The Journal of Neuroscience, August 1, 1998, 18(15):6057-6057
ERRATUM
Correction
In the article "Dopamine Neurons Make Glutamatergic Synapses
In Vitro," by D. Sulzer, M. P. Joyce. L. Lin, D. Geldwert,
S. N. Haber, T. Hattori, and S. Rayport, which appeared on
pages 4588-4602 of the June 15, 1998 issue, the resolution
Figure 6 (page 4597) was unsatisfactory. The figure and legend are
reprinted.
View larger version (131K):
[in this window]
[in a new window]
|
Figure 6.
Ultrastructure of a DA neuron in single cell
microculture. Sections are shown from a single DA neuron grown in
microcultures and TH-stained using DAB (inset). To
maximize antibody penetration, we used a relatively high detergent
concentration. Although this solubilized membranes, resulting in an
apparent degradation of the quality of ultrastructural preservation, it
enhanced the visualization of synaptic specializations.
A, In the cell body, TH staining was distributed in a
patchy pattern throughout weakly stained cytoplasm. TH+
processes emerged from intensely stained regions (filled
arrows); nearby, TH processes emerged from
unstained regions (open arrows). B,
Within the neuropil, distinctly stained and unstained processes
intermingled with each other; in some cases within a single process, a
stained portion (filled arrow) was clearly
distinguishable from an unstained portion (open arrow).
C, Single TH-immunoreactive neurons formed morphological
synapses on themselves (autapses). Those autapses were in close
proximity to the cell body (as seen at the light level; Fig. 5). In
this cell, a total of eight autapses with clear postsynaptic
specializations were identified after serial sectioning; one autapse
showed presynaptic TH staining and had symmetric synaptic membrane
specializations. D, The other autapses had asymmetric
synaptic specializations with no detectable immunostaining of the
presynaptic elements. Two of the seven TH boutons (data
not shown) made asymmetric synaptic contacts with TH+
dendritic elements. TH+ varicosities at a distance from the
cell body had accumulations of synaptic vesicles but lacked presynaptic
or postsynaptic densities (data not shown).
|
|
Copyright © 1998 Society for Neuroscience 0270-6474/98/18156057-01$05.00/0
