In all cases, cell death was prevented with caspase inhibitors. Our results suggest that nitric oxide-stimulated cGMP synthesis helps to prevent apoptosis in BDNF-treated motor neurons.
Key words: motor neurons; BDNF; endothelial nitric oxide synthase; nitric oxide; apoptosis; guanylate cyclase soluble; cGMP
Copyright © 1998 Society for Neuroscience 0270-6474/98/18103708-07$05.00/0
This article has been cited by other articles:
A. G. Estevez, M. A. Sahawneh, P. S. Lange, N. Bae, M. Egea, and R. R. Ratan
Arginase 1 Regulation of Nitric Oxide Production Is Key to Survival of Trophic Factor-Deprived Motor Neurons.
J. Neurosci., August 15, 2006; 26(33): 8512 - 8516.
[Abstract] [Full Text] [PDF]
P. Chanvorachote, U. Nimmannit, L. Wang, C. Stehlik, B. Lu, N. Azad, and Y. Rojanasakul
Nitric Oxide Negatively Regulates Fas CD95-induced Apoptosis through Inhibition of Ubiquitin-Proteasome-mediated Degradation of FLICE Inhibitory Protein
J. Biol. Chem., December 23, 2005; 280(51): 42044 - 42050.
[Abstract] [Full Text] [PDF]
K.-S. HA, K.-M. KIM, Y.-G. KWON, S.-K. BAI, W.-D. NAM, Y.-M. YOO, P. K. M. KIM, H.-T. CHUNG, T. R. BILLIAR, and Y.-M. KIM
Nitric oxide prevents 6-hydroxydopamine-induced apoptosis in PC12 cells through cGMP-dependent PI3 kinase/Akt activation
FASEB J, June 1, 2003; 17(9): 1036 - 1047.
[Abstract] [Full Text] [PDF]
E. Ciani, S. Guidi, G. Della Valle, G. Perini, R. Bartesaghi, and A. Contestabile
Nitric Oxide Protects Neuroblastoma Cells from Apoptosis Induced by Serum Deprivation through cAMP-response Element-binding Protein (CREB) Activation
J. Biol. Chem., December 13, 2002; 277(51): 49896 - 49902.
[Abstract] [Full Text] [PDF]
X.-m. Liu, G. B Chapman, K. J Peyton, A. I Schafer, and W. Durante
Carbon monoxide inhibits apoptosis in vascular smooth muscle cells
Cardiovasc Res, August 1, 2002; 55(2): 396 - 405.
[Abstract] [Full Text] [PDF]
V. Abudara, A. F. Alvarez, M. H. Chase, and F. R. Morales
Nitric Oxide as an Anterograde Neurotransmitter in the Trigeminal Motor Pool
J Neurophysiol, July 1, 2002; 88(1): 497 - 506.
[Abstract] [Full Text] [PDF]
K. Takuma, P. Phuagphong, E. Lee, K. Mori, A. Baba, and T. Matsuda
Anti-apoptotic Effect of cGMP in Cultured Astrocytes. INHIBITION BY cGMP-DEPENDENT PROTEIN KINASE OF MITOCHONDRIAL PERMEABLE TRANSITION PORE
J. Biol. Chem., December 14, 2001; 276(51): 48093 - 48099.
[Abstract] [Full Text] [PDF]
G.-W. Lu and H.-Y. Liu
Downregulation of nitric oxide in the brain of mice during their hypoxic preconditioning
J Appl Physiol, September 1, 2001; 91(3): 1193 - 1198.
[Abstract] [Full Text] [PDF]
T. Polte, A. Abate, P. A. Dennery, and H. Schroder
Heme Oxygenase-1 Is a cGMP-Inducible Endothelial Protein and Mediates the Cytoprotective Action of Nitric Oxide
Arterioscler Thromb Vasc Biol, May 1, 2000; 20(5): 1209 - 1215.
