QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (28) Citing Articles via Google Scholar Google Scholar Articles by Battelle, B.-A. Articles by Smith, W. C. Search for Related Content PubMed PubMed Citation Articles by Battelle, B.-A. Articles by Smith, W. C. Pubmed/NCBI databases Protein Substance via MeSH

 Previous Article  |  Next Article 

The Journal of Neuroscience, June 15, 1998, 18(12):4548-4559

A Myosin III from Limulus Eyes Is a Clock-Regulated Phosphoprotein

Barbara-Anne Battelle¹, Anne W. Andrews¹, Bruce G. Calman¹, James R. Sellers², Robert M. Greenberg¹, and W. Clay Smith¹

¹ Whitney Laboratory and Department of Neuroscience, University of Florida, St. Augustine, Florida 32086, and ² Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-1762

The lateral eyes of the horseshoe crab Limulus polyphemus undergo dramatic daily changes in structure and function that lead to enhanced retinal sensitivity and responsiveness to light at night. These changes are controlled by a circadian neural input that alters photoreceptor and pigment cell shape, pigment migration, and phototransduction. Clock input to the eyes also regulates photomechanical movements within photoreceptors, including membrane shedding. The biochemical mechanisms underlying these diverse effects of the clock on the retina are unknown, but a major biochemical consequence of activating clock input to the eyes is a rise in the concentration of cAMP in photoreceptors and the phosphorylation of a 122 kDa visual system-specific protein. We have cloned and sequenced cDNA encoding the clock-regulated 122 kDa phosphoprotein and show here that it is a new member of the myosin III family. We report that Limulus myosin III is similar to other unconventional myosins in that it binds to calmodulin in the absence of Ca²⁺; it is novel in that it is phosphorylated within its myosin globular head, probably by cAMP-dependent protein kinase. The protein is present throughout the photoreceptor, including the region occupied by the photosensitive rhabdom. We propose that the phosphorylation of Limulus myosin III is involved in one or more of the structural and functional changes that occur in Limulus eyes in response to clock input.

Key words: myosin III; ninaC; Limulus polyphemus; photoreceptor cells; circadian rhythms; octopamine; unconventional myosin; cytoskeleton; Drosophila melanogaster; cAMP-dependent phosphorylation

---

Copyright © 1998 Society for Neuroscience  0270-6474/98/18124548-12$05.00/0

This article has been cited by other articles:

				A. C. Dose, S. Ananthanarayanan, J. E. Moore, B. Burnside, and C. M. Yengo Kinetic Mechanism of Human Myosin IIIA J. Biol. Chem., January 5, 2007; 282(1): 216 - 231. [Abstract] [Full Text] [PDF]

				T. Kambara, S. Komaba, and M. Ikebe Human Myosin III Is a Motor Having an Extremely High Affinity for Actin J. Biol. Chem., December 8, 2006; 281(49): 37291 - 37301. [Abstract] [Full Text] [PDF]

				S. Komaba, A. Inoue, S. Maruta, H. Hosoya, and M. Ikebe Determination of Human Myosin III as a Motor Protein Having a Protein Kinase Activity J. Biol. Chem., June 6, 2003; 278(24): 21352 - 21360. [Abstract] [Full Text] [PDF]

				A. C. Dose, D. W. Hillman, C. Wong, L. Sohlberg, J. Lin-Jones, and B. Burnside Myo3A, One of Two Class III Myosin Genes Expressed in Vertebrate Retina, Is Localized to the Calycal Processes of Rod and Cone Photoreceptors and Is Expressed in the Sacculus Mol. Biol. Cell, March 1, 2003; 14(3): 1058 - 1073. [Abstract] [Full Text] [PDF]

				K. G. Patel, C. Liu, P. L. Cameron, and R. S. Cameron Myr 8, A Novel Unconventional Myosin Expressed during Brain Development Associates with the Protein Phosphatase Catalytic Subunits 1{alpha} and 1{gamma}1 J. Neurosci., October 15, 2001; 21(20): 7954 - 7968. [Abstract] [Full Text] [PDF]

				S. Chyb, W. Hevers, M. Forte, W. J. Wolfgang, Z. Selinger, and R. C. Hardie Modulation of the Light Response by cAMP in Drosophila Photoreceptors J. Neurosci., October 15, 1999; 19(20): 8799 - 8807. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help