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The Journal of Neuroscience, July 15, 1998, 18(14):5294-5300
Comparison of Hippocampal Dendritic Spines in Culture and in Brain
Christopher Boyer, Thomas Schikorski, and Charles F. Stevens Molecular Neurobiology Laboratory, and Howard Hughes Medical Institute at The Salk Institute, La Jolla, California 92037
We have quantified hippocampal spine structure at the light and ultrastructural levels in cell cultures ~1- 3 weeks old and in the brains of rodents 5 and 21 d old. The number of spines bearing synapses increases with age in cultures and in brain, but the structures are similar in both. In culture, about half of the synapses are formed on spines and the remainder are formed on dendritic shafts. In the 5-d-old brain, about half of the synapses occur on dendritic shafts, by 3 weeks of age only ~20% of synapses are found on dendritic shafts, and in the adult shaft synapses are very rare.
Key words: synapse; culture; ultrastructure; spines; hippocampus; morphometry
Copyright © 1998 Society for Neuroscience 0270-6474/98/18145294-07$05.00/0
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