[Abstract] [Full Text] [PDF]
J. Li, S. Yang, and T. R. Billiar
Cyclic Nucleotides Suppress Tumor Necrosis Factor alpha -Mediated Apoptosis by Inhibiting Caspase Activation and Cytochrome c Release in Primary Hepatocytes via a Mechanism Independent of Akt Activation
J. Biol. Chem., April 21, 2000; 275(17): 13026 - 13034.
[Abstract] [Full Text] [PDF]
T. L. Williamson, L. B. Corson, L. Huang, A. Burlingame, J. Liu, L. I. Bruijn, D. W. Cleveland;, J. S. Beckman, J. P. Crow, and A. G. Estévez;
Toxicity of ALS-Linked SOD1 Mutants
Science, April 21, 2000; 288(5465): 399a - 399.
[Full Text]
A. G. Estévez, J. P. Crow, J. B. Sampson, C. Reiter, Y. Zhuang, G. J. Richardson, M. M. Tarpey, L. Barbeito, and J. S. Beckman
Induction of Nitric Oxide -- Dependent Apoptosis in Motor Neurons by Zinc-Deficient Superoxide Dismutase
Science, December 24, 1999; 286(5449): 2498 - 2500.
[Abstract] [Full Text]
J. Morgan, J. Caprioli, and Y. Koseki
Nitric Oxide Mediates Excitotoxic and Anoxic Damage in Rat Retinal Ganglion Cells Cocultured With Astroglia
Arch Ophthalmol, November 1, 1999; 117(11): 1524 - 1529.
[Abstract] [Full Text] [PDF]
J. S. Beckman
Parsing the Effects of Nitric Oxide, S-Nitrosothiols, and Peroxynitrite on Inducible Nitric Oxide Synthase-Dependent Cardiac Myocyte Apoptosis
Circ. Res., October 29, 1999; 85(9): 870 - 871.
[Full Text] [PDF]
Y.-M. Kim, H.-T. Chung, S.-S. Kim, J.-A Han, Y.-M. Yoo, K.-M. Kim, G.-H. Lee, H.-Y. Yun, A. Green, J. Li, et al.
Nitric Oxide Protects PC12 Cells from Serum Deprivation-Induced Apoptosis by cGMP-Dependent Inhibition of Caspase Signaling
J. Neurosci., August 15, 1999; 19(16): 6740 - 6747.
[Abstract] [Full Text] [PDF]
J. Li, C. A. Bombeck, S. Yang, Y.-M. Kim, and T. R. Billiar
Nitric Oxide Suppresses Apoptosis via Interrupting Caspase Activation and Mitochondrial Dysfunction in Cultured Hepatocytes
J. Biol. Chem., June 11, 1999; 274(24): 17325 - 17333.
[Abstract] [Full Text] [PDF]
J. B. Mannick, A. Hausladen, L. Liu, D. T. Hess, M. Zeng, Q. X. Miao, L. S. Kane, A. J. Gow, and J. S. Stamler
Fas-Induced Caspase Denitrosylation
Science, April 23, 1999; 284(5414): 651 - 654.
[Abstract] [Full Text]
L. Rossig, B. Fichtlscherer, K. Breitschopf, J. Haendeler, A. M. Zeiher, A. Mulsch, and S. Dimmeler
Nitric Oxide Inhibits Caspase-3 by S-Nitrosation in Vivo
J. Biol. Chem., March 12, 1999; 274(11): 6823 - 6826.
[Abstract] [Full Text] [PDF]
F. M. Inglis, F. Furia, K. E. Zuckerman, S. M. Strittmatter, and R. G. Kalb
The Role of Nitric Oxide and NMDA Receptors in the Development of Motor Neuron Dendrites
J. Neurosci., December 15, 1998; 18(24): 10493 - 10501.
[Abstract] [Full Text] [PDF]
Y.-G. Kwon, J.-K. Min, K.-M. Kim, D.-J. Lee, T. R. Billiar, and Y.-M. Kim
Sphingosine 1-Phosphate Protects Human Umbilical Vein Endothelial Cells from Serum-deprived Apoptosis by Nitric Oxide Production
J. Biol. Chem., March 30, 2001; 276(14): 10627 - 10633.
[Abstract] [Full Text] [PDF]
C. D. Reiter, R.-J. Teng, and J. S. Beckman
Superoxide Reacts with Nitric Oxide to Nitrate Tyrosine at Physiological pH via Peroxynitrite
J. Biol. Chem., October 13, 2000; 275(42): 32460 - 32466.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